EMAGE mouse gene expression spatial database

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EMAGE:6960

Pisd phosphatidylserine decarboxylase ( MGI:2445114)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002547. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002547_01	euxassay_002547_02	euxassay_002547_03	euxassay_002547_04

euxassay_002547_05	euxassay_002547_06	euxassay_002547_07	euxassay_002547_08	euxassay_002547_09

euxassay_002547_10	euxassay_002547_11	euxassay_002547_12	euxassay_002547_13	euxassay_002547_14

euxassay_002547_15	euxassay_002547_16	euxassay_002547_17	euxassay_002547_18	euxassay_002547_19

euxassay_002547_20	euxassay_002547_21	euxassay_002547_22	euxassay_002547_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6960_wholemount_strong.wlz

6960_wholemount_moderate.wlz

6960_wholemount_weak.wlz

6960_wholemount_possible.wlz

6960_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6960_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 17 18 19 20 21 22 23

spinal cord

strong

homogeneous

strong expression: see section 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 10 11 15 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4053
Entity Detected:	Pisd, phosphatidylserine decarboxylase ( MGI:2445114)
Sequence:	sense strand is shown >T4053 GGAGGAGGCGAGATGGCGGCGTCTGGGGGCCGCGCGTGTGTGCGCTCGCTTCGTGGAGGTGTGCTGTGGC GGAGCAGCCCCTGTCACTATGAATCTACTGCCACACGCCATTTTCTGGGGACCTTACAGAAATTGCCTCT TCAGGCAGGTGTCAGAAATTTCCATACTGCTCCTGTCCGATCCTTGTTCCTGCTGCGCCCTGTGCCCATC CTGTTGGCAACAGGTGGAGGGTATGCCGGGTACCGGCAGTATGAGAAGTACAGGGAACGGAAGCTTGAGA AGCTGGGCTTGGAGATCCCGCCCAAACTTGCTAGTCACTGGGAGGTGTCCCTGTATAAATCTGTGCCAAC GCGTTTGCTGTCACGTGCCTGTGGTCGCCTCAACCAAGTAGAACTTCCTTACTGGCTCCGGAGGCCAGTC TACAGCCTGTATATCTGGACCTTTGGGGTGAACATGACTGAGGCTGCAGTGGAGGACCTGCACCACTACC GCAACCTCAGCGAGTTCTTCCGGCGTAAGCTGAAGCCTCAGGCACGGCCCGTATGTGGCTTGCACTGTGT GACCAGCCCATCAGATGGCAAGATCCTCACCTTTGGGCAGGTGAAGAACTCTGAGGTGGAGCAGGTAAAG GGCGTAACCTACTCCTTGGAGTCCTTCCTGGGCCCTCGAGCCAATACAGAGGACCTGCCCTTCCCTCCAG CTTCCTCCAGTGACTCCTTCAGAAACCAGCTGGTCACCCGGGAAGGGAATGAGCTGTACCACTGTGTCAT CTACCTGGCCCCAGGAGACTACCACTGCTTCCACTCCCCCACTGACTGGACCATCTCACATCGGCGCCAC TTCCCAGGTTCCCTGATGTCAGTGAACCCTGGCATGGCCCGATGGATCAAAGAGCTCTTTTGTCACAATG AGCGTGTAGTCCTAACTGGAGACTGGAAACATGGGTTCTTCTCGTTGACAGCAGTGGGAGCCACCAATGT GGGCTCTATCCGTATCCACTTTGACCGAGACCTGCACACAAACAGCCCCAGGTACAGCAAGGGCTCCTAC AATGACCTGAGCTTTGTAACACATGCCAACAAGGAGGGCATTCCCATGCGCAAGGGTGAGCCCCTGGGGG AGTTCAACCTGGGCTCCACCATCGTGCTCATCTTCGAGGCACCCAAGGACTTCAACTTCAGGCTGAAGGC AGGGCAGAAGATCCGCTTTGGGGAAGCACTGGGCTCCCTCTAGTCCTCTCGGTTGTGGCTGCCAAAGGAT CTTTCCACACAGGAGCATGAGGGTCTTCAGCGGAGCCTCAGGCTGTCTGGGTGAGGGGCACTGCGGGGCT GACCTTATAAGATTGCCTGTCCATGGGTGCAGAGGAGAGGTTCTGGGTCTCTCAGGACAGGACCTAGACC CCTTTTCAGAGTATGGGTGGGTGTGATTTGACCCCAAAGTCACTTTCAGGGGTGTTTTAAATTTTTGTAC AAGCCAGAGCCTCCCACCCCTAACTCCTGGCTGTGTACTACCACACCCTCTAGCTGTGACCCCTCCCGTC ACAGGGCTCTCTCAATGTGCACAGGGTGAGGTGCCTGCTGGGCACAGACTGTGGATCCACTGTCTTCCTA GAGCAGGCCACATGGCCTTCATAACTGCTTTCTGCTCCCCAATTTCACCCGTCTGCTTTCTGGAAAAGTA AAATCATTTATATAATGCTGAATGTGTTATCCTGGGCTCTGCCAGTGTCTTCTGAGGACAATGACTAATG ATGTAGACCTTTGTAGGCTCTAAGAAGCTGGCTGAATCCTTTTGCTGGTAGGCAAAACCAATTAATTACT GGCTTGTCTGGTAGAGTCTGGAGTGAAAGGGCAGGTGGAGGCCCTGCCCTGCAGAACTAGGGGACATGCT GCTATTGGTCTAGGGACTGTGTTGGAGGCAGAGGGTATAGAGGTCAGTACCTAGAAAACAGGTATGCAGG CCATGGGGAACCCAGTGGTAGGGACAAGACCAGAGTTCATGTCGATCTTGGGAATGCCATGAATTTCTCC TGCAGGAGAAATCACTTAACTTTTTCAACTGTATCAGTAGTACCGTATTTTGTATGAAAAGTAGGAGACT TCTGAACAGTAATTCATTTCATTGCAAGACATTTTGAAATTAAAAAAACAAAACAAAAACTAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6809528 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6809528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6959 same embryo
	EMAGE:6961 same embryo
	EMAGE:6962 same embryo
	EurExpress:euxassay_002547 same experiment
	MGI:4827219 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source