EMAGE mouse gene expression spatial database

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EMAGE:10921

Homer3 homer homolog 3 (Drosophila) ( MGI:1347359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003484_01	euxassay_003484_02	euxassay_003484_03	euxassay_003484_04

euxassay_003484_05	euxassay_003484_06	euxassay_003484_07	euxassay_003484_08	euxassay_003484_09

euxassay_003484_10	euxassay_003484_11	euxassay_003484_12	euxassay_003484_13	euxassay_003484_14

euxassay_003484_15	euxassay_003484_16	euxassay_003484_17	euxassay_003484_18	euxassay_003484_19

euxassay_003484_20	euxassay_003484_21	euxassay_003484_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10921_wholemount_strong.wlz

10921_wholemount_moderate.wlz

10921_wholemount_weak.wlz

10921_wholemount_possible.wlz

10921_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10921_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6695
Entity Detected:	Homer3, homer homolog 3 (Drosophila) ( MGI:1347359)
Sequence:	sense strand is shown >T6695 GCGGGGCGCGCGGAGGGCGCGGGCCGGAGGCTGCAGCGACGACGACGCGGGGGGACAAACAAGTGACTCC TGTCACCCCTCTGGGTGCCCCTTAGAGGCCATCCAGCCCAACCTGACCAATGTCCACAGCCAGGGAACAG CCAATCTTCAGCACCCGGGCGCACGTATTCCAGATCGACCCCACTACAAAGCGGAACTGGATCCCCGCCG GCAAGCACGCACTTACCGTGTCCTATTTCTATGATGCAACCCGAAATGTGTACCGCATCATCAGCATCGG GGGTGCCAAGGCCATCATCAACAGCACTGTCACTCCCAACATGACCTTCACCAAAACCTCTCAGAAGTTC GGGCAATGGGCAGACAGTCGAGCCAACACTGTCTACGGCCTAGGCTTTGCCTCTGAACAGCAGCTGACCC AGTTTGCTGAGAAGTTTCAGGAGGTGAAAGAAGCTGCCAGGCTGGCTCGAGAGAAATCTCAAGATGGTGG AGAATTCACTAGTACTGGCCTGGCCCTTGCCTCCCATCAGGTTCCTCCAAGCCCCTTGGTCAGCACCAAT GGTCCAGGCGAGGAAAAGCTGTTCCGTAGCCAGAGTGCGGACACCCCTGGCCCCACCGAGCGGGAACGGT TGAAGAAGATGCTGTCAGAAGGCTCTGTAGGGGAGGTCCAGTGGGAGGCAGAGTTCTTCGCGCTTCAGGA CAGCAACCAGAGGTTGGCGGGAGCCCTTCGGGAGGCCAACGCAGCGGCCACTCAGTGGAGGCAACAACTG GAGGTCCAACGTGCAGAGGCTGAACTCTTGCGGCAGCGGGTAGCAGAGCTGGAGGCCCAGGTGGCTGTAG AGCCAGTCCGGGCAGGAGAGAAAGAAGCAACCAGCCAGTCGGTGGAGCAGCTGGAGGCTCGGGTGCAGAC CAAGGACCAGACTTTGAAGAATCAGAGCACTGGCACCCGAGAGGCTCCAGACACTGCCGAGCGCGAAGAG ACACAGCAGCAAGTTCAGGACCTGGAGACCCGGAATGCAGAGCTGGAGCAGCAGCTGCGGGCGATGGAGT GCAACCTGGAGGAGGCGCGGGCCGAGCGGGAGCGCGCACGGGCGGAGGTGGGCCGGGCTGCGCAGCTGCT GGATGTTCGGCTGTTTGAGCTCAGCGAGCTGCGTGAAGGCCTGGCACGCCTGGCAGAGGCAGCACCCTAG TCTGCCATGGAGTGTCTGCGGCCTCAAGGCGCCCTGGCAGGGGCCAGGGGACCCCAGCTGTCTCTGAGCT TTGCACTGTGTAGAGTTTTCTAGAATCCTTGGGCAGTGCTTCTACCCAGGTTACATTTCTACGTGTGGCG TTGCTGTCCCTGGCTGCTGCTGCCCTGCGCCCCAGGGACACTGCGAGGGAAGGCTGCACTAGTCATCCCC ATGGGGCAACAGAGGCTTTGGGATCCTGAGACCTGAAGGCCCTGTACTCATCCCACCCCATTCTCAAGTC AGACTGACAACTTCAAAGAGTGTTTACTGAAGTCAGGGGCCACCAGCACCAGGTTTACAGCTCAGTCCTG AGCCTCAGCCTGGGCTGGCTCTTGGGGCCGAGATCTGGGAGGACGCGACCGTCGGACAGTGCTCCCTGCT TTCTGCCGCCGAAGTGTCTGCCCCACTTTCTCCTTGAAGCGTCGGTTTTGTTGCTTGATCTTGGCCAGCT CAGCTTTGCGTTTGGCCTCCAGGTCTGGGTCCTGCGGAAGGGAGCTGAGAATGTAACTGGGCAGCTTCCC AGGGACTGGCTCCCCCACCCCTACCCGTCCCCAGGTCCCACCCACCCTTACTGGCCACACTCTTATGCCT GTCCCTGCATACCCATGCCTCCCTATACTACCTTCCCCTCCAGGATCATCTGTTTCCGCTTGTTGATCTC TTTCTTTTCATCAAAATGCGAAGCCTCCAGTTTCTAGGGGTGGGGAGGGGAACAGGTCAGTCAGGCCTGG GGCAGGAAGCCCCGCCCACCTCACCCCACTCCACCCTACCCTGACAGGCTGGCCACACTTACTATTTCGC ACTCCCTTCGCACTACGTTGACCTGCGTGAGGATTTGTAGAACCTCAGCCTCCTCCACCACCAGCTCTGC CAGCTGCTGCTCTGCAGGGACAGGAAACACTGAGTTGGGCTGGGAGTGCAACCAGCCCTCTGCACCCCCA GCTCTGGATGTCTGGATCCAACCAAATGTGGACTGATGATATTTAGAAAAAGCAAAATGCTGCCAAGCTT GGCAGCACATGCTTGTCATCACAGCACTGGGAGGTGGAGGCAGGGGGATCACTCGTTTCAGCTGAGTTCC AGGCCAGCTCTGTAGAGCAAGAATCTGTCTCAAATTAATGACTGAATAAACAAATGAACAAGTAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602414 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602414 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10920 same embryo
	EMAGE:10924 same embryo
	EMAGE:10919 same embryo
	EMAGE:10923 same embryo
	EMAGE:10922 same embryo
	EurExpress:euxassay_003484 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source