EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10830

Fam82a1 family with sequence similarity 82, member A1 ( MGI:2147043)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001150. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001150_01	euxassay_001150_02	euxassay_001150_03	euxassay_001150_04

euxassay_001150_05	euxassay_001150_06	euxassay_001150_07	euxassay_001150_08	euxassay_001150_09

euxassay_001150_10	euxassay_001150_11	euxassay_001150_12	euxassay_001150_13	euxassay_001150_14

euxassay_001150_15	euxassay_001150_16	euxassay_001150_17	euxassay_001150_18	euxassay_001150_19

euxassay_001150_20	euxassay_001150_21	euxassay_001150_22	euxassay_001150_23	euxassay_001150_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10830_wholemount_strong.wlz

10830_wholemount_moderate.wlz

10830_wholemount_weak.wlz

10830_wholemount_possible.wlz

10830_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10830_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

homogeneous

strong expression: see section 08 09 10 11 17 18 19 20

anal canal

weak

regional

weak expression: see section 15 16

hindgut

moderate

regional

moderate expression: see section 13 18 weak expression: see section 10 11 12 17 19 20

testis

moderate

homogeneous

moderate expression: see section 05 06 07 08 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4858
Entity Detected:	Fam82a1, family with sequence similarity 82, member A1 ( MGI:2147043)
Sequence:	sense strand is shown >T4858 CCACGCGTCCGCCGTTATCTGGGTCAGACACGAAGGCCACCACGCAGGACCTTGGGACCCGCAGGCTGCA CAGGGTTCCACTCGGCCATCCTCTCCTGGCTCGCCCGCTCGCCCGCGGGTCCCGGTTCCCTGGCTCCAGC CCCAGAGAAGCCTGCGCAACGACTCCGGAAGTAAAAGCCCAGAATGCCCCATTCCACAAACAAAGAGCTA ATCCTTGGCATCATGGCGGGGACTGCTGGGATCAGCTTGCTGGCCTTCTGGTACCACAAGGTCCTCAAGC CAAGGACGACCATGAACTTCTCTAAGCTTCTTTCTCTGGGTAAGAAATTTGGCTCACTAACTCTGCCAGA GGAAAGCCACAGTGCGCAAGGAGCATCGGTGGTCTTTCAAAGGAGGCAACTCCAGATCCTGGAAAAGCTA AACGAGCTACTGACCAACATGGAAGAACTGAAGGAGGAAATCAGATTCCTTAAAGAGACAATTCCAAAGC TGGAGGAATGTATACAAGATGAATTCGGAGGGAAGGTGACAGTTCATAAGATCAGTCCTCAGCACAGAGC GAGAAAAAAGAAGGGGACAACCGTTCAACGCTCAGCAACGAGTAACAGCTCAGAGGAGGCAGAAAGTGAA GGGGGGTATATTACAGCTAACACGGACACAGAAGAACAGAACTTCCCGTTCCCCAAGGCTCTTAACACAC ACATAGAGGAGCTAAAGTTGGATGTCCTCCTTCAGAAGGCAGACCATTTGCGGACGAATGAGTCTCACAA AATGGAGAGTTTTGAGCTACTCTGTGACCACAAGGAGAAGTTTAGTGAAGAAACTGAGTTTCTGTGGCGC CTGGCCCGAGCCTATGGAGACATGTATGACCTATCCACAAGTACACAAGAAAAGAAGCATTATGCTAATA TTGGAAAAACTTTAGGAGAGAGAGCTATTACCAGAGCGCCCATGAATGGACACTGCCATCTGTGGTATGC AGTTTTGTGTGGTTATGTATCTGAGTTTGAGGGCTTGCAAAACAAAATTAACTGTGGACATCTATTCAAG CATCTAGAAATAGCCATCCAGCTTTTGCCTGAAGAACCCTTATTATATTACCTCAAGGGAAGATACTGTT ATACTGTCTCAAGACTCAGCTGGATTGAGAAAAAAATGGCTGCTACTCTGTTCGGAGAAATACCATATTC AACGGTTCATGAAGCTTTGCACAACTTCCTTAAGACTGAAGAACTGCAACCTGGTTATTCCATGTCCAAC TATATGTATACAGCCAAGTGTTATGTTGAACTTGGGGAACCTCAGGAGGCTTGTAAATTCTGTAACTTGG CATTGCTACTGCCTGTTGTCACCAAAGAGGACAAGGATGCCCATAAAGAGGTGAAGAAAATGATCAGTTC TTTGAAGAGGTAAATAAAAGACCTTATTCTTCAGCAAACCCAATACCGTCTTCTAGAATTTAACAGATTG TACTTTTATAATGCCTTTCTCCTTAATGTAGAGATTATGTACTCTCATTTGATTTGAAGGTCTAGCCTTG CCTTCTAGACCCACTGCACTATGAGACAAATCATGTTCTTTGGAGGCAGTACTTACTCTGATGTTAGTAT GTAACGATCTCTGGACATGTATACTTCTTTGTTTATTCATGTCAATATACTACAGACTTTAGAATGTTTG TGTAACCAATACGTAATCTAATAAAATGCACCCTAGCAAATGTTATTGTTTCACTAGAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5067179 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067179 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10831 same embryo
	EMAGE:10833 same embryo
	EMAGE:10832 same embryo
	EMAGE:10828 same embryo
	EMAGE:10829 same embryo
	EurExpress:euxassay_001150 same experiment
	MGI:4824763 same experiment
	EMAGE:31371 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source