EMAGE mouse gene expression spatial database

EMAGE:10121

Zfp54 zinc finger protein 54 ( MGI:99201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008234_01

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

frenulum

strong

regional

strong expression: see section 01

urethra of male

strong

regional

strong expression: see section 01

lung

strong

regional

strong expression: see section 01

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1565
Entity Detected:	Zfp54, zinc finger protein 54 ( MGI:99201)
Sequence:	sense strand is shown >T1565 TTTTTTTTTTTTTTCATATAATCACATTTGGAATTTATTTTGACAAACTTTGTGATAAATTGTAAGATTT AAAACTTTATTAAAGGCTTTCCCCTGTCTCTCATGCATATATTTCTATTATGTATAATGTTGGGATTTGA ACACAGAGAGAATACTGGATGAATGCTATGTCACATTATTTACAATTTTATGTTTCTCTCCAGTATGAAC TTTCTGATGTCTTTTAAGATGTGAGTATTGGATATAGGATCTGTCACATTCCATACATTTGTAAGGTTTT TCTCCAGTATGAATTTTCTGATGTGCTCTAAGAATTGCACATGTAGTAAGTGATTTGCCACATTCCTTAC ATACATAAGGTTTCTCTCCAGTATGAACTCTCTGATGTGTTCTAAGTTGTGAGGCCTGGGTAAATGATTT GTCACATTCACTACATTTGTAAGGTTTTTCTCCAGTATGAATTCTCTGATGTACTCTAAGAGTTGAG
Notes:	The probe template was PCR amplified from IMAGE:907451 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:907451 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10120 same embryo
	EMAGE:10122 same embryo
	EMAGE:10119 same embryo
	EMAGE:10117 same embryo
	EMAGE:10118 same embryo
	EurExpress:euxassay_008234 same experiment
	MGI:4829318 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source