EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10034

Arhgap8 Rho GTPase activating protein 8 ( MGI:1920417)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008191. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008191_01	euxassay_008191_02	euxassay_008191_03	euxassay_008191_04

euxassay_008191_05	euxassay_008191_06	euxassay_008191_07	euxassay_008191_08	euxassay_008191_09

euxassay_008191_10	euxassay_008191_11	euxassay_008191_12	euxassay_008191_13	euxassay_008191_14

euxassay_008191_15	euxassay_008191_16	euxassay_008191_17	euxassay_008191_18	euxassay_008191_19

euxassay_008191_20	euxassay_008191_21	euxassay_008191_22	euxassay_008191_23	euxassay_008191_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10034_wholemount_strong.wlz

10034_wholemount_moderate.wlz

10034_wholemount_weak.wlz

10034_wholemount_possible.wlz

10034_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10034_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

thyroid gland

weak

regional

weak expression: see section 10 11 12 16 17

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 weak expression: see section 02

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T362
Entity Detected:	Arhgap8, Rho GTPase activating protein 8 ( MGI:1920417)
Sequence:	sense strand is shown >T362 AGCAGTTTGGCGTCAGCCTGCAATACCTCAGAGACAAAAATCAAGGTGAACTCATCCCCCCGGTGCTGCG TTGGACGGTGACATATCTGAGAGAAAAAGGACTGCGCACTGAGGGCCTGTTCCGGAGATCGGCCAGCGCC CAGACTGTCCGTCAGGTGCAGCGGCTATATGATCAAGGGAAGCCCGTGAACTTTGATGATTATGGAGACA TGCACCTCCCAGCCGTGATCCTGAAGACATTTCTCCGAGAGCTGCCCCAGCCACTGCTGACCTTCCAAGC CTACGAGCAGATTCTCGGGATCACCAGTGTGGAGAGCAGCCTTCGAGTGACCCACTGCCGCCTGATCCTG CGGAGCCTCCCAGAACACAACTATGCCGTACTCCGCTACCTCATGGGCTTCCTGCATGAAGTGTCTCTGG AGAGCATTTCAAACAAGATGAACAGCTCTAACCTGGCGTGCGTGTTTGGATTGAACTTGATCTGGCCA
Notes:	The probe template was PCR amplified from IMAGE:3155759 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3155759 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10032 same embryo
	EMAGE:10035 same embryo
	EMAGE:10033 same embryo
	EurExpress:euxassay_008191 same experiment
	MGI:4823225 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source