EMAGE mouse gene expression spatial database

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EMAGE:10033

Rpa1 replication protein A1 ( MGI:1915525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008207. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008207_01	euxassay_008207_02	euxassay_008207_03	euxassay_008207_04

euxassay_008207_05	euxassay_008207_06	euxassay_008207_07	euxassay_008207_08	euxassay_008207_09

euxassay_008207_10	euxassay_008207_11	euxassay_008207_12	euxassay_008207_13	euxassay_008207_14

euxassay_008207_15	euxassay_008207_16	euxassay_008207_17	euxassay_008207_18	euxassay_008207_19

euxassay_008207_20	euxassay_008207_21	euxassay_008207_22	euxassay_008207_23	euxassay_008207_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10033_wholemount_strong.wlz

10033_wholemount_moderate.wlz

10033_wholemount_weak.wlz

10033_wholemount_possible.wlz

10033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 12 13 17 18 19 20 21 weak expression: see section 10 11 14 15 16

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 17 18 19 20 21 22 23 weak expression: see section 14 15 16

pons ventricular layer

strong

regional

strong expression: see section 12 13 weak expression: see section 14 15

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16 17

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T371
Entity Detected:	Rpa1, replication protein A1 ( MGI:1915525)
Sequence:	sense strand is shown >T371 CAAATACCGCATGATCTTATCGGCAAATATTGCAGATTTTCAAGAGAACCAGTGGGTGACGTGTTTCCAG GAGTCTGCAGAGGCCATCCTTGGACAGAACACTATGTACCTTGGGGAACTCAAAGAGAAGAACGAGCAGG CGTTTGAAGAGGTTTTCCAGAATGCCAACTTCCGGTCATTTACGTTCAGAATCAGGGTCAAGCTGGAGAC CTACAATGATGAATCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTTCAGAGACTATGGC AGACGGCTGATCGCGAACATCAGGAAGAACATGTGATGGGAGCAAGTACCACTGGTGGCCAGAACGGAAG AGAGAAGAACAGAAATAGAATGGCTTCCTTCCCGCCCCATGGGACAGCTCCACAATGAAGATCCTCTAGG GGAGGCTGGCAACCCATCTGGGCCCCTCTTGGGACTCACTGATAAGCAAAGACAGTGAGCTCAGAGGGC
Notes:	The probe template was PCR amplified from IMAGE:3156100 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156100 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10034 same embryo
	EMAGE:10032 same embryo
	EMAGE:10035 same embryo
	EurExpress:euxassay_008207 same experiment
	MGI:4827793 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source