EMAGE mouse gene expression spatial database

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EMAGE:7322

Tspyl4 TSPY-like 4 ( MGI:106393)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004360. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004360_01	euxassay_004360_02	euxassay_004360_03	euxassay_004360_04

euxassay_004360_05	euxassay_004360_06	euxassay_004360_07	euxassay_004360_08	euxassay_004360_09

euxassay_004360_10	euxassay_004360_11	euxassay_004360_12	euxassay_004360_13	euxassay_004360_14

euxassay_004360_15	euxassay_004360_16	euxassay_004360_17	euxassay_004360_18	euxassay_004360_19

euxassay_004360_20	euxassay_004360_21	euxassay_004360_22	euxassay_004360_23	euxassay_004360_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7322_wholemount_strong.wlz

7322_wholemount_moderate.wlz

7322_wholemount_weak.wlz

7322_wholemount_possible.wlz

7322_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7322_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 18 19 20 21 22 moderate expression: see section 08 09 17

vagus x ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 18 19 moderate expression: see section 08 17

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 15

cervical ganglion

moderate

regional

moderate expression: see section 08 16

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 15 16 17 18

not examined

regional

not examined expression: see section 01 02 03 04 22 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 04 22 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5278
Entity Detected:	Tspyl4, TSPY-like 4 ( MGI:106393)
Sequence:	sense strand is shown >T5278 CAGAGGACAAGGAAAGCTCTGCAGGGCTGTCAGCTGCCAAAATGAACGGCGTGGAAGGGAACAACGAGCT ATCTCTCGCTAACACCACGACCCCCTCCCATGCCTCCGAAGATCTGGATCTGAAGCAGGACCAGGGGCTC CAAGAGGAAACCGACACAGTGCGGGAGATGGAGGCTGCAGGTGAGGCCGGTGCGGATGGAGGCGCGTCTC CGGATTCCGAGCACTGCGGCCCCGAGCTCTGCTTCCGAGTGGCTGAGAATAGTTGTGCTGCGGCAGCGAG AGGTCTGGAGGATGCTCCGTCTCCATCAAAGGGCGGGGACGCACCCTCAGCCCCTGTGGCAGCCGACGAC AGCAGTAAAAATGGCTGTCAGCTTGAAGGGCCTCACAGCCCTGCTAAACCGAAAGCCCTGGAAGCCTGTG GTGCGGTGGGCCTGGGGTCTCAGCAGATGCCGGGGCCGAAGAAGACCAAGGAAATGACGACTACCAAGTG CGCCATCTCTGTGGCCACCGGCAAGGAGGGAGAAGCAGGAGCGGCAATGCAGGAAAAGAAGGGGTTACAG AAGGAAAAAAAGGTAGCTGGAGGAGGGAAAGAGGAGACTCGACCCAGAGCTCCGAAGATCAATTGCATGG ACTCGCTGGAGGCCATCGATCAAGAGCTGTCAAATGTAAATGCGCAGGCTGACAGGGCCTTCCTCCAGCT GGAACGCAAATTTGGGCGGATGAGAAGGCTCCACATGCAGCGCAGAAGTTTCATCATCCAAAACATCCCA GGTTTCTGGGTCACCGCTTTTCGGAACCACCCCCAGCTGTCACCAATGATTAGTGGCCAAGATGAAGACA TGATGAGGTACATGATCAATTTAGAGGTGGAGGAGCTTAAGCAGCCAAGAGTAGGGTGCAAATTTAAGTT CATCTTTCAAAGCAACCCCTACTTTCGAAATGAGGGGCTGGTCAAAGAATATGAGCGCAGATCCTCAGGT CGAGTGGTGTCACTCTCTACGCCAATCCGCTGGCACCGGGGTCAGGAACCCCAGGCCCACATCCACAGGA ATAGGGAGGGGAACACGATTCCCAGTTTCTTCAACTGGTTCTCAGACCACAGCCTCCTAGAATTCGACAG AATAGCTGAAATTATCAAAGGGGAGCTGTGGTCCAATCCCCTACAATACTATCTGATGGGCGATGGGCCA CGCCGTGGAGTTCGAGTCCCACCAAGGCAGCCAGTGGAGAGTCCCAGGTCCTTCAGGTTCCAGTCTGGCT AAGCTCTGCCCTCGTGAGAAGCTCTTGCAGAGGAGTCCTTACCACCTCCTCAGCTTGGCCAGCAGCATGC AGCCTTCTGTCTGCTTTCTCTTCCTGTTGGATTGTGTTCATTGGTTCTTCTAAGTCTCCAGTAGTTTCAA GATTGTGGCTTTCAAGTCTTTGCTCTTCTTTCTCTGGGCCATCACGATGTTCTGCATATATATATATAAT GGTGTTCCAAGTGCATGGCCTTCAAACTGCTTCTATGCCAAGTCACGTGCTGTAGTCTGTACTTCGTTCT TTGCATGGCTTGGTCCCTGTCTGTGACATGATCTTTTTTTCATTTTTTTTTTTAAGTGGTTCTCTGCCAC AAGAAAGCTTGACACATTCTTACCAAGAACTAGCCAGGCTTCATACTTTCTGCTGTGTTCCCGATATCTA TGTACTGTGAAGAACCGTGAGTTTCACCACTGCAAGATGGAACTGTACCCCAACCCAGCCATTAGCCCAA CAGGACATTCCAGGCTATTGCCAACTGATCCTAGCTGTCTTCCTGGGCCTTTGCCTTTTACCCTGCTTGT TATCTCTACAACAAGCAGGTGGCTGGTGGGTATGAGTGAAGTAACAGGCGAGGAATGTTTTCTGTCACCT CATTGGTCTTGTACCGTTGGTTGCTGTGCATTGTGATCAGCTACTGGTGAGTATGGAAGCTTGTGCAATT GCCCACTAGTTCTCTCCTGGTTGAGCTTTGCACAGGCCCAAGTGGTTCCTCAGGAACTATCTAAGGACCT AAGTTCCTAGGCAGCACACAGATACCCCCTGCTGTACCAGTTCCCATGGCTCAATTAGCTGCTGGGCAGG ATCTGTAGTCTCACGTGGCAGTGCCCAAAGCAAAGCTGAAGTGGCTCCCTTCCAGTGGTTAGTTCTGAGC CCTAACCAACAAATTATTCCTTATCCAGGCACCTTTGATAGGTAACTACTCCTCTTCCTGTGTTCACACC CACTCTGGTTAAGTCAAAGTTGCCTAGGTTTGGGTAGTTAGAAATTGTTTGTGGCCCACAGGCCTTGCTA TACCTTTTTCTCTTTGGCTCTTCTCTTTTCACCTCCCCCCAACCCCTTTTCTTTTTAAGGCAGTTACAGC CCAGAGACAGAAAAAACAAAAGACAGCAGGCTTTTTATTTGCTCTCCCCTTGGTTGTATGCAGGCTGGGA AAGGTACTGTAAAGTAGACTCCAAATCTCTCACTGCCCTGACTATTCCACCCTTCTCATAGCAAGGGAGA CCACCTCCACTCTTCCCTCCATTCTACTAGGTGCTGTGTCTTTAAGCTCTAGTTTGGGAATCTGAGAGAA GAGAAAGAAGAGTCTCAATGACAGACCCTGTGCCTGCCCTGAGCAGTCTGTGAATGAATGCACCTAGCCC ACGTTTCTAAAGGACAGCAGTTCTTTTGTGGGCCTCAGCCTGTAGAAATGACCAGAGAGCAACAATGTGC GACATCTTTGTTGTTTGCCTGATCTTGGCCCTTACCTGTCAGGTTTTGAATGTCCTTCCATGTTTCCATC CTAACAAAGAAGGAAGCAGGCTGTAGAGTGCATTGTGGGAGGGAGACCCCAAGCCTCTTCTCAGGGATGT GGGGATGTGAGGGGTCTGGATTTGCAGGACATGCTCCATTGAATTTGCAACAGCGCTGTTGTGGCTTCTG CCGCTGCAGCCCTCAGGCATTCCCTTTCTGATTGAAAGACCTTGTTGAAAGCTTCTCCAAAGCTCGTAGG TCTGGATACCTTCGTTGGAGGCTGGGAATTTTAAGAGGAGGACTAGAGGGAAGAGCCTGTTGCAGAGTGA GTTTTTCTAGGTCTGCTGAGATGATTGACACATGGAGGGAGCAGGGTTGGGGGCAGGGGTCCCTGAAGCA CATTCCAGTGCAAGGAGGTGGGGGAAGGGATCAAGTGGGTTGGGATTTAAAAAGAGACGCTTGGAGGATA TTCCTTAGTTTGTTCTAATTTTCAACTCCACCAGTAGATGTTAGTAAACAGATGTAAGTCTGAGTGAGCC TTGCCTGGGGAGATCAATGGTGTGCACTCCTCATTCCACTGCGCTCGATCCTAGTCCCCCCTGTCAGCTC ACTGGCAGGATTGATGTTTACAAGCCTGCCTTTTACTGATCATTGAGAACAAGTGCAAACATTTGAATTT CTGTCAGTTGCAGTATGAAGACTCCTGGGAACACTCACTACTAGATTAACTTGTTCTGGTGTCTCTTCAT GTATAGCTGTATTCTTAGAAGTTCAATCCGCACAATGATCTCTGAAGTCTGAAGACTTTTTGAAAACTGT TGCTATGTCTTGTAAAAGTGGTTTCCAAAAGACAAATGTAACTGTCCTACTGATATTTCTTGCTGTAGTA AGTAAGTAACACTTGGCATTATCTTCTTAACCCGTGTAAGGACTAGAACTGCTATCAACTAAACGAGGAT ATTAGTCCTTTGTCTTCTCCTGTGTGCTCAAAGGAAAGATAATGTTACTGTTATATTTCTCGTCTGCACC AAAAATAAATAAATAAATAATAAATAAATAAATAAACAAACAAATAAATAAATGTGAATAAAAAGCTCAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5361000 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5361000 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7324 same embryo
	EMAGE:7320 same embryo
	EMAGE:7321 same embryo
	EMAGE:7325 same embryo
	EMAGE:7323 same embryo
	EurExpress:euxassay_004360 same experiment
	MGI:4828974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source