EMAGE mouse gene expression spatial database

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EMAGE:10927

Emilin1 elastin microfibril interfacer 1 ( MGI:1926189)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003366. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003366_01	euxassay_003366_02	euxassay_003366_03	euxassay_003366_04

euxassay_003366_05	euxassay_003366_06	euxassay_003366_07	euxassay_003366_08	euxassay_003366_09

euxassay_003366_10	euxassay_003366_11	euxassay_003366_12	euxassay_003366_13	euxassay_003366_14

euxassay_003366_15	euxassay_003366_16	euxassay_003366_17	euxassay_003366_18	euxassay_003366_19

euxassay_003366_20	euxassay_003366_21	euxassay_003366_22	euxassay_003366_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10927_wholemount_strong.wlz

10927_wholemount_moderate.wlz

10927_wholemount_weak.wlz

10927_wholemount_possible.wlz

10927_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10927_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body-wall mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 moderate expression: see section 16 17 18 19 20 21 22 23

medullary renal tubule

moderate

regional

moderate expression: see section 08 09 10 11 12 18 19 20 21 22 not examined expression: see section 13

cranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 weak expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5966
Entity Detected:	Emilin1, elastin microfibril interfacer 1 ( MGI:1926189)
Sequence:	sense strand is shown >T5966 CTGAAGCAGCGGCATCCCCTGGAAGTGCGGTATAGGCATTATTGGTCAGACCAAGATGTGCCTCTAAGGG CAGCAAGGAACATTTCACCATGGCCCCCAGAGCCCTCTGGAGCTGCTATCTCTGCTGTCTGCTGACCATA GCCACGGAAGCTGCCAGCTACCCTCCTCGAGGTTACAGCCTCTACACAGGAGGCACTGGGGCCCTGAGTC CTGGGGGACCCCAGGCCCAGAACTCACCACGGCCTGCCAGCCGCCACAGGAACTGGTGTGCCTACGTGGT GACTCGGACAGTGAGCTGCGTCCTTGAGGATGGAGTGGAGACCATTGTCAAGCCAGACTATCAACCCTGT GGCTGGGGCCAGCCCCACTGTTCCCGAAGTATCATGTACCGGAGCTTTCTTCGCCCTCGCTATCGGGTGG CTTACAAGACAGTGACAGACATGGAGTGGAGGTGCTGTCAGGGTTATGGGGGCGATGACTGTGGAGAGGG CCCTGCTTCCGTTCTGGGCCCAGCACCCTCCACACCACTTCCCCGGCCCAGGCCTGTGCGCCCCAACCTG TCTGGCTCCAGTGCCGGCAGCCATCTCAGTGGACTGGGAGGAGAAGGTCCTGTTGAGTCAGAGAAGGTGC AGCAGCTAGAGCGGCAGGTGAAGAGTCTGACCAAAGAGCTGCAAGGCCTTCGGGGTGTCCTGCAGGGGAT GAATGGGCGCCTAGCAGAAGATGTACAGCGAGCTGTGGACACAGTCTTTAATGGAAGGCAGCAGCCAGCA GATGCAGCTGCCCGCCCGGGCGTGCATGAAACCCTCAGTGAGATCCAGCAGCAGCTGCAGCTCCTGGACA ACCGTGTCTCCACTCATGACCAGGAGCTGGGCCACCTTAACAACCATCATAATGGAGGCCCTGGTGGAGG TGGCAGGGCCTCAGGCCCTGTCCCAGTTCCTTCTGGCCCCAGTGAAGAACTGTTAAGGCAGCTGGAACGG CAGCTGCAAGAGTCTTGCTCAGTGTGCCTGACGGGGCTGGATGGCTTCCGCCAGCAGCAGCAAGAGGATA GGGAGCGGCTGCGAACCCTGGAGAAGCTAATGTCCTCTATGGAGGAGCGGCAGCAACAGCTTGTGGGACC TGCCATGGCCAGGAGACCCCCTCAGGAATGCTGCCCCCCAGAGCTGGGTCGTCGAGTGTCTGAGCTGGAG CGAAGGCTAGATGTAGTGACTGGCTCACTGACAGTGCTAAGTGGACGCAGAGGTTCTGAGCTTGGAGGAG CAGCTGGGCAGGGGGGCCACCCTCCAGGCTACACCAGCTTGGCCTCCCGCCTTTCTCGCCTGGAGGACCG CTTCAACTCTACCCTAGGTCCCTCAGAGGAGCAAGAGAAGAACTGGCCTGGAGGACCAGGGAGGCTGGGC CACTGGTTGCCTGCTGCTCCAGGACGGCTAGAAAAGCTGGAGGGACTACTAGCCAATGTGAGCAGGGAGC TGGGTGGCCGCATGGATCTGCTGGAAGAGCAGGTGGCAGGGGCTGTTCGGACTTGTGGGCAGATTTGCTC TGGGGCACCCGGGGAACAGGATTCTCGGGTCAATGAGATCCTCAGTGCCTTGGAACGCAGGGTGCTGGAC AGCGAGGGCCGGTTACAGCTAGTGGGCTCTGGCTTGCATGAAGCAGAGGCAGCAGGGGAGGCTCAGCAGG CCGTGTTGGAGGGACTGCAAGGGCTCCTGAGCCGGCTTCGGGAGCGCATGGATGCACAGGAGGAGACTGC AGCAGAAATCTTACTGCGCCTCAATCTTACCGCAGCCCAGCTAAGCCAGCTGGAGGGTCTGCTGCAAGCC CGTGGGGATGAGGGTTGTGGCGCCTGTGGTGGTGTCCAGGAGGAGCTGGGCCGCCTTCGGGATGGTGTGG AACGTTGCTCCTGCCCATTGTTACCTCCACGGGGCCCTGGAGCTGGCCCAGGGGTTGGGGGACCAAGCCG TGGGCCTCTGGATGGTTTCAGTGTGTTTGGGGGCAGTTCAGGCTCAGCCCTCCAGGCCCTTCAAGGAGAA CTCTCTGAGGTTATTCTCACCTTCAGCTCCCTGAACGACTCACTCCACGAGCTCCAGACCACTGTGGAGG GTCAGGGTGCCGATCTGGCTGACCTGGGGGCCACCAAGGACAGCATCATCTCTGAAATCAACAGACTACA GCAGGAGGCCACGGAGCACGTCACAGAGAGCGAGGAGCGCTTCCGAGGCCTGGAGGAGGGCCAGGCACAG GCTGGCCAATGCCCTAGCTTAGAGGGGCGATTAGGCCGCCTTGAGGGAGTCTGCGAACGACTGGACACCG TGGCAGGGGGACTACAAGGCCTACGTGAAGGCCTCTCCAGACACGTGGCTGGGCTCTGGGCTGCAGTACG GGAAAGCAACAGCACCAGCCTGACACAGGCCGCCCTGCTGGAGAAGCTGCTGGGGGGCCAGGCAGGCCTG GGCAGGCGGCTTGGTGCCCTCAACAATTCCCTGCTGCTCCTGGAAGACCGTCTGCAGCAACTTAGCCTAA AGGACTTCACTGGACCTTCAGGCAAGGCTGGGCCCCCAGGGCCTCCCGGGCTACAAGGGCCTTCAGGCCC TGCAGGACCTCCAGGACCTCCTGGCAAAGACGGACAACAGGGGGCCATTGGCCCACCAGGTCCTCAAGGG GAGCAGGGAGCGGAGGGAGCACCAGCAGCCCCTGTGCCTAGGGTAGCATTTTCAGCTGCCCTGAGTTTGC CACGGTCAGAACCTGGCACAGTCCCCTTCGACAGAGTCTTGCTCAATGATGGAGGCTACTATGACCCAGA GACAGGTGTATTCACTGCACCACTGGCTGGACGCTATTTGCTGAGCGCTGTACTTACTGGGCACCGGCAT GAGAAAGTGGAAGCAGTATTGTCACGCTCCAACCTGGGCGTGGCCCGCATAGACTCGGGAGGCTATGAAC CCGAGGGACTGGAGAATAAGCCTGTGGCCGAAAGTCAGCCCAGCCCAGGCGCTCTGGGCGTCTTCAGCCT CATTCTGCCACTGCAGGTCGGAGACACTGTCTGCATCGACCTGGTCATGGGGCAGCTGGCACACTCCGAG GAGCCGCTCACCATCTTCAGCGGAGCCCTGCTCTACGAGGACACAGAGCTTGAACAGGTGTAGACAGGGT GGACCCGACCACCAAACTTATCTACAGGGGCCGCAGAGCCACCTGGATGGTGGCTACCCACCAAGGCAAG GGCAAGTCTGGCGACCACGTCCCGCTGCGGTTCAGGCATCCAAATCGGTCACTCCGTACGTTGTGACTGC GTGGTCTATGGGACCGAGGCATCTCCTCTTGACCTTTGGCCTCATTGCCGTCCCCACCAGAGCCCTCTCC GGGACCCAAGCTGCGGAAGAGGAACCTACCCTCCAGGGCTCTCCCCAGTCCACACCACCGTACTAAACTA TTCAGGAATAAAGACACTTTTTTTTTTCTACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485057 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485057 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10930 same embryo
	EMAGE:10929 same embryo
	EMAGE:10926 same embryo
	EMAGE:10925 same embryo
	EMAGE:10928 same embryo
	EurExpress:euxassay_003366 same experiment
	MGI:4824542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source