EMAGE mouse gene expression spatial database

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EMAGE:10857

Cstf3 cleavage stimulation factor, 3' pre-RNA, subunit 3 ( MGI:1351825)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003441. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003441_01	euxassay_003441_02	euxassay_003441_03	euxassay_003441_04

euxassay_003441_05	euxassay_003441_06	euxassay_003441_07	euxassay_003441_08	euxassay_003441_09

euxassay_003441_10	euxassay_003441_11	euxassay_003441_12	euxassay_003441_13	euxassay_003441_14

euxassay_003441_15	euxassay_003441_16	euxassay_003441_17	euxassay_003441_18	euxassay_003441_19

euxassay_003441_20	euxassay_003441_21	euxassay_003441_22	euxassay_003441_23	euxassay_003441_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10857_wholemount_strong.wlz

10857_wholemount_moderate.wlz

10857_wholemount_weak.wlz

10857_wholemount_possible.wlz

10857_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10857_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 17 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 21 22 23

facial vii ganglion

weak

regional

weak expression: see section 05 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 19 20 21 22 23 24

vagus x ganglion

weak

regional

weak expression: see section 09 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 14

dorsal root ganglion

moderate

regional

moderate expression: see section 11 13 17 18 19 weak expression: see section 09 10 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6198
Entity Detected:	Cstf3, cleavage stimulation factor, 3' pre-RNA, subunit 3 ( MGI:1351825)
Sequence:	sense strand is shown >T6198 GCGGCGGCGGCGGCGGCAGCGGATTTGGCGGCGGCTTTGGCTCTGAGGGACAGGGGTAGTGTTCCTGGTT TTGGTGGGGTTTGCCTTTAGTTTAATTTTCCTTTTCTAGCTTCCCTTTGCCGGCTGGTGCGTACGTTGTA GTCCCCCGAGGCCATGTCAGGAGACGCAGCCGCGGAGCAGGCAGCGGAATATGTCCCAGAGAAGGTGAAG AAAGCGGAAAAGAAATTAGAAGAAAATCCATATGACCTTGATGCTTGGAGCATTCTCATTCGAGAGGCAC AGAATCAACCTATAGACAAAGCACGGAAGACTTACGAACGCCTTGTTGCCCAGTTCCCCAGTTCTGGCAG ATTCTGGAAACTGTACATTGAAGCAGAGATTAAAGCTAAAAATTATGACAAGGTTGAAAAGCTATTTCAA AGATGCCTTATGAAGGTTTTGCACATTGATTTATGGAAGTGTTATCTTTCATACGTCAGAGAAACCAAGG GTAAACTGCCCAGCTACAAAGAAAAAATGGCTCAAGCTTATGACTTTGCACTAGATAAAATTGGAATGGA AATTATGTCCTATCAGATTTGGGTGGATTACATCAATTTCTTAAAAGGAGTGGAAGCTGTAGGATCTTAT GCAGAAAATCAGAGAATAACTGCTGTCCGAAGAGTTTATCAACGAGGTTGTGTTAATCCAATGATCAACA TTGAACAACTCTGGAGAGACTACAACAAGTATGAAGAGGGTATCAATATTCATTTAGCTAAAAAGATGAT TGAAGATCGGAGTAGAGACTACATGAATGCTAGACGTGTAGCAAAGGAATACGAGACAGTCATGAAAGGT TTGGACCGCAATGCTCCTTCAGTGCCTCCTCAGAATACTCCTCAAGAAGCTCAGCAAGTGGACATGTGGA AGAAGTACATCCAGTGGGAGAAGAGCAACCCTCTTCGCACAGAGGACCAGACCCTGATAACCAAAAGAGT TATGTTTGCCTATGAACAGTGCCTCCTTGTGCTGGGCCATCACCCTGATATCTGGTATGAAGCTGCCCAG TATCTGGAACAGTCGAGTAAGCTGCTTGCAGAAAAGGGGGACATGAACAATGCCAAGTTATTTAGTGACG AAGCTGCTAATATTTATGAAAGAGCCATAAGCACGTTATTAAAGAAGAACATGCTCCTTTATTTTGCATA TGCAGATTATGAAGAGAGTCGCATGAAGTATGAGAAGGTTCACAGTATATATAACAGACTTCTGGCAATT GAGGACATTGACCCCACCTTAGTATATATTCAGTATATGAAATTTGCAAGGAGAGCAGAAGGTATCAAAT CTGGAAGAATGATATTTAAAAAAGCAAGAGAGGATGCCAGGACCCGCCATCATGTCTATGTAACTGCAGC CCTCATGGAATATTACTGTAGCAAGGACAAATCTGTTGCCTTTAAGATTTTTGAATTGGGACTAAAGAAA TATGGAGACATTCCAGAATATGTTTTGGCTTATATTGACTATCTCTCTCACCTCAATGAGGACAATAATA CTCGAGTTTTGTTTGAAAGAGTTCTAACGTCTGGAAGCCTTCCTCCTGAGAAGTCTGGAGAAATCTGGGC TCGATTTCTAGCTTTTGAAAGTAATATTGGTGATTTAGCTAGTATACTCAAAGTGGAAAAAAGACGGTTT ACAGCATTCAGAGAAGAGTATGAAGGCAAAGAAACAGCTTTACTGGTGGACAGATACAAATTTATGGATT TATACCCTTGTTCTGCAAGTGAACTCAAAGCACTGGGCTATAAGGATGTCTCCCGTGCTAAGCTAGCTGC TATAATTCCAGACCCAGTTGTAGCTCCTTCCATAGTGCCTGTTCTGAAAGATGAAGTGGATAGAAAACCT GAGTACCCTAAACCAGACACTCAGCAGATGATTCCATTTCAGCCACGACATTTAGCACCTCCAGGCTTGC ACCCTGTCCCCGGTGGAGTGTTTCCAGTGCCTCCTGCCGCTGTTGTTTTGATGAAACTGCTCCCACCTCC TATCTGCTTCCAGGGTCCTTTTGTACAAGTGGATGAGCTGATGGAAATTTTCCGAAGATGCAAGATACCA AACACTGTTGAGGAAGCCGTGAGGATCATCACTGGTGGGGCCCCAGAGCTGGCTGTGGAAGGCAACGGCC CAGTGGAAAGTAGTGCTGTACTGACTAAGGCTGTCAAAAGGCCCAACGAGGATTCAGACGAAGATGAAGA AAAGGGGGCTGTAGTCCCCCCTGTTCATGACATTTACAGAGCACGGCAGCAGAAGCGAATACGATGAGTC GGCTTAAACCTACCTGCCTCTGAGAGAAAAACTCTTACCCAGGACTCCTGTTTTGCCTCTTAAGTCATAT GTTTTAAAGAGACAACACTTTGTTACAAGGTTTTGGAAACAAAGTTGTATTGTCATTGGTGCCTCTGTCA TATGGTTCTTGAGAAAAAACCCAAGCCTGTGTGGATTTTGAATTTGGAGCAGACCTATCCTCTAAGCAAG ATTAGGTTTTAAATAACAGTACAAAATGACAACGCCACATCTTGTTCCTTTTCAAGGGTGTCTTCTATGT GTCACTTGAAGATTTGTAAGTTTTCCACAGTTTTATTTTAAAAACTGGATGGCTTATGATTGTAAAACAT TTTCACATTTTCTGAAAAACGTTTGTTCTCAGTTTGCTTACGTAGTCCTGCCTTACTGTTTGTTTTTATT TATGGCAGAATGTATGGCGACTGTTTTGTAGTTATAAATTTTTAAAAGGGTCCTTTGAAAGGAATAAAAG GACCTCAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498424 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498424 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10858 same embryo
	EMAGE:10856 same embryo
	EMAGE:10855 same embryo
	EMAGE:10854 same embryo
	EMAGE:10853 same embryo
	EurExpress:euxassay_003441 same experiment
	MGI:4824096 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source