EMAGE mouse gene expression spatial database

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EMAGE:10763

Klf7 Kruppel-like factor 7 (ubiquitous) ( MGI:1935151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003485. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003485_01	euxassay_003485_02	euxassay_003485_03	euxassay_003485_04

euxassay_003485_05	euxassay_003485_06	euxassay_003485_07	euxassay_003485_08	euxassay_003485_09

euxassay_003485_10	euxassay_003485_11	euxassay_003485_12	euxassay_003485_13	euxassay_003485_14

euxassay_003485_15	euxassay_003485_16	euxassay_003485_17	euxassay_003485_18	euxassay_003485_19

euxassay_003485_20	euxassay_003485_21	euxassay_003485_22	euxassay_003485_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10763_wholemount_strong.wlz

10763_wholemount_moderate.wlz

10763_wholemount_weak.wlz

10763_wholemount_possible.wlz

10763_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10763_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 15 16

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 18 19

ventral grey horn

moderate

regional

moderate expression: see section 17 weak expression: see section 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 15 16

cervical ganglion

moderate

regional

moderate expression: see section 10 16

thoracic ganglion

moderate

regional

moderate expression: see section 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18

not examined

homogeneous

not examined expression: see section 02 03 04 22 23

neural retina

strong

regional

strong expression: see section 02 03 04 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 15 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6683
Entity Detected:	Klf7, Kruppel-like factor 7 (ubiquitous) ( MGI:1935151)
Sequence:	sense strand is shown >T6683 GTCAGTGAGTTTGCGTTCCCCTTCCTGATCCACGCGCTGGAGTGATTAGAGCCCTGGAAGGGAATTGTTA CTCCCGTGAAGTCCCCTTTTCCTGGCAGTCATCTGCACTGTACACGCTGGATCCCTCTCTCCGATCCACC CCACTCACTCGCTCCTCTCTCACCTCCTCTCTCACCTCCTCTCTCTCCCTCCTCTCTCACCTCCTCTCTC CCTCTCTCCTGCATTGATTTTTTTTCTCTCCTTTTTTTAGTTGACTGAAACAAAACAAAACAAAAGGGCC ACTGGATGTCTGCCTTCTTGGGGGGTGAGCCAGACAGACTGACAAACAAACAGACCCAGCTGAGCTCGGG GGAGGGTTTCTCCTCCGGTTTTGCCCGGCAGCAGCAGCATGGACGTGTTGGCTAGTTATAGTATATTCCA GGAGCTACAACTTGTCCACGACACCGGCTACTTCTCAGCTTTGCCATCCCTGGAGGAGACCTGGCAGCAG ACATGCCTTGAGTTGGAACGCTACCTCCAGACAGAACCCCGGCGGATCTCGGAGACCTTTGGTGAGGACT TGGACTGCTTCCTCCACGCTTCTCCTCCCCCATGCATTGAGGAGAGCTTCCGGCGCCTAGACCCCTTGCT GCTCCCAGTGGAAGCCACCATCTGCGAGAAGAGCTCCGCAGTGGACATTTTGCTCTCTCGGGACAAGTTG CTATCTGAGACCTGCCTCAGCCTCCAGCCAACCAGCTCTTCTCTAGACAGCTACACAGCCGTCAACCAGG CCCAGCTCAACGCAGTGACCTCATTAACGCCGCCCTCATCCCCTGAGCTCAGCCGCCATCTGGTCAAAAC CTCACAGACTCTCTCAGCCGTGGATGGCACAGTGACGTTGAAACTGGTGGCCAAGAAGGCTTCCCTCAGC TCGGTGAAGGTGGGAGGGGTGGCGGCGGCGGCAGCCGTGACACCAGCTGGAGCAGTTAAGAGTGGACAGA GCGACAGTGAGCAAGGAGGGGGAGGGGCTGACACATGCCCTGAAAACAAGAAGAGGGTCCATCGCTGTCA GTTTAACGGGTGCCGGAAAGTTTATACAAAAAGCTCCCACTTAAAGGCCCACCAGAGGACTCACACAGGT GAGAAGCCTTACAAGTGCTCATGGGAAGGATGCGAGTGGCGTTTTGCACGGAGCGATGAGCTCACAAGGC ACTACAGGAAACACACAGGTGCAAAGCCCTTTAAGTGTAACCACTGCGACAGGTGTTTCTCCAGGTCTGA CCATCTTGCGCTCCATATGAAGAGACATATCTAAACAAAACAAAACAAGACAAAATAAAAACAAACAAAA GTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10762 same embryo
	EMAGE:10764 same embryo
	EMAGE:10761 same embryo
	EurExpress:euxassay_003485 same experiment
	MGI:4825786 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source