EMAGE mouse gene expression spatial database

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EMAGE:10919

Prim2 DNA primase, p58 subunit ( MGI:97758)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003376. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003376_01	euxassay_003376_02	euxassay_003376_03	euxassay_003376_04

euxassay_003376_05	euxassay_003376_06	euxassay_003376_07	euxassay_003376_08	euxassay_003376_09

euxassay_003376_10	euxassay_003376_11	euxassay_003376_12	euxassay_003376_13	euxassay_003376_14

euxassay_003376_15	euxassay_003376_16	euxassay_003376_17	euxassay_003376_18	euxassay_003376_19

euxassay_003376_20	euxassay_003376_21	euxassay_003376_22	euxassay_003376_23	euxassay_003376_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10919_wholemount_strong.wlz

10919_wholemount_moderate.wlz

10919_wholemount_weak.wlz

10919_wholemount_possible.wlz

10919_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10919_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 13 14 15 weak expression: see section 12

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 18 19 20 weak expression: see section 10 17

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 22 23

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 16 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02 14

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

lower jaw incisor

weak

regional

weak expression: see section 12 13 16 17 18

lower jaw molar

weak

regional

weak expression: see section 07 08 09 19 20

upper jaw incisor

weak

regional

weak expression: see section 12 13 16 17 18

upper jaw molar

weak

regional

weak expression: see section 07 08 09 19 20

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 weak expression: see section 10 11

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 weak expression: see section 12 13 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 05 06 22 23 24 weak expression: see section 01 02 03 04 07 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6699
Entity Detected:	Prim2, DNA primase, p58 subunit ( MGI:97758)
Sequence:	sense strand is shown >T6699 CGGCTGTCAGCGCGGGTGTTTCTTTCAGCCTGGCTGCTGGCTGGTTAAACAATCTACTGCGGCTTTATTT GGGACGGAGAGAAGCCGTGGAGTTGGAAGCTTGTGAGCTGACCTGGTGGGACCGAGTGACAGGTGGTCAA GATGCAGTTCTCAGGAAGGATCCGGAAGAAGCTGCGATTGGCGGGTGACCAGAGAAATGCTAGTTACCCT CACAGCCTTCAGTTTTACCTGCAGCCGCCTACTGAAAACATATCGTTGACGGAGTTTGAAAACTTGGCTT TTGACAGAGTTAAATTGCTTAAAGCAATTGAGAACCTCGGTGTGAGCTATGTGAAAGGAACCGAACAATA CCAGAGTAAATTGGAGGCCGAGATTCGAAAGCTCAAGTTTTCCTACAGAGAGAACCTGGAGGATGAGTAT GAGCCACGGCGAAGGGACCACATCTCCCACTTTATCCTACGCCTCGCGTACTGCCAGTCGGAAGATCTTA GACGATGGTTTATTCAACAAGAAATGGATCTGCTTCGGTTTCGATTCAGTATTTTACCAAAGGATAAAGT CCAGAGTTTCTTGAAGGATAGTCATTTGCATTTTGAGGCTATCAGTGACGAGGAGAAGACCCTTCGGGAA CAGGATATCATGGCGTCCTCTCCAAGCCTAAGTGGGATCAAGTTGGAGTCAGAGTCAGTTTATAAGGTCC CCTTTGCTGATGCTCTGGACCTGTTCAGAGGAAGGAAAGTCTACTTGGAAGATGGCTTTGCTTATGTGCC ACTTAAGGACATCGTGGCCATTATCTTGAACGAGTTTAGAGCCACGCTGTCTAAGGCTTTGGCACTAACG GCCAGGTCGCTGCCTGCTGTGCAGTCCGATGAACGACTTCAGCCTCTGCTCAACCACCTCAGTCATTCTT ACACTGGTCAAGATTATAGTACCCAGAAGAACACCGGCAAGATTTCCTTAGATCAGATTGATTCGCTGTC TACAAAATCCTTCCCACCTTGCATGCGTCAGCTGCACAAGGCGCTGAGGGAGAACCACCATCTCCGTCAC GGAGGCCGGATGCAGTACGGCCTGTTCCTCAAGGGCATTGGGCTAACGCTGGAGCAGGCATTGCAGTTCT GGAAGCAGGAGTTTATCAGAGGAAAGATGGACCCAGACAAGTTTGATAAAGGTTACTCTTACAATATCCG GCATAGCTTCGGAAAGGAAGGCAAGAGGACAGACTATACGCCTTTCAGTTGCATGAAGATTATCCTGACC AACCCACCAGGCCAAGGGGATTATCATGGGTGCCCCTTCCGTCACAGTGATGCAGAGCTGCTGAAGCAGA AGATGCAGTCCTACAAGATCCCTGCCTCGGGGATCAGCCAGATTTTGGATTTGGTAAAGGGGAATCATTA CCAAGTAGCCTGTCAGAAGTACTTCGAGATGACACATAATGTGGACGATTGTGGCTTTTCTTTGAATCAT CCAAATCAGTTCTTTTTTGAGAGCCAGCGCATCCTAACTGGTGGCAAAGATATCAAGAAGGAAATCAGTC AGCCAGAAACACCTCAGCACAAACCTAGCACCCAGAAGACCAGGGATGCTGCATCTGCGCTGGCCTCCCT AGACTCCTCCCTGGAAATGGACCTGGAGGGGCTGGAAGAGTACTTTTAGTAAATGATGTGGCCACTGTAG TATCTCCTTTCCCCCAGCGAATACATTCCTGTTTGAAAGGTTTACAAAGGCACACCCATGCACACACACA CATTTACTGGGAAGCAGGCTTGCTTCCACCTTACTTTTGACAGAATGGGAAAGGAAGCATTCCTATTGAA ACTGTTAAAATCCTCAAGCAGCAAGACAATTAAAACTAACTTTTAAAGTTTTGTATAATGTTGGCCAAGT CCTCTTTTGGATTGTTTTTGCTAATAAACAACATGTATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3602929 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602929 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10920 same embryo
	EMAGE:10924 same embryo
	EMAGE:10923 same embryo
	EMAGE:10922 same embryo
	EMAGE:10921 same embryo
	EurExpress:euxassay_003376 same experiment
	MGI:4827413 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source