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EMAGE:10524

2310003F16Rik RIKEN cDNA 2310003F16 gene ( MGI:1914943)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524
"Pseudo-wholemount" of euxassay_000792. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000792_01 euxassay_000792_02 euxassay_000792_03 euxassay_000792_04
EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524
euxassay_000792_05 euxassay_000792_06 euxassay_000792_07 euxassay_000792_08 euxassay_000792_09
EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524
euxassay_000792_10 euxassay_000792_11 euxassay_000792_12 euxassay_000792_13 euxassay_000792_14
EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524
euxassay_000792_15 euxassay_000792_16 euxassay_000792_17 euxassay_000792_18 euxassay_000792_19
EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524 EMAGE:10524
euxassay_000792_20 euxassay_000792_21 euxassay_000792_22 euxassay_000792_23 euxassay_000792_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10524Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10524_wholemount_strong.wlz
10524_wholemount_moderate.wlz
10524_wholemount_weak.wlz
10524_wholemount_possible.wlz
10524_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10524_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3896
Entity Detected:2310003F16Rik, RIKEN cDNA 2310003F16 gene ( MGI:1914943)
Sequence:sense strand is shown

>T3896
CCCGCGTCCGGCGGCAGCCTATGCGGCGGCGCGGTGAGATCGAAATGGCGACCGAGGGAGACGTGGAGTT
GGAGTTAGAGACCGAGACCAGCGGCCCGGAGCGGCCTCCCGAGAAGCCAAGGAAACATGACAGTGGTGCT
GCTGACCTAGAGCGGGTTACTGACTATGCGGAGGAGAAGGAGATCCAGAGTTCGAATCTTGAAACGGCTA
TGTCCGTCATTGGAGACAGACGGTCCAGGGAACAAAAGGCCAAACAAGAGCGGGAGAAGGAACTGGCGAA
GGTCACTATCAAGAAGGAAGATCTGGAGTTGATAATGACGGAAATGGAGATCTCTCGAGCAGCAGCAGAA
AGGAGCTTGAGGGAACACATGGGCAATGTGGTGGAGGCTCTTATTGCCCTAACCAACTGATAATGTGCTT
TTTTAGACATTCTGGACTGACTGACTAACTGAAATAAAGTGTTCTGTTTTGCTGTTTTTTTTTCTTTAGT
TTCATCTCAGATCAAATGCATTATATATTCTATTTTATTGTCAGTTTCTATGTTGGACCTTGATGAAACT
GTGTGAACAATCAGTATAAAGAAGTACCTAATGTTCAAATGCAGGCTCTTATATTTTGTGTGTGATTCCT
CTACTGCCATAGTGTTTCTAATAACTACCACATGGGAAACGAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5028838 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5028838 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10526 same embryo
 EMAGE:10523 same embryo
 EMAGE:10525 same embryo
 EMAGE:10528 same embryo
 EMAGE:10527 same embryo
 EurExpress:euxassay_000792 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS