EMAGE mouse gene expression spatial database

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EMAGE:10586

Slc22a4 solute carrier family 22 (organic cation transporter), member 4 ( MGI:1353479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000828. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000828_01	euxassay_000828_02	euxassay_000828_03	euxassay_000828_04

euxassay_000828_05	euxassay_000828_06	euxassay_000828_07	euxassay_000828_08	euxassay_000828_09

euxassay_000828_10	euxassay_000828_11	euxassay_000828_12	euxassay_000828_13	euxassay_000828_14

euxassay_000828_15	euxassay_000828_16	euxassay_000828_17	euxassay_000828_18	euxassay_000828_19

euxassay_000828_20	euxassay_000828_21	euxassay_000828_22	euxassay_000828_23	euxassay_000828_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10586_wholemount_strong.wlz

10586_wholemount_moderate.wlz

10586_wholemount_weak.wlz

10586_wholemount_possible.wlz

10586_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10586_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

homogeneous

strong expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3940
Entity Detected:	Slc22a4, solute carrier family 22 (organic cation transporter), member 4 ( MGI:1353479)
Sequence:	sense strand is shown >T3940 CCACGGCGTGTTTGCACGCAGCCACCTAGGAAGATCCCCTCAGCGCGCCGCAATCGCTGAATCCTTTCTC TCCACCCACCTCCCTCACGCAAGCTGAGGAGGAGAGGTGGAAACATGCGGGACTACGACGAGGTGATCGC CTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTCATCTTCTTTCTGCTCAGCGCCAGCATCATCCCCAAT GGCTTCAATGGTATGTCAGTCGTGTTCCTGGCGGGGACCCCCGAGCACCGTTGCCTGGTTCCTGACACTG TGAACCTGAGCAGCTCCTGGCGCAACCACAGCATCCCCTTGGAGACGAAGGACGGACGACAGGTGCCTCA GAGCTGCCGCCGCTACCGACTGGCCACCATCGCCAACTTCTCTGCGATGGGGCTGGAGCCAGGACAGGAC GTGGATCTGGAGCAGCTGGAGCAGGAGAGCTGCCTGGATGGCTGGGAGTACGACAAGGACATCTTCCTGT CCACCATCGTGACAGAGTGGAATCTGGTGTGTGAGGATGACTGGAAGACACCCCTCACCACCTCCCTGTT CTTCGTAGGCGTTCTCTGCGGCTCCTTCGTGTCTGGGCAGCTGTCAGACAGGTTTGGCAGGAAGAAAGTC CTCTTTGCAACCATGGCTGTGCAGACTGGATTCAGCTTCGTGCAGATTTTCTCAACCAACTGGGAGATGT TCACTGTGTTGTTTGCCATTGTGGGCATGGGCCAGATCTCCAACTACGTGGTGGCCTTCATACTAGGAAC TGAAATCCTGAGCAAGTCGGTTCGCATCATCTTCTCCACATTAGGAGTCTGTACATTTTTTGCAATCGGC TACATGGTCCTGCCGCTGTTTGCATACTTCATCAGAGACTGGAGGATGCTGCTGCTGGCCCTGACACTGC CTGGCCTGTTCTGTGTTCCCCTGTGGTGGTTTATTCCAGAATCTCCCCGGTGGCTGATATCCCAGAGGAG ATTTGCAGAGGCCGAACAGATCATCCAGAAAGCCGCAAAGATGAACAGCATCGTGGCGCCAGCAGGGATA TTCGATCCTCTAGAGCTACAGGAGCTAAACTCCTTGAAGCAGCAGAAAGTCATAATCCTGGACCTGTTCA GGACTCGGAACATTGCCACCATAACCGTGATGGCTGTGATGCTGTGGATGCTAACCTCAGTGGGTTACTT TGCTCTGTCTCTCAATGTTCCTAATTTACATGGAGATGTCTACCTGAACTGCTTCCTCTCTGGCCTGATT GAAGTTCCAGCTTACTTCACAGCCTGGCTGCTACTGCGAACCCTGCCACGGAGATATATTATAGCTGGGG TGCTATTCTGGGGAGGAGGTGTGCTTCTCTTGATCCAAGTGGTACCTGAAGATTATAACTTTGTGTCCAT TGGACTGGTGATGCTGGGGAAATTTGGGATCACCTCTGCCTTCTCCATGTTGTATGTCTTCACTGCGGAG CTCTACCCAACCCTGGTCAGGAACATGGCTGTGGGCATCACCTCCATGGCCTCTCGGGTGGGCAGCATCA TTGCCCCCTATTTCGTTTACCTGGGCGCCTATAACAGACTCCTACCCTACATCCTCATGGGCAGTCTGAC TGTCCTCATTGGAATCATCACGCTTTTTTTCCCTGAAAGTTTTGGAGTGACTCTACCAGAGAACTTGGAG CAGATGCAGAAAGTGAGAGGGTTCAGATGTGGGAAAAAATCAACAGTCTCAGTGGACAGAGAAGAAAGCC CCAAGGTTCTAATAACTGCATTCTAACGAGGTTTCCAAGGCACTTGGCAAACTGAAAAGCAGATGTATAC AATGAGCAGGGTGTGATAGAGCAAGCCTGCAATCCCAGCGCTCTTGGGGTGGAGACAGAAGATCAGGAGT TCAAGGTCATCCTTGGCTACAGCAGGAGTGTAAGACCAGCCTGTCTTACCACAAGCAACCCTGTCTCAAC AGAACAAATCAAAAGCCTTTTCTGCTGAAAGGGATTAACAGAAACAATGAGCACCAAACTGGACTTGTGG AGAAATGCACACTATCTCATGAATTCTGGGCCACTCTTCCAGATGGCCTTGTTTTCAAGAACAAACATTT CTAAATAGTCTTGACTACTAACTATTCAATGAAATTGATTTGTAAGATTTTTTTTTTTTTTTTTTTTGGA AAATGTGTTAGTCAAGGACTGGCAAAATCCATATGAAGATTAACACACATTTCTAAACGTATAAATGCTA TCCAAATAAAATTATCCTAACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3670138 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3670138 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10582 same embryo
	EMAGE:10584 same embryo
	EMAGE:10581 same embryo
	EMAGE:10583 same embryo
	EMAGE:10585 same embryo
	EurExpress:euxassay_000828 same experiment
	MGI:4828138 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source