EMAGE mouse gene expression spatial database

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EMAGE:10833

D14Ertd668e DNA segment, Chr 14, ERATO Doi 668, expressed ( MGI:1277133)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003051. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003051_01	euxassay_003051_02	euxassay_003051_03	euxassay_003051_04

euxassay_003051_05	euxassay_003051_06	euxassay_003051_07	euxassay_003051_08	euxassay_003051_09

euxassay_003051_10	euxassay_003051_11	euxassay_003051_12	euxassay_003051_13	euxassay_003051_14

euxassay_003051_15	euxassay_003051_16	euxassay_003051_17	euxassay_003051_18	euxassay_003051_19

euxassay_003051_20	euxassay_003051_21	euxassay_003051_22	euxassay_003051_23	euxassay_003051_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10833_wholemount_strong.wlz

10833_wholemount_moderate.wlz

10833_wholemount_weak.wlz

10833_wholemount_possible.wlz

10833_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10833_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 05 06 07 21 22 23 24

thymus primordium

moderate

homogeneous

moderate expression: see section 17 weak expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 18 19 20 21 22 weak expression: see section 10 11

vibrissa

moderate

regional

moderate expression: see section 07 08 09 24

lower jaw incisor

weak

regional

weak expression: see section 13 14 16 17 18 19

upper jaw incisor

weak

regional

weak expression: see section 13 14 15 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4859
Entity Detected:	D14Ertd668e, DNA segment, Chr 14, ERATO Doi 668, expressed ( MGI:1277133)
Sequence:	sense strand is shown >T4859 CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCTCAGGCGTGCTTATAGGAGTGC AGGCGCCCAGTCATGGCCCAAGAGACAGCACCACCGTGTGGCCCAGTCTCAAGGGGTGACAGTCCAATCA TAGAAAAGATGGAAAAAAGGACATGTGCCCTGTGCCCTGAAGGCCACGAGTGGAGTCAAATATACTTTTC ACCATCAGGAAATATAGTTGCTCATGAAAACTGTTTGCTGTATTCATCAGGACTGGTGGAGTGTGAGACT CTTGATCTACGTAATACAATTAGAAACTTTGATGTCAAATCTGTAAAGAAAGAGATCTGGAGAGGAAGAA GATTGAAATGCTCATTCTGTAACAAAGGAGGCGCCACCGTGGGGTGTGATTTATGGTTCTGTAAGAAGAG TTACCACTATGTCTGTGCCAAAAAGGACCAAGCAGTTCTTCAAGTTGATGGAAACCATGGAACTTACAAA TTATTTTGCCCAGAACATTCTCCAGAACAAGAAGAGGCCACTGAAAGTGCTGATGACCCAAGCATGAAGA AGAAGAGAGGAAAAAACAAACGCCTCTCATCAGGCCCTCCTGCACAGCCAAAAACGATGAAATGTAGTAA CGCCAAAAGACATATGACAGAAGAGCCTCATGGTCACACAGTAATCAAGAGTAAAGCTTGTGAATGGGAA GAAAGGCAAAGGCAGATGAAGAAGCAGCTTGAGGCACTTGCAGACTTACAACAAACCTTGTGCTCATTTC AAGAAAATGGGGACCTGGACTGCTCAAGTTCTACATCAGGATCCTTGCTACCTCCTGAGGACCACCAGGT CAGATGCCAGGAGTCTCCTGAAGTGCAAGCAGGCTCAGGAGACAGCCTGTGACAGATCCACAGTGCCCTC CCGCTCTGACCTGCTACTGCTCATCCATGCTCCATACCTGACTGGGTGTTTCTTGTCTCCTCTCTTCTCA TCTCACTTGTCCTGCTTTCCCCTGACAGTAAAAGCTGTTCCTCAGGAAAACTGGATGGGGCCTCCATGTT CTCCAAGGATCGAGGAAGTCTTCCTGCCTACCCTGCCCACCCCAGTCAAGGGCAGCAACACCAGAGCTTT GCTCAGCCTTAAATGGAATCTTAGAGCTTTCTCTTGCTTCTGCTGCTCCTGCAGATGGTCTCATCATGGT CTCCTTTCAGTATTAATAACTCCATCAGCATAGAGCAAACTCAACACTGTGCATTGCACACTGTTACCAT GGGTTTATGCTCACTATCATATCACATTGCCAATATTTAGCACACTTAATAAATGCTTGTCAAAACCAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5067268 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067268 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10830 same embryo
	EMAGE:10831 same embryo
	EMAGE:10832 same embryo
	EMAGE:10828 same embryo
	EMAGE:10829 same embryo
	EurExpress:euxassay_003051 same experiment
	MGI:4824195 same experiment
	EMAGE:31371 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source