EMAGE mouse gene expression spatial database

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EMAGE:10526

Ikbkg inhibitor of kappaB kinase gamma ( MGI:1338074)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000801. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000801_01	euxassay_000801_02	euxassay_000801_03	euxassay_000801_04

euxassay_000801_05	euxassay_000801_06	euxassay_000801_07	euxassay_000801_08	euxassay_000801_09

euxassay_000801_10	euxassay_000801_11	euxassay_000801_12	euxassay_000801_13	euxassay_000801_14

euxassay_000801_15	euxassay_000801_16	euxassay_000801_17	euxassay_000801_18	euxassay_000801_19

euxassay_000801_20	euxassay_000801_21	euxassay_000801_22	euxassay_000801_23	euxassay_000801_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10526_wholemount_strong.wlz

10526_wholemount_moderate.wlz

10526_wholemount_weak.wlz

10526_wholemount_possible.wlz

10526_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10526_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3897
Entity Detected:	Ikbkg, inhibitor of kappaB kinase gamma ( MGI:1338074)
Sequence:	sense strand is shown >T3897 CCACGCGTCCGAAGACATAGAACCTGCTCTCCTTGTTTTGGCTCAGCCTGCAACTGACAGTCTGCCTGGC TTCAAGTCACCCAAGGGAGGTGAGGAGGAAGAAGTGCTACTGGGTGGTGCTTTAGCACCTGTGATGGTCC AAGTCTGGTCTCCACTCAATGGCTCAGCACAGAGTTCTGAGATGTGACACAAGTACATGTATATCAGGTA CTGCTGTGATGATCAGGACACACAGACACTGTCCTGTTGGATGAACAAGCACCCCTGGAAGAACCAGCTG AGTGAGATGGTGCAGCCCAGTGGTGGCCCAGCAGAGGACCAGGACATGCTGGGTGAAGAATCTTCTCTGG GGAAGCCTGCAATGCTACATCTGCCTTCAGAGCAGGGTACTCCTGAGACCCTCCAGCGCTGCCTGGAAGA GAATCAAGAGCTCCGAGACGCTATCCGGCAGAGCAATCAGATGCTGAGGGAACGCTGTGAGGAGCTGCTG CATTTCCAGGTCAGCCAGCGGGAGGAGAAGGAGTTCCTTATGTGCAAATTCCAGGAAGCCCGGAAGCTGG TGGAGAGACTGAGCTTGGAGAAGCTTGATCTTCGGAGTCAGAGGGAACAGGCCTTAAAGGAGTTGGAGCA ACTGAAGAAATGCCAACAGATGGCTGAGGACAAGGCCTCTGTGAAAGCTCAGGTGACATCATTGCTCGGA GAACTCCAGGAGAGCCAGAGCCGTTTGGAGGCTGCCACCAAGGATCGGCAAGCTTTAGAGGGAAGGATTC GAGCAGTTAGTGAGCAGGTCAGACAGCTGGAGAGTGAGCGGGAGGTGCTACAGCAGCAGCACAGCGTCCA GGTGGACCAGCTGCGTATGCAGAACCAGAGCGTGGAGGCTGCCTTGCGAATGGAGCGGCAGGCTGCTTCA GAGGAGAAGCGGAAGCTGGCTCAGTTGCAGGCAGCCTATCACCAGCTCTTCCAAGACTACGACAGCCACA TTAAGAGCAGCAAGGGCATGCAGCTGGAAGATCTGAGGCAACAGCTCCAGCAAGCTGAGGAGGCCCTGGT AGCCAAACAGGAATTGATTGATAAGCTGAAAGAGGAGGCTGAGCAGCACAAGATTGTGATGGAGACTGTG CCAGTCTTGAAGGCCCAGGCGGATATCTACAAGGCTGACTTCCAAGCTGAGAGGCATGCCCGGGAGAAGC TGGTGGAGAAGAAGGAGTATTTGCAGGAGCAGCTGGAGCAGCTGCAGCGCGAGTTCAACAAGCTGAAAGT TGGCTGCCATGAGTCAGCCAGGATTGAGGATATGAGGAAGCGGCATGTAGAGACTCCCCAGCCTCCTTTA CTCCCTGCTCCAGCTCACCACTCCTTTCATTTGGCCTTGTCCAACCAGCGGAGGAGCCCTCCTGAAGAAC CTCCTGACTTCTGTTGTCCGAAGTGCCAGTATCAGGCTCCTGATATGGACACTCTACAGATACATGTCAT GGAGTGCATAGAGTAGGAGCAGCAGATGCAAGGCCACTTGCAGTACTATGTCCTGATCTGTGTGACTTGT GCTTTCCTGTTTTACCTGCATAGTCCACACTTAAGGGCTTGCTTTAGCCCTTTGGTCCCCCATTTAGGGT AGACAGCCCCATTCAGGGCTTTTTTTTTTCTGTGTGCCTGATCCAGTTTGCCTCTGGTGGCTTCTTCCCT CTTCTCCCATAGTCCTAGGGAGTCTAGAATGGAGGCCAGGGGCTCTTAGGGAGCATCCCTTCTCCAAGCA GGTCTGGGTACAGCTTTTCTTCTCTCCAACTGGTACCTTTCTTGCCGGTGAACTGCAGGCTCTCCTCCCA GGGCATGTGGCACTTGGGTCTATAACATGTGTTACCTCTGGTAGACATGTGGAAAGTATTCTGTCCTTTT GTTACTGTAATTAATGGTGTAGTGAAAGTACTTGTACACTGATCTGTGTGTACCTTTAGGACAGATGCTT AGATGTGACATTGGATCCTTGCCCTGGAGGAGGTGGGCAGACCTTTGGATTTTTGCCATCTCTAAGATAT ACCATTCTTTACTAAAGAATTTGCTTAAGTGTCTTTTTCTGGGGCATGTGGGCCCTTTTTAATTCCTCTG ATGTAACTTGGATTTTCATAATTCCTACCCATTTTTCTGTTGGGACATTGCCATTTGTTCTTGGGCTTTG TAGAGCTTTTTGATTATCAGTCAGGTTGGTTTTGACTATTACAGAAGTGCACCCCTTGTTCCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030381 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030381 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10524 same embryo
	EMAGE:10523 same embryo
	EMAGE:10525 same embryo
	EMAGE:10528 same embryo
	EMAGE:10527 same embryo
	EurExpress:euxassay_000801 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source