EMAGE mouse gene expression spatial database

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EMAGE:10933

Bzw2 basic leucine zipper and W2 domains 2 ( MGI:1914162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003362. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003362_01	euxassay_003362_02	euxassay_003362_03	euxassay_003362_04

euxassay_003362_05	euxassay_003362_06	euxassay_003362_07	euxassay_003362_08	euxassay_003362_09

euxassay_003362_10	euxassay_003362_11	euxassay_003362_12	euxassay_003362_13	euxassay_003362_14

euxassay_003362_15	euxassay_003362_16	euxassay_003362_17	euxassay_003362_18	euxassay_003362_19

euxassay_003362_20	euxassay_003362_21	euxassay_003362_22	euxassay_003362_23	euxassay_003362_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10933_wholemount_strong.wlz

10933_wholemount_moderate.wlz

10933_wholemount_weak.wlz

10933_wholemount_possible.wlz

10933_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10933_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5925
Entity Detected:	Bzw2, basic leucine zipper and W2 domains 2 ( MGI:1914162)
Sequence:	sense strand is shown >T5925 CGGACGCGTGGGCTCAGCCACTGCCGCCGCCACTGCTGCTGCAGGATCCGCCGCAGCCACCAGTCTCGCG CGTCGTCGCTCCCTCCTTGGACAGTAATTTTATGAATAAGCATCAGAAGCCAGTACTAACAGGCCAGCGG TTCAAAACCCGGAAAAGGGATGAAAAAGAGAAATTCGAACCCACAGTTTTCAGGGATACACTTGTCCAGG GGCTTAATGAAGCTGGTGATGACCTTGAAGCTGTAGCCAAATTTTTGGATTCTACTGGCTCACGATTAGA TTACCGTCGCTATGCAGACACACTCTTTGATATCCTGGTGGCTGGCAGTATGCTTGCCCCTGGAGGAACA CGCATAGACGATGGTGACAAGACCAAGATGACCAACCACTGTGTGTTTTCAGCAAATGAAGATCATGAAA CCATCCGAAACTATGCTCAGGTCTTCAATAAACTCATCAGGAGATACAAGTATTTGGAAAAGGCATTTGA AGATGAAATGAAAAAGCTTCTCCTCTTCCTTAAAGCATTTTCTGAAGCAGAGCAGACAAAGTTGGCAATG CTGTCTGGGATCCTGCTGGGCAATGGGACCCTCCCAGCCACCATCCTCACCAGTCTCTTCACAGACAGCT TAGTCAAAGAAGGCATCGCAGCCTCATTCGCTGTCAAGCTTTTCAAAGCCTGGATGGCAGAGAAAGATGC CAATTCTGTTACCTCTTCACTGAGAAAAGCCAACTTAGACAAGAGGCTGCTTGAACTCTTCCCAGTGAAC AGACAGAGTGTGGATCATTTCGCTAAGTACTTCACTGACGCGGGGCTGAAGGAGCTGTCGGACTTTCTCC GAGTCCAGCAGTCGCTGGGCACCAGGAAAGAACTGCAGAAGGAGCTGCAAGAGCGACTGTCTCAGGAATG CCCCATCAAGGAGGTGGTGCTTTACGTCAAAGAGGAAATGAAAAGGAACGACCTTCCGGAAACAGCTGTG ATCGGGCTGCTGTGGACCTGCATCATGAATGCGGTGGAATGGAACAAGAAGGAGGAGCTGGTTGCGGAGC AGGCACTGAAGCACCTAAAGCAATACGCTCCGCTGCTGGCCGTGTTCAGTTCCCAAGGCCAGTCCGAGCT CGTCCTCCTGCAGAAGGTTCAGGAATACTGCTACGACAACATCCACTTCATGAAGGCCTTCCAGAAGATC GTGGTTCTCTTTTATAAAGCCGACGTTCTGAGCGAAGAGGCGATACTGAAGTGGTATAAAGAAGCACACG CCGCCAAAGGCAAGAGCGTCTTTCTTGACCAGATGAAGAAATTTGTGGAGTGGTTACAAAATGCAGAAGA AGAATCTGAATCAGAAGGCGAAGAAAGCTAAATGTCTCCAGCACACTGCCTAAAAAAGAAAAAAAAAATT AAAACGCCACACTTTTCGGTTGGGAATGCTGAAGCGTTTGGGGAGGGAAACTTGGCTTCTGTTTGCACCA AGGAAATAAATGATAGGCGAGGCCTCCCCCGCTCCCCGGGGAAGGCATTTTTTTCATTTCTGTTTTGTTT CAATGGAGCCCTGAGGCGTCGGCTACTACACTTGGGACTCTACCTCTCACTGTACGCTAACTAAAGCCAT TCAACAAGGGGGTCAGGTAGGAACCTGAAGCTCAAAACTCAGTACACCCCTTTTTTTAGCTGTATTTTCA GGTACTGTGTGAACGCCCCACTGGTGTCTATGTTACAGGGCCACTTCAATAGTTGTGTTTCTTCTGCTCG TGTATGTGATTTGACAAACCAGTTTTTTTAAAATAAACGGCTTTTTAAAAATCAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3482251 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10934 same embryo
	EMAGE:10938 same embryo
	EMAGE:10936 same embryo
	EMAGE:10935 same embryo
	EMAGE:10937 same embryo
	EurExpress:euxassay_003362 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source