EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6997

Ncapg2 non-SMC condensin II complex, subunit G2 ( MGI:1923294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002384. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002384_01	euxassay_002384_02	euxassay_002384_03	euxassay_002384_04

euxassay_002384_05	euxassay_002384_06	euxassay_002384_07	euxassay_002384_08	euxassay_002384_09

euxassay_002384_10	euxassay_002384_11	euxassay_002384_12	euxassay_002384_13	euxassay_002384_14

euxassay_002384_15	euxassay_002384_16	euxassay_002384_17	euxassay_002384_18	euxassay_002384_19

euxassay_002384_20	euxassay_002384_21	euxassay_002384_22	euxassay_002384_23	euxassay_002384_24

euxassay_002384_25	euxassay_002384_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6997_wholemount_strong.wlz

6997_wholemount_moderate.wlz

6997_wholemount_weak.wlz

6997_wholemount_possible.wlz

6997_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6997_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 03 04 05 06 07 20 21 22 23

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 10 11 15 16 17

telencephalon ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 19 20 24 weak expression: see section 02 03 04 05 06 21 22 23

nasal cavity epithelium

moderate

regional

moderate expression: see section 07

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 14 15 17 18 moderate expression: see section 07 08 09 10 16

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1995
Entity Detected:	Ncapg2, non-SMC condensin II complex, subunit G2 ( MGI:1923294)
Sequence:	sense strand is shown >T1995 GGCCTCGAGCCAGATTCGGCACGAGGGANAAGTCACAGTGCTGTTTGACTTAGTCCAGGAAGTGTTTCAG AAGATGTTGGAATGTATTGCCTGTATCTTCAGGAAGCAACCTGAAGAAAGTCTTCCGCTCTTTCATTCTG TTCAGACCCCTCTTCATGAATTCATCACCACCATCCAGTCCTGGCACAAGGACACTGCAGTGCACCATGC TGTGCTTTCTACGTTGATTGCTGCACCTGTGGTTGAGATAAGTCACCAGCTGCAGAAGGTTTCTGATATA GAAGAGCTGACCTCACCACAATGCCTTCATGACCTTCCTCCATTTTCAAGGTGTTTAGTGGGAGTGATCA TGAAGTCTTCGGATGTGGTCAGGTCATTTTTGGATGAACTGAAGGCATGTGTAACTTCTGGTGATGTTGA AGGCATTGTGTGCCTCACAGCTGTTCTGCATATTATTTTGGTTATTAACAAAGGTAAACATATAAGTGCA AAGGTGAAGGAAGTTGCAACAGCTGTGTACAGAAAGCTGAAGACCTTTATGGAGATCACTTTG
Notes:	The probe template was PCR amplified from IMAGE:721107 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:721107 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6996 same embryo
	EMAGE:6995 same embryo
	EMAGE:6999 same embryo
	EMAGE:6994 same embryo
	EMAGE:6998 same embryo
	EurExpress:euxassay_002384 same experiment
	MGI:4826612 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source