EMAGE mouse gene expression spatial database

EMAGE:10683

Daxx Fas death domain-associated protein ( MGI:1197015)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003265. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003265_01	euxassay_003265_02	euxassay_003265_03	euxassay_003265_04

euxassay_003265_05	euxassay_003265_06	euxassay_003265_07	euxassay_003265_08	euxassay_003265_09

euxassay_003265_10	euxassay_003265_11	euxassay_003265_12	euxassay_003265_13	euxassay_003265_14

euxassay_003265_15	euxassay_003265_16	euxassay_003265_17	euxassay_003265_18	euxassay_003265_19

euxassay_003265_20	euxassay_003265_21	euxassay_003265_22	euxassay_003265_23	euxassay_003265_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T345
Entity Detected:	Daxx, Fas death domain-associated protein ( MGI:1197015)
Sequence:	sense strand is shown >T345 TTGTGATCCGCGCTCAGGGGATCCTCGAGAGACCGTTTCTGAGGGGAATTTGAACCCCCATGGCCACCGA TGACAGCATCATTGTACTTGATGATGACGATGAAGATGAAGCTGCTGCTCAACCAGGGCCCTCCAACCTA CCCCCCAATCCTGCCTCAACAGGACCTGGTCCTGGCCTGTCTCAACAGGCCACTGGTCTCTCTGAGCCCC GCGTGGATGGAGGGAGCAGTAACTCCGGTAGTAGGAAGTGCTACAAGTTGGATAATGAGAAGCTCTTTGA AGAGTTCCTTGAACTGTGTAAGACGGAGACATCAGACCACCCTGAGGTGGTTCCGTTCCTCCACAAACTG CAGCAGCGTGCCCAGTCTGTGTTTCTGGCCTCTGCAGAGTTCTGCAACATCCTCTCCAGGGTTCTGGCTC GGTCTCGGAAGCGGCCCGCTAAGATCTATGTGTACATTAACGAGCTCTGCACTGTTCTTAAAGCTCACTC CATCAAGAAGAAG
Notes:	The probe template was PCR amplified from IMAGE:3154888 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3154888 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10687 same embryo
	EMAGE:10684 same embryo
	EMAGE:10686 same embryo
	EMAGE:10685 same embryo
	EMAGE:10682 same embryo
	EurExpress:euxassay_003265 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source