EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10928

Pafah1b2 platelet-activating factor acetylhydrolase, isoform 1b, subunit 2 ( MGI:108415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003490. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003490_01	euxassay_003490_02	euxassay_003490_03	euxassay_003490_04

euxassay_003490_05	euxassay_003490_06	euxassay_003490_07	euxassay_003490_08	euxassay_003490_09

euxassay_003490_10	euxassay_003490_11	euxassay_003490_12	euxassay_003490_13	euxassay_003490_14

euxassay_003490_15	euxassay_003490_16	euxassay_003490_17	euxassay_003490_18	euxassay_003490_19

euxassay_003490_20	euxassay_003490_21	euxassay_003490_22	euxassay_003490_23	euxassay_003490_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10928_wholemount_strong.wlz

10928_wholemount_moderate.wlz

10928_wholemount_weak.wlz

10928_wholemount_possible.wlz

10928_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10928_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5985
Entity Detected:	Pafah1b2, platelet-activating factor acetylhydrolase, isoform 1b, subunit 2 ( MGI:108415)
Sequence:	sense strand is shown >T5985 GGGGCCCGAGGAGGGGACGCGCGCCGGAACGCGGCCGTCGGGATCAAGCGAGCGACTGAAGCGCAACCCG CCGACGCCGCAGCCGCTGAGGGCCCCGCACGGACCCTCTGCCTGTGTGTAGAATGAGCCAAGGAGACTCG AACCCAGCAGCTATTCCACATGCCGCAGAAGATATTCAAGGAGATGACAGGTGGATGTCTCAGCATAATA GGTTTGTTCTGGACTGTAAAGACAAAGAACCAGATGTGCTGTTTGTGGGGGACTCCATGGTACAGCTAAT GCAGCAGTACGAGATATGGCGGGAGCTGTTTTCCCCACTTCATGCTCTTAATTTTGGAATTGGGGGAGAT ACAACACGACATGTTTTATGGCGACTAAAGAATGGAGAGCTGGAGAACATTAAGCCTAAGGTCATTGTTG TCTGGGTAGGGACAAACAACCACGAAAATACAGCAGAAGAAGTAGCAGGTGGGATTGAGGCTATTGTACA ACTTATAAACACGCGGCAGCCACAGGCCAAGATCATTGTGCTGGGTCTGTTACCTCGAGGTGAGAAGCCC AACCCTTTAAGACAAAAGAATGCCAAGGTGAACCAGCTTCTCAAGGTTTCCCTGCCGAAGCTTGCCAATG TGCAGCTCCTTGATATAGACGGGGGCTTCGTGCACTCGGACGGTGCCATCTCCTGCCACGACATGTTTGA TTTTCTTCATCTCACAGGAGGGGGCTATGCAAAGATCTGCAAACCCCTCCATGAACTGATCATGCAGTTG CTGGAGGAAACACCGGAGGAGAAGCAGACCACCATTGCTTGACTGGCCCATCAGTGTTACAGCATCCCGC TTCCTCAGATCAGTTAGACCACTGGCACTACAGAATCCTTTCTTAAGGCACTTTGCATTGTACAATGTTC CTGGATGCTTGTATGTTTGAAGGGGAGGAGGGATTTAAATTGGTCCTGTATATAAAGGTTTTGTTTGAGA GGAGAAAAATTAGCCAAGGAAGATTCATTTGAAACCAGAAGGGGACTTTTTAGTTGTTTATGACACATTT GTAGAATTATCACTTTTTCCTAGGACAACATCAAGCAAGAGTACAATATGAAGATTCTTTTCTTGGCCCT TTTTTCTATGGATTATGTCATGTGTGATAATTATCACATCTACTTGGCTTTAAACTTATTTTTGGTCCAG TTTTAAGGTTCATGAGTTAGTATGTTGTGTTGTTTTGTTTGATTCATGTATCCTCAGTATTTTTATTAAC ACATAGCATCAGATTAAACATGCTTGTCACTCCAATATGGGAGATGCTATAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3486003 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486003 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10927 same embryo
	EMAGE:10930 same embryo
	EMAGE:10929 same embryo
	EMAGE:10926 same embryo
	EMAGE:10925 same embryo
	EurExpress:euxassay_003490 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source