EMAGE mouse gene expression spatial database

EMAGE:10686

Cfi complement component factor i ( MGI:105937)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003263. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003263_01	euxassay_003263_02	euxassay_003263_03	euxassay_003263_04

euxassay_003263_05	euxassay_003263_06	euxassay_003263_07	euxassay_003263_08	euxassay_003263_09

euxassay_003263_10	euxassay_003263_11	euxassay_003263_12	euxassay_003263_13	euxassay_003263_14

euxassay_003263_15	euxassay_003263_16	euxassay_003263_17	euxassay_003263_18	euxassay_003263_19

euxassay_003263_20	euxassay_003263_21	euxassay_003263_22	euxassay_003263_23

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T189
Entity Detected:	Cfi, complement component factor i ( MGI:105937)
Sequence:	sense strand is shown >T189 TTGGCCCCGCACTCCTCCGGCATGAAGCTCGCTCATCTCAGTCTCTTTCTCTTGGCTCTCCACTTGAGTT CTTCCAGGTCCCCGTCAGCATCAGATCTGCCTCAGGAGGAGCTGGTCGATCAGAAATGCTTACTGCAGAA ATACACGCATCGCTCCTGTAACAAGGTCTTCTGCCAGCCGTGGCAGAGATGTATCGAAGGCACCTGTATC TGCAAACTCCCCTACCAGTGCCCAAGGGCTGGCACCCCAGTGTGTGCCATGAATGGAAGGAGCTACCCGA CATACTGTCACCAGAAGAGTTTCGAATGTCTTCACCCAGAGATCAAGTTTTCACATAATGGAACGTGCGC AGCTGAAGGAAAGTTTAATGTTTCCTTAATTTATGGGAGAACAAAAACAGAGGGACTTGTTCAAGTCAAA CTTGTGGACCAAGATGAGAGAATGTTCATATGTAAAAACAGCTGGAGCATGGCGGA
Notes:	The probe template was PCR amplified from IMAGE:2647510 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2647510 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10687 same embryo
	EMAGE:10684 same embryo
	EMAGE:10685 same embryo
	EMAGE:10683 same embryo
	EMAGE:10682 same embryo
	EurExpress:euxassay_003263 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source