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EMAGE:6642

Fam48a family with sequence similarity 48, member A ( MGI:1929651)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642
"Pseudo-wholemount" of euxassay_002226. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002226_01 euxassay_002226_02 euxassay_002226_03 euxassay_002226_04
EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642
euxassay_002226_05 euxassay_002226_06 euxassay_002226_07 euxassay_002226_08 euxassay_002226_09
EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642
euxassay_002226_10 euxassay_002226_11 euxassay_002226_12 euxassay_002226_13 euxassay_002226_14
EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642 EMAGE:6642
euxassay_002226_15 euxassay_002226_16 euxassay_002226_17 euxassay_002226_18 euxassay_002226_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6642Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6642_wholemount_strong.wlz
6642_wholemount_moderate.wlz
6642_wholemount_weak.wlz
6642_wholemount_possible.wlz
6642_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6642_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
stomach
strong strong
regionalstrong expression: see section 03 moderate expression: see section 04 05 06 07 08 09
hindgut
moderate moderate
regionalmoderate expression: see section 12
rectum
moderate moderate
regionalmoderate expression: see section 12
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1950
Entity Detected:Fam48a, family with sequence similarity 48, member A ( MGI:1929651)
Sequence:sense strand is shown

>T1950
NANAAAATGACNGCCAAATGTCTCCATCCCACTCCTCTGCTGATGACCATTCAAACTGGTTTGTCATTGG
GTCAAAGACTGACGCAGAGAGGGTAGTTAATCAATACCAGGAGTTGGTCCAGAATGAAGCCAAATGCCCA
GTGAAGATGTCACACAGCAGTAGTGGTTCAGCCGCTCTCAGTCCAGGGGAGGAGGCAGAACAGGCGGAGA
CCTCGTCCATTCAGTCTTCAGTTTTGGGAAAGGGAGTAAAACACCGACCCCCACCCATCAAACTTCCCTC
AGGCTCAGGAAATAGTTCCTCAGGTAACTATTTCACAGCACAGCAGGCCAGCAGCTTCCTTAAATCTCCA
ACTCCTCCTCCATCGTGTAAGCCAAGCCTTTCTCGGAAGTCTTCTGTGGACCTCAGTCAAGTTAGCATGC
TTTCTCCTGCCGCCCTGTCCCCTGCCAGCTCATCCCAGAGACATGAAAGCTAAAAAGGAAACACCTCAAG
AGCTTTGCTTTACTTTTT
Notes:The probe template was PCR amplified from IMAGE:639666 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:639666 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BL6/SV129
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6645 same embryo
 EMAGE:6641 same embryo
 EMAGE:6643 same embryo
 EMAGE:6644 same embryo
 EurExpress:euxassay_002226 same experiment
 MGI:4824745 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS