EMAGE mouse gene expression spatial database

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EMAGE:10798

Slc25a22 solute carrier family 25 (mitochondrial carrier, glutamate), member 22 ( MGI:1915517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003200. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003200_01	euxassay_003200_02	euxassay_003200_03	euxassay_003200_04

euxassay_003200_05	euxassay_003200_06	euxassay_003200_07	euxassay_003200_08	euxassay_003200_09

euxassay_003200_10	euxassay_003200_11	euxassay_003200_12	euxassay_003200_13	euxassay_003200_14

euxassay_003200_15	euxassay_003200_16	euxassay_003200_17	euxassay_003200_18	euxassay_003200_19

euxassay_003200_20	euxassay_003200_21	euxassay_003200_22	euxassay_003200_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10798_wholemount_strong.wlz

10798_wholemount_moderate.wlz

10798_wholemount_weak.wlz

10798_wholemount_possible.wlz

10798_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10798_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 20

spinal cord

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 09 11 12 13 14 19 20

not examined

regional

not examined expression: see section 01 02 03 23

neural retina

weak

regional

weak expression: see section 01 02 03 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5415
Entity Detected:	Slc25a22, solute carrier family 25 (mitochondrial carrier, glutamate), member 22 ( MGI:1915517)
Sequence:	sense strand is shown >T5415 CCACGCGTCCGGTTCCTGGCTGCCTGGGAGGACGCCACGGGGAGGGCATTGAAGTGAATCCAGGTGGCAC AAGACTTGAAATTGCTTTGAAGGTGAGGACATCCCCTCCCCCACCGCCAGCCACCACCACCAGCCACCTA CATCGTCCTCATCAGCCCCTCCCCATCAGTGCCTCCACCAACATCACCCGGCCTGACTCCTGCTTCACTT GGTGTAAATGGCTGATAAGCAGATCAGCCTGCCGGCCAAGCTCATCAATGGTGGCATCGCTGGGCTAATC GGGGTCACCTGCGTGTTCCCCATCGACCTGGCTAAGACACGGCTGCAGAACCAGCAGAATGGCCAGCGCA TGTATGCCAGCATGTCTGATTGCCTCATCAAGACCATCCGCTCCGAGGGCTACTTCGGCATGTATAGGGG AGCAGCAGTAAACCTGACCCTGGTCACCCCGGAGAAGGCCATCAAGTTGGCAGCTAATGACTTCTTCAGA CACCAGCTCTCTAAGGATGGACAGAAGCTGACCTTGCCTAAGGAGATGCTGGCAGGCTGCGGGGCCGGCA CCTGCCAGGTGATTGTGACCACCCCCATGGAGATGCTGAAGATCCAGTTGCAAGATGCAGGCCGCATTGC TGCTCAGAGGAAGATTCTGGCTGCCCAGGCTCAGCTCTCAGCCCAGGGAGGTGCCCAGCCCTCGGTAGAG GCTCCAGCCCCTCCTCGGCCCACGGCCACCCAGCTGACCCGTGACCTGCTGCGGAATCATGGCATTGCTG GCCTCTACAAGGGACTGGGGGCCACGCTACTCAGGGATGTTCCCTTCTCCATTGTCTACTTCCCCCTGTT TGCCAACCTGAATCAGCTGGGCCGCCCATCCTCTGAGGAGAAGTCGCCTTTCTATGTGTCCTTCCTAGCA GGCTGCGTGGCTGGGAGTGCAGCCGCTGTGGCCGTCAACCCTTGTGATGTGGTGAAGACTCGGCTCCAGT CCCTTGAGAGAGGTGTTAATGAGGACACTTACTCTGGGTTTCTGGACTGTGCAAGGAAGATCTGGAGACA TGAAGGTCCCTCAGCCTTCCTGAAAGGCGCATACTGCCGTGCGCTGGTCATTGCCCCGCTGTTTGGCATC GCCCAGGTGGTCTACTTTCTGGGCATTGCCGAGTCCCTGCTGGGGCTGCTGCAAGAACCCCAGGCCTGAG CCCATGGCTGCTTCTCTCCAGCCTATGGGCAGGGGCCAGAACAGGGTGACCAGCACAAGCCTGAGGAGGA GTGGTCTCTCCCCGGTCCTCCTCATTAAGATGGGAAGGCAAGGGGAGGGTGCAGGGTCCACATGGGTGAT GCACACATAAGCCCCTGTGTGGTCCTGAAGGGACAACAAATTGGGATCAAGGTCTTATTTATGTAGAAAA TGCAGAAATCTGTACATTCCTCAAGCCAGTTCTGTCCCATCCTTGTACCTCAACCCAGTCCCACCTGGCC TGAACACCCCATGAGACAGAGCTGGTCTCTGGGCTGGGGCCCCCAGGCCTGGTTTGGGCAGGCAGACCCT ACCCTGTAGTCCACTGGCTCTGGTCTCTGAGGCTCTCCTGGTCTACATAGGGGACCCACACACACCCAGA ATTATTTATTGAATTTTCCGTGCCCATGTGGCTCTCCTGTCCTCCGTCCATCAGTCTTTGCCCCTGCTGC TCTTCCCACCTCATCCAGGACCCCACTCTGCCTCCCCAGCCAGCCTTACAGGAGGCCTGGAGGGTCTTAG CCTCATGTCAAGCTAGGATTCCTAGGCTGGGTATGTTGCCGTAATCAGGTGTCATCCCAGGCACCCCACA CATCCACTTCCCGCTGAGCCTTTGGACCCCCACCATGTGGGACCCAGGTTGCTTGAGGTCTTTGTGTGCC TGCGGTGGGCAGTTGCTCCCCCACCCCCAGCAGTCAGCTGGCCAGGCTGCTTAGGGATGGGGACCCATAA GGGTACAGCTGCCTTTTCCCGTGGAGTCAGCCACCCTGTGCTTGTGTGCTGTTTGTGCCTCAGGACTCTT CTGCTGACCCCGTCCAATAATTTAGTTCTCCAGCTTGGTGACTCTTCTGATAGAGCCCTACTCCTGGACA GAGATTGCTTTTGGGAGGTGGAGTGGGGGCTTGAAGGTCAGGCATGAAGGCCAGTTTAGCGTCTGGGCCT CCCAGCTCTCTGGGCCTCTGTCCATGTTTGGAAAGCCCCACCCCTACAGCCCTGGATCCTCTGTTCAGAA TCAAAGCAGCCCTTCCCTGCCCCGGCCATGTGATTGTGTTTGGTGATTGACTGAAGTGCTGGTGCTGTGT CCCTCAAGCCCAACCCCCTCCATGGAGGCCCCTTCCCAGTGACACTACCTGGGGCTCCAGTCTGGGCTGC CCCTAGTTCACCGTTGCTCCTGGGAGCCACCCCTCCTCTTACGTCTGTACCTTGAAAGCTGCTGCTGCTG CTTACAGAATTATATTTTTTTCTTTTGAAGAGTTTTAAGAAGTTGTAACTTTTTGTGTCTTGTCATGTAA AAAATAAATAAATATTCTAAATAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5367108 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5367108 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10795 same embryo
	EMAGE:10796 same embryo
	EMAGE:10797 same embryo
	EMAGE:10799 same embryo
	EurExpress:euxassay_003200 same experiment
	MGI:4828152 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source