EMAGE mouse gene expression spatial database

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EMAGE:10122

Hat1 histone aminotransferase 1 ( MGI:96013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000115_01	euxassay_000115_02	euxassay_000115_03	euxassay_000115_04

euxassay_000115_05	euxassay_000115_06	euxassay_000115_07	euxassay_000115_08	euxassay_000115_09

euxassay_000115_10	euxassay_000115_11	euxassay_000115_12	euxassay_000115_13	euxassay_000115_14

euxassay_000115_15	euxassay_000115_16	euxassay_000115_17	euxassay_000115_18	euxassay_000115_19

euxassay_000115_20	euxassay_000115_21	euxassay_000115_22	euxassay_000115_23	euxassay_000115_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10122_wholemount_strong.wlz

10122_wholemount_moderate.wlz

10122_wholemount_weak.wlz

10122_wholemount_possible.wlz

10122_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10122_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 weak expression: see section 12

brain

strong

regional

strong expression: see section 14 17 18 19 20 21 22 23

forebrain

strong

regional

strong expression: see section 14 15 16

diencephalon

strong

regional

strong expression: see section 12 13 15

telencephalon

strong

graded

strong expression: see section 07 09 12 13

cerebral cortex

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 08 09 10 11

hindbrain

strong

homogeneous

strong expression: see section 01 03 05 06 14 15 16 moderate expression: see section 04

midbrain

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14

optic ii nerve

moderate

regional

moderate expression: see section 01

optic stalk

moderate

regional

moderate expression: see section 01

liver

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

testis

moderate

regional

moderate expression: see section 05 06 07 08 09

testis

moderate

regional

moderate expression: see section 21 22 23 24

lung

moderate

spotted

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1562
Entity Detected:	Hat1, histone aminotransferase 1 ( MGI:96013)
Sequence:	sense strand is shown >T1562 TTTTTTTTTTTTTTATTTCACAAGCCATCTTTTATTAATGCAATATATGTTCATAAACTCTAGACAGTAT TTTAAAATATACAATCTCTCAAAAGGAAATATTCCACTAAGGAGAACATAGATAACTTTAGGGTCATCAG AGGTTCACAGCACGATGCACAGAAATCTGCAGGTATTCTGGGGAACAAGGGCTTCACTCTTGAGCAAGTC GCTCAATGACACGCCGGTAGTCCTCCACCAGCTCCTGAAATCTCTCTTCCAGCTGCTCATGCTGCACACT TATTTCTATCTGGTTCATCTGATTTGTCAGCTCTTCTGGTCTCAGGCATTTCTTCATCTTGGCAAGATCC CTCTGCTTTTTCTTGTAAGGGCTAATTAGTCTTCTTTTAATATCCAGTCTGTAGCTTCTGTATTGCTCAG CATCGCTCATGTCAGTCACCAGCAGTCGAAGAATTTCATAAACCCTTCTAGCATGTTGCTTAT
Notes:	The probe template was PCR amplified from IMAGE:907313 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:907313 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10120 same embryo
	EMAGE:10119 same embryo
	EMAGE:10117 same embryo
	EMAGE:10121 same embryo
	EMAGE:10118 same embryo
	EurExpress:euxassay_000115 same experiment
	MGI:4825324 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source