EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10117

Trip10 thyroid hormone receptor interactor 10 ( MGI:2146901)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000162. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000162_01	euxassay_000162_02	euxassay_000162_03	euxassay_000162_04

euxassay_000162_05	euxassay_000162_06	euxassay_000162_07	euxassay_000162_08	euxassay_000162_09

euxassay_000162_10	euxassay_000162_11	euxassay_000162_12	euxassay_000162_13	euxassay_000162_14

euxassay_000162_15	euxassay_000162_16	euxassay_000162_17	euxassay_000162_18	euxassay_000162_19

euxassay_000162_20	euxassay_000162_21	euxassay_000162_22	euxassay_000162_23	euxassay_000162_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10117_wholemount_strong.wlz

10117_wholemount_moderate.wlz

10117_wholemount_weak.wlz

10117_wholemount_possible.wlz

10117_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10117_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 08

otic capsule

moderate

homogeneous

moderate expression: see section 02 03 04 08

inner ear vestibular component

moderate

regional

moderate expression: see section 08

axial skeleton

moderate

homogeneous

moderate expression: see section 10

cranium

strong

homogeneous

strong expression: see section 02 moderate expression: see section 01

basioccipital bone

strong

regional

strong expression: see section 02 moderate expression: see section 06 08 17 18 19 20 23 24

basisphenoid bone

moderate

regional

moderate expression: see section 03 04 05 08 12 13 14 15 16 17 22 23 24 not examined expression: see section 02

exoccipital bone

moderate

homogeneous

moderate expression: see section 04

foramen ovale of chondrocranium

moderate

homogeneous

moderate expression: see section 04 05 06 20

foramen rotundum

moderate

homogeneous

moderate expression: see section 05 18

sphenoid bone

moderate

homogeneous

moderate expression: see section 05 06 07 09 12

vault of skull

moderate

homogeneous

moderate expression: see section 01 not examined expression: see section 02

frontal bone primordium

strong

homogeneous

strong expression: see section 02 moderate expression: see section 03 06 09 17 22 24

inter-parietal bone primordium

moderate

regional

moderate expression: see section 21 22

temporal bone

moderate

regional

moderate expression: see section 24

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 09 10 20 21

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

moderate

spotted

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1561
Entity Detected:	Trip10, thyroid hormone receptor interactor 10 ( MGI:2146901)
Sequence:	sense strand is shown >T1561 GCCCCGCATTGCAGAGACCCTGGGCAACATTGAGAGGCTGAAGTTGGAAGTGCAGAAGTATGAGGCTTGG TTGGCAGAAGCTGAAAGCCGGGTCCTCAGTAACCGAGGGGACAGCCTAAGCCGTCACGCTAGGCCCCCTG ATCCCCCAACTACTGCCCCACCTGATAGCAGCAGTAGCAGCACCAACAGTGGATCCCAGGACAATAAGGA GAGCTCAGAAGAGCCCCCTTCAGAAGGCCAGGACACCCCCATCTATACTGAGTTCGATGAGGACTTTGAG GAGCCTGCATCCCCTATCGGCCAGTGTGTGGCTATCTACCATTTTGAAGGATCCAGCGAGGGAACCGTCT CCATGTCCGAGGGGGAAGACCTCAGCCTGATGGAGGAAGACAAGGGTGATGGATGGACGCGGGTCAGGAG GAAACAGGGAGCTGAGGGCTACGTGCCCACCTCTTACCTCCGAGTCACACTCAACTGAACCCCACCAGAG GGGGACGAGGGGCAGGGCTGTCAGCTGCTGCTT
Notes:	The probe template was PCR amplified from IMAGE:907193 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:907193 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10120 same embryo
	EMAGE:10122 same embryo
	EMAGE:10119 same embryo
	EMAGE:10121 same embryo
	EMAGE:10118 same embryo
	EurExpress:euxassay_000162 same experiment
	MGI:4828923 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source