EMAGE mouse gene expression spatial database

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EMAGE:10796

Syn1 synapsin I ( MGI:98460)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003231. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003231_01	euxassay_003231_02	euxassay_003231_03	euxassay_003231_04

euxassay_003231_05	euxassay_003231_06	euxassay_003231_07	euxassay_003231_08	euxassay_003231_09

euxassay_003231_10	euxassay_003231_11	euxassay_003231_12	euxassay_003231_13	euxassay_003231_14

euxassay_003231_15	euxassay_003231_16	euxassay_003231_17	euxassay_003231_18	euxassay_003231_19

euxassay_003231_20	euxassay_003231_21	euxassay_003231_22	euxassay_003231_23	euxassay_003231_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10796_wholemount_strong.wlz

10796_wholemount_moderate.wlz

10796_wholemount_weak.wlz

10796_wholemount_possible.wlz

10796_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10796_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19 20

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 12 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 15 16

dorsal root ganglion

strong

regional

strong expression: see section 10 11 12 13 14 18 19 20 21

not examined

homogeneous

not examined expression: see section 01 02 03 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17

lower jaw molar

strong

regional

strong expression: see section 07 20

upper jaw molar

strong

regional

strong expression: see section 06 07 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5422
Entity Detected:	Syn1, synapsin I ( MGI:98460)
Sequence:	sense strand is shown >T5422 AGTCGCGTCGTGCCAGAGAGCACCGCCGTGCTCCTGAGCCCCTCGCGCTCCGCCCCCGCGGCCCACAGAC CCACTGCCCCTTGGATCCCCTGCCCCACGCCGCCACCATGAACTACCTGCGGCGCCGCCTGTCGGACAGC AACTTCATGGCCAATCTGCCGAATGGGTACATGACAGACCTGCAGCGCCCGCAACCGCCCCCGCCGCCTC CCTCGGCCGCCAGCCCTGGGGCCACGCCCGGCTCCGCGACAGCCTCTGCCGAGAGGGCCTCCACAGCTGC TCCAGTGGCTTCTCCAGCAGCCCCTAGTCCTGGGTCCTCGGGGGGCGGCGGCTTCTTCTCGTCGCTGTCT AACGCGGTCAAGCAAACCACAGCAGCCGCAGCCGCCACCTTCAGCGAGCAGGTGGGCGGTGGCTCTGGGG GCGCAGGCCGCGGGGGCGCCGCCGCCAGGGTGCTGCTGGTCATCGACGAACCGCACACCGACTGGGCAAA ATACTTCAAAGGGAAGAAGATCCATGGAGAAATTGACATTAAAGTAGAGCAAGCTGAATTCTCTGATCTC AATCTTGTGGCTCATGCCAATGGTGGATTCTCTGTGGACATGGAAGTTCTTCGGAATGGAGTCAAAGTTG TGAGGTCTCTGAAGCCAGACTTTGTGCTGATCCGCCAGCATGCCTTCAGCATGGCACGTAATGGAGACTA CCGAAGTTTGGTCATTGGGCTGCAGTATGCTGGAATCCCCAGTGTAAACTCTTTGCATTCTGTCTACAAC TTCTGTGACAAACCCTGGGTGTTTGCCCAGATGGTTCGACTACACAAGAAGCTTGGAACAGAGGAATTCC CTCTGATTGATCAGACTTTCTATCCTAATCACAAAGAGATGCTCAGCAGCACAACATACCCTGTGGTTGT GAAGATGGGCCACGCACATTCTGGGATGGGCAAGGTCAAGGTAGACAACCAACATGACTTCCAGGATATT GCAAGTGTTGTGGCACTGACTAAGACATATGCCACTGCTGAGCCCTTCATTGATGCTAAATATGATGTGC GTGTCCAGAAGATTGGGCAGAACTACAAGGCCTACATGAGGACATCAGTGTCGGGTAACTGGAAGACCAA TACAGGTTCTGCTATGCTTGAGCAGATTGCCATGTCTGACAGGTACAAGTTGTGGGTAGACACGTGCTCA GAGATTTTTGGGGGACTTGACATCTGCGCAGTGGAAGCGCTGCATGGCAAGGACGGAAGGGATCACATTA TTGAGGTGGTGGGCTCCTCCATGCCACTCATTGGTGATCACCAGGATGAAGACAAGCAGCTCATCGTGGA ACTTGTGGTCAACAAGATGACTCAGGCTCTGCCTCGGCAGCCGCAGCGGGATGCTTCCCCTGGCAGGGGC TCCCACAGCCAGTCTTCATCCCCAGGAGCCCTGACCTTGGGCCGCCAGACCTCCCAGCAGCCTGCAGGTC CTCCTGCTCAACAACGACCCCCACCCCAGGGAGGCCCTCCACAGCCAGGCCCAGGACCTCAGCGCCAGGG ACCCCCGCTGCAGCAGCGCCCACCCCCACAAGGCCAGCAACATCTTTCTGGCCTTGGACCGCCAGCTGGC AGCCCTCTGCCTCAGCGCCTACCAAGTCCCACCGCAGCACCTCAGCAGTCTGCCTCTCAGGCCACACCAG TGACCCAGGGTCAAGGCCGCCAGTCGCGGCCAGTGGCAGGAGGCCCTGGAGCACCTCCAGCAGCGCGCCC ACCAGCCTCCCCATCTCCACAGCGTCAGGCGGGGGCCCCGCAGGCTACCCGTCAGGCATCTATCTCTGGT CCCACTCGTCAGGCCAGTCAGGCAGGTCCCGGACCTCGCACTGGGCCTCCCACCACACAGCAGCCCCGGC CCAGCGGCCCAGGTCCTGCTGGACGTCCCGCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGATGTGCC ACCACCCATCACCGCCGCTGCCGGGGGACCCCCGCACCCCCAGCTCAACAAATCCCAGTCTCTGACCAAT GCCTTCAACCTTCCAGAGCCAGCCCCTCCCAGGCCCAGCCTTAGCCAGGACGAGGTGAAAGCTGAGACCA TCCGCAGCCTGAGGAAGTCTTTCGCCAGCCTCTTCTCCGACTGACATCCCACTCTGAGAACCCCTAAACC CCTAGACAGCCCTCTCTGGGTCCTGAGTCCATTTCTCACTTTTGGAACCTCCAAATCCCTTGAGAACCCC TCTCCTGGTTCTCCTAGAGCCCACTTCTCATTCCTCAGTATGTCCCTTGAGAAACCTGGTCCTAAATCCA GTTCTCACATTTGGGAATCCCCAAGTCCATTTAGAATCCTGTTCCTGGTCACCTCCAGATCTGTTTGAGA ACCTCCAAACTCCTGAAGACCTCTACTTCTGGTACATCTAAAGGGTGATTTCTTCCCTGAAAGTTCCTGA ATGGCAGAAAACCTGTTTCTGACCTAATACATCGAATCCTCTCCTGGAGCTCTGAAATTCCTGAAAAACC CTATTCTACTCTTGGTCCTGAATCTCTCCAACACACCTTGCTCCTAATCAGAAATTTCCAATTCCATAAG AAGCCCCTATCCTTAAACCTCTTCCATAGGTCATCTCTCTGGAGATCCACATCTTCAAATGCCACCTACC ATCCACCAAGCAGTCCCTCAAGGACTTCCCTTCACCTCTTCCTCTCCATCCCATTCAATATACTGGAAGT TCCTTCCACTTCTAGGACCCCTAAGTTCCCTCCTCCAGAAACCCCTCCCCAATTTCCTGCCTCTGACAGC TGTCTTTAAATATGCAAACTCCACCCATCCTCAGAATCCTTTGCACAAGGAAGGCCTGTGGTCTCCCACT CCCCTACCTTTGCCAATGTGTTTATCTGTGACTGACTTGGCCTCCTTTTGTGCCATGCTTGGCATATGTG GTCCTCATTCATGCCGCCTGTGGTAATGCGTACAGTGGCACTGTTTTTGTGGTCTGCATCTTCCTCCAAC CTCCACTCCTTGCCTGGACTTGCCCCACCCCTCAGCGGCTCTGAACCCCCAAGAGAAGAGTCGGGAAGCA AAATAAACAAGCAAAGGCCCAGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368098 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368098 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10798 same embryo
	EMAGE:10795 same embryo
	EMAGE:10797 same embryo
	EMAGE:10799 same embryo
	EurExpress:euxassay_003231 same experiment
	MGI:4828553 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source