EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10856

Cbx5 chromobox homolog 5 (Drosophila HP1a) ( MGI:109372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003425. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003425_01	euxassay_003425_02	euxassay_003425_03	euxassay_003425_04

euxassay_003425_05	euxassay_003425_06	euxassay_003425_07	euxassay_003425_08	euxassay_003425_09

euxassay_003425_10	euxassay_003425_11	euxassay_003425_12	euxassay_003425_13	euxassay_003425_14

euxassay_003425_15	euxassay_003425_16	euxassay_003425_17	euxassay_003425_18	euxassay_003425_19

euxassay_003425_20	euxassay_003425_21	euxassay_003425_22	euxassay_003425_23	euxassay_003425_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10856_wholemount_strong.wlz

10856_wholemount_moderate.wlz

10856_wholemount_weak.wlz

10856_wholemount_possible.wlz

10856_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10856_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6229
Entity Detected:	Cbx5, chromobox homolog 5 (Drosophila HP1a) ( MGI:109372)
Sequence:	sense strand is shown >T6229 GCGGCGGTAGCGGTGGCTTGTGGTGCCGCTTTACCACAGAGGAACCGTGCAGGCCTTAGCGTGAGTGATC ACTTTAGATCTTGGGAACCTGGTGGCCATACTCCTTCAGGTGGATCAACAGGCACACGACATGGGAAAGA AGACCAAGAGGACAGCCGACAGCTCTTCTTCAGAGGATGAGGAGGAATATGTGGTGGAAAAGGTGTTGGA CAGGCGCATGGTTAAGGGGCAAGTGGAATATCTGTTGAAGTGGAAAGGCTTTTCTGAGGAGCACAATACT TGGGAACCTGAGAAGAACTTGGATTGTCCTGAACTAATTTCTGAGTTTATGAAAAAGTATAAGAAGATGA AGGAGGGTGAAAACAATAAGCCCAGGGAGAAATCAGAAGGAAACAAGAGGAAATCCAGTTTCTCCAACAG CGCTGATGATATTAAATCTAAAAAAAAGAGAGAGCAAAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA CTGGAACCAGAAAAGATCATCGGAGCAACAGATTCCTGCGGTGACTTAATGTTCTTAATGAAATGGAAAG ACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAACGTGAAGTGTCCACAGATTGTGATAGCATT TTATGAAGAGAGACTGACGTGGCACGCATATCCAGAGGATGCGGAAAACAAAGAAAAAGAAAGCGCGAAG AGCTAAAGGAGGAGGCAGTCTCTGTCGTTTCTCTTTGTATATAATACCTTTACTTTCCTGCCTCCTCTCT CTCCTCCCACCCCTTTCCATCCTAAACGCATCCATAAACCACTGCGCTCATCACTGTGCTCCACGGGGGA GATGCTGGTATTGGTTCTCCTGTAATATAACTGATGATCTGATTCCTAAGTGTAGCTCTGTGAACGTCAG GCATTACGTCATTCACATGCTGTGTGGTTCCCATAGTGTTCTCTTTCACCCTGATCTTTTCCTTCTGTTC CCCTGTAACTTTTTAATCACCTTAGAGTCATGATCTTTGGGGTTGGTTGCTTTGGGAGATTTTATTATGA CGATGACTTCAGTTTCTTGATTGTTATTAGATTATATTGATGACCAGCTGGCCTTTGTTTTGACATACTG GCATAGAAAGGATATTGTCCATCTATTTAGAAAACTATTAAGGGCAACCTACCTGTTGTGTCTTTTCCGA TCTCATTTAGCTCTGCCAGGAGAATACAGGGTCCCTGGTGTGGTCTTGGGCCGCTCCTGACTATCCTCAC ATACCCTCCCATAACAATTCAGTTCTCTTAGGGCTTGAAACTTCACATTTAAATCTGTCAAGGCGCTCTG TTTAGCCCTCACACTTTCTTGGCCTTGTTACTTTCAATCCCTGATTCTGCTTCTGTAAAGTGGTATCAAT TTGTTATTAAATGACAAGATTAACTGTGGAGTCTGTAAGCTGTAGTGCAGGAAGATAATAGGATGGGGCT GGATACAGTTATCACGTGGTGTGTGTGTATTCTTTCCTTTATCCCTCATATTTAGACTGTATTTATGTAG GTGTGAGTGATTGCCTGGTGCTTGCTTGTGCCAAGGTGCTGGGCACCCTCTAACCCTGCCAATTTTTATG GCCTCCCAAAGCATTCCTGTACCAAAGAGGCATCAGCAGATCCATGCCATTCCCTTCCGTGCCATTATTT TAAGTTGGGAATTTTTGGAGAGGAGCCACTAGCCACAGTGTCACTGGCTTTTACGTCCTTGCAGCAGTGA TGCTCTTTAATTTACTGCCTCATGGCTTGTGGATTTGCCACCAAGTCCTCAGAGGATTAATGTTCCTGTC CTCTCCACTTGAGTCTGAGGATTTTTAGTTGTAGCTTATTTTAAAAACATAGAAGATTATGGCCTCAGAA ACCGACAGGTCTTGATATTAACTTTTTGACACAACCTTCTCTCACAACAAACTACTTCCTTTTTGGTTTA CTCTTGCTTTGCTATTATTTCTGCTCCTATTCTACTTTTTGTCCCCTGAAAAATGCCCATCTTCTCTTCA TTTCTTTCTTCTTGTATAGTTGAGGAAGCTGGAGAATCAGGACTAAAAGTAAGGAAATAACAGAGTTTGG AGCTGAGCAGTCTTGTCCCAGCTCCAACGCTGAGGAAAAAGAATAAACGCTGAAGTAGCCTTAATTCCAG ACTTCGTCAGCTACTGAGCTGCTTTTGTTGTTGCGTTTGATTTTTTTAAAGCAAAGCTGTCTGGGCCAGA GCATCTAGCCACCGGGTTGTTGATAGTAATGAGAGGAATGGATGGAGTGCTTCAGTCCAGAGATGAGCCA GATAATATGCAAACCCTAGACCCATTCACAGCATTTGTTAACACTGCTTTCCTGCTCAGCTGCTACTTGA GTTCTGTGTTCTTGAATAAATTATGCCAACAATTATACTGCACCGTTGAGAAGGCAGCTTGTACTGCAGT GACATGGTAGACAGACAGTGAAGCTTTGCCTTACTTAAGTCAGCCAGGAACAACTTGTGAATGGTAATGT GGTGGGAGAATAGCACCTAACCCATACCTCACATGGGTGAGCTTTTTCTCTCTGTAGTCCGTAGTTGTCT GAAGATGTTAGAGGCAATATCAAATGCTGGATAAGTGACCGATGTCTGTACTTAACTTGATGAAATGAAG GATGGTTAGAGAGCAGATCTGTCCTTCAGGCAACCAAGGCTAGTAGTAATTAGGGCAGATGAAAATAGCA CAGAAGTAGCCAGAGCTCCTGAGGATCTTGACTGGCAACTAGAGGAAAGGACCACCCACTGAGTAGTCAG GAGTAGTGGGCATGTACATTGCAGTGACCATATTTTTTAAAGCAAAATCTTAGCCTTTGGGCAACCATAA CAATGATATTTAGTCCTATATAATATAAACCATAATAATGAGATTAGTCCTAAGAAGTCTGGCCTGGTGA CAAAAAAGTACACAGTCCCAGACTGATACAGGGGTACTGTATGTTCAAGGCCTGTTTGGGAACTTAGTGA GACCCTGACTCAAGATAAAAAATAAAAGGACCGAGGATACAGTCTGAGAGTTAATGCCTTGTAATGCAAA AAATGAAGGGAAAAAGTGTCCTGCTGGTTTTAATGCTCTTTGTAATTTTTTTCTCTTAAAAGTTCATGGA GTATTGGTTTTCTGTTAACTATTACCAAAACATCCTTGTTTTCTTCTTAGTACTTTTCCTGGTTATATAT AGAAATAGGGAACCGGGATTCTAGTATTATCTTCAACACTCAGTGTAAAGAAGCCAGACACCCATCTGTG CCTCCAGCATGTGTTGTGCCAAGTAGCTTGTTTCTGCTGGAGAATTGCAGCCTTGGACAGAAGTGTTTCC TAGAGCTATCTTCCCTCTAAAGAGTTCACAGCCAAAGGTGAAATACTCCTAAACACTTGACTATATTCTC CTGTGTTAATCCTCTTTCAAAACTAATTTTGTGGTACTGGGACTTGAGCTTAGACATGCCAGGCAAGTGC TGTGCCACTGAACTGATGTCTTTTTCTGCCCCTTCCCCGCCATACACCATTTTATTGTTGTTCTAAAGAC TTAGTCTCTGATACCAGGCTAGCCTCAACTTGCTGTATAGACAAGTTGAAAAGAATTTTTTTTTTCATCT GAAGACAGCTACAGTGTACTTACATATAAATAATAAATAAATATTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499489 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499489 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10858 same embryo
	EMAGE:10855 same embryo
	EMAGE:10857 same embryo
	EMAGE:10854 same embryo
	EMAGE:10853 same embryo
	EurExpress:euxassay_003425 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source