EMAGE mouse gene expression spatial database

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EMAGE:10863

Ccdc99 coiled-coil domain containing 99 ( MGI:1917635)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003300. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003300_01	euxassay_003300_02	euxassay_003300_03	euxassay_003300_04

euxassay_003300_05	euxassay_003300_06	euxassay_003300_07	euxassay_003300_08	euxassay_003300_09

euxassay_003300_10	euxassay_003300_11	euxassay_003300_12	euxassay_003300_13	euxassay_003300_14

euxassay_003300_15	euxassay_003300_16	euxassay_003300_17	euxassay_003300_18	euxassay_003300_19

euxassay_003300_20	euxassay_003300_21	euxassay_003300_22	euxassay_003300_23	euxassay_003300_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10863_wholemount_strong.wlz

10863_wholemount_moderate.wlz

10863_wholemount_weak.wlz

10863_wholemount_possible.wlz

10863_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10863_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 03 04 05 19 20 21 22

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 16

vibrissa

moderate

regional

moderate expression: see section 09 22 23 24 weak expression: see section 07 08

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 17 18

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

rest of cerebellum marginal layer

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 weak expression: see section 14 18

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 18 weak expression: see section 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 13 17 weak expression: see section 14 18

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 18 weak expression: see section 19 20

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14

liver right lobe

moderate

regional

moderate expression: see section 11 15 16 17 18 19 20 21 weak expression: see section 22 23 24

maturing glomerular tuft

moderate

regional

moderate expression: see section 08 09 10 11 12 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6233
Entity Detected:	Ccdc99, coiled-coil domain containing 99 ( MGI:1917635)
Sequence:	sense strand is shown >T6233 GCTTACTTAGCAGTGCTGCTGCCGAGTGAAGAGGGAGTTATTTCTGTGCTGAGGTGGAGTTAGTGTCACG AATCTGGGAGTCCAGTGCTCTCCGGACAGGCCTCCGGTTGCAGCAGCCAGGAAGCGTGAGGAGAGAGTAG GTTGAGAACAGACATCATGGAGGCAGATATCACAAATCTTCGGAACAAACTCAAAGAGTGTGAAGACGAG CGACTGAAAGCTGCACACTATGGCCTACAGCTCTTGGAGAGGCAGACGGAACTGCAGAGCCAACTGGATA AATGTCATGAAGAAATGATGATCACAGCAGAGAAATACAACCAAGAGAAGCATGCCCTTCAAAGAGAAGT GGAGCTCAAGAGTCGGATGTTAGACAGCCTGAGCTGTGAGTGTGAAGCTCTGAAACAGCAGCAGAAGGCG CAGCTGGAGCAGCTAGAGGTGCAGCTACATAGGAGCCACCGGCAGGAAGTGAGCGACCTGAAGAACAAGT TAGAAAATCTGAAAGTGGAATTGGATGAAGCAAGGCTTGGCGAGAAGCAGCTGAAGCAGAAGCTGGATCT GCAGGGGGAACTTCTCGCTCACAAGTCAGAAGAGCTCCGGCTCCTGTCTGAGCAGAGAGTGCTCAGTAGC ATGTCTTCGGAGCTGCTGGCTCTTCAGACTGAGCTGACGGCAGCGGAAGGTGTGAAGAATGCCCTCAAAG AAGAGGTGAATGAACTGCAGTACAAACAAGAGCAGCTAGAATGTCTCAATACTTCCTTACTGCACCAGGT AGACAGGCTTAAAGAAGAAAAAGAGGAGCGTGAGAGAGAAGCGGTCTCTTACTATAATGCCTTGGAGAAA GCTCGTGTAGAGAACCAGGATCTTCAGGTACAGCTGGGTCACGCACTCCAGCAGGCGGCGGACCCCAATA GCAAAGGGAACTCGCTGTTCGCAGAGGTGGAGGATCGAAGGGTGGCAATGGAACGCCAGCTCAACTTGAT GAAAGACAAATACCAGTCACTGAAGAAGCAAAATGCGTTCACCAGAGATCAGATGAACAAGATGAAGTTA CAAATCTCCACATTGCTGAGGATGAGGGGCTCGCAGACTGAGTTTGAACAGCAGGAGCGGTTGTTTGCCA TGATAGAACAGAAGAATGGTGAAATAAAACACCTTTTAGGGGAAATTAATAAACTGGAGAAATTTAAGAA CTTATATGAAAGTATGGAATCGAGGCCTTCCACATCAGACACCGCTTGTGTGCTAGAAGACAGCACCTAT TACTCAGATCTACTTCAGCTGAAGCTTGATAAGCTAAACAAAGAAAATGAAAGCACAAAGGACGAGCTGT CCATCCAGAGGATGAAGGCTTTATTTGAGAGCCAGCGGGCCCTGGATATTGAGCGGAAACTGTTTACAAA TGAAAGACACCTGCAGCTCTCAGAAAGTGAAAACATGAAACTGAGAGCGAAGCTAGATGAACTCAAGCTG AAGTATGAACCGGAAGAGAGAATTGAAGTACCTGTACTCAAAAGGAGGCGCGAGGTGCTGCCATTGAATA TAACCACGCCAGAAGAAACAGAAGAAACAGCTGCTGCCAGTGCCACTGAGGATGGAGTTTCTAGACTCCC ACCTCACAGGGAGGAGGAATCCTGTCTTAATAGCTTAAAAGATAACACTGTGCAGTGGAAACAGCCAGCC TCTTCATGTGTGCAGCCAGCCAGCCTCTCTCCTCATAAGAACCTGCATCTGGATACACAGCCGAAGAAGG AGAAGAAGTGTGTGAAGCTTGTGGATAGTCCGGCCAACATTGAGGTCTTACATGAACAAAGTGGGAATAC CCCCAATTCTCCCAGGTTAACTGCAGAATCAAAGCTTCCAACAGAAGTGAAGGAAAGGATAGAAACTACA AGCAAATTGGGAAAAGGAGCTTGTAAGAAATCACACAACATCATATATGTGTCCTCAAAGTCAGCCCCAG AAACGCAGTGCTCACAGCAGTAGTGAGGCCTGCGCGCTGGGCCACGCTAACCATCGCTCCTATGCGTGGG ACACCCTTGTGGAGCTTTGCATAGGTCAGTGCTGAAGCCTGGCGTGCTCGCACCGCTGTGACCAAGACTT CCGATGTGGCTGTGATTCTGACCCATAAGTCCTTAACTGATGATCCTTCTCAGAACTGTGGGTCACAGGG AACAGACCAGATCAGAAGAGAATGGGAGAATTTAAACCTACTTCCTGTGTTGGATGTGGAGTTTGAACAT GGTTAAATAATCTGTCATAAGAACCAAAAATTAGAAAATGGAACACTTAAAATTATTTATAATTTTCTTT GTACATCGTTTTGCACTTAGATTTTCCTTGTCTTGTAAAAATAATGAAATTGGCTTGAAAATATATATTA CTACAAAATGTTTTAAAGTGTGAAGTACCTATATTCATATGTGTAATTTAGATTTTATGACTCAAACATC AATAATTTTGAAATTTAAAATAAAATTGCAGTAATTTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499487 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499487 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10861 same embryo
	EMAGE:10862 same embryo
	EMAGE:10860 same embryo
	EMAGE:10859 same embryo
	EurExpress:euxassay_003300 same experiment
	MGI:4823694 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source