EMAGE mouse gene expression spatial database

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EMAGE:7165

Herc2 hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 2 ( MGI:103234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002598. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002598_01	euxassay_002598_02	euxassay_002598_03	euxassay_002598_04

euxassay_002598_05	euxassay_002598_06	euxassay_002598_07	euxassay_002598_08	euxassay_002598_09

euxassay_002598_10	euxassay_002598_11	euxassay_002598_12	euxassay_002598_13	euxassay_002598_14

euxassay_002598_15	euxassay_002598_16	euxassay_002598_17	euxassay_002598_18	euxassay_002598_19

euxassay_002598_20	euxassay_002598_21	euxassay_002598_22	euxassay_002598_23	euxassay_002598_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7165_wholemount_strong.wlz

7165_wholemount_moderate.wlz

7165_wholemount_weak.wlz

7165_wholemount_possible.wlz

7165_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7165_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22 23 24

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 10 19 20

spinal cord

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 11

cervical ganglion

moderate

regional

moderate expression: see section 18 19 weak expression: see section 10

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 15 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2066
Entity Detected:	Herc2, hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 2 ( MGI:103234)
Sequence:	sense strand is shown >T2066 TGGCCTCGAGCAGATTCGGCACGAGGGTAAAGGTGGGTTAGCTGGCCCTGATGGCACCAAGTCTGTCTTT GGGCAGATGTGTGCTAAGATGAGTTCCTTCAGTCCTGACAGCCTTCTCCTTCCTCACCGAGTCTGGAAAG TCAAGTTTGTGGGTGAATCCGTGGACGACTGTGGTGGTGGCTACAGTGAGTCTATAGCAGAGATTTGTGA GGAGCTGCAAAACGGACTCACACCTCTTCTGATTGTGACTCCCAATGGCAGGGATGAGTCTGGTGCCAAC AGAGACTGTTACCTATTAAACCCTGCCACCCGTGCACCCGTGCACTGCAGCATGTTCCGATTCCTAGGGG TGTTATTGGGCATTGCCATCCGAACTGGGAGTCCACTAAGTTTGAATCTTGCTGAACCTGTATGGAAGCA GCTGGCTGGGATGAGCCTCACCATTGCAGACCTGAGTGAGGTAGATAAAGATTTTATTCCTGGGCTTATG TACATTCGTGACAATGAAGCCACTTCAGAGGAATTTGAGGCTATGAGCCTGCCC
Notes:	The probe template was PCR amplified from IMAGE:820814 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:820814 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7164 same embryo
	EMAGE:7163 same embryo
	EMAGE:7166 same embryo
	EurExpress:euxassay_002598 same experiment
	MGI:4825356 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source