EMAGE mouse gene expression spatial database

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EMAGE:10969

Nolc1 nucleolar and coiled-body phosphoprotein 1 ( MGI:1918019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003313. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003313_01	euxassay_003313_02	euxassay_003313_03	euxassay_003313_04

euxassay_003313_05	euxassay_003313_06	euxassay_003313_07	euxassay_003313_08	euxassay_003313_09

euxassay_003313_10	euxassay_003313_11	euxassay_003313_12	euxassay_003313_13	euxassay_003313_14

euxassay_003313_15	euxassay_003313_16	euxassay_003313_17	euxassay_003313_18	euxassay_003313_19

euxassay_003313_20	euxassay_003313_21	euxassay_003313_22	euxassay_003313_23	euxassay_003313_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10969_wholemount_strong.wlz

10969_wholemount_moderate.wlz

10969_wholemount_weak.wlz

10969_wholemount_possible.wlz

10969_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10969_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6199
Entity Detected:	Nolc1, nucleolar and coiled-body phosphoprotein 1 ( MGI:1918019)
Sequence:	sense strand is shown >T6199 CCCACGCGTCCGCGCGTTTTACCCGGAGCATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCT TTATCCCCTTGTGCTCAGATTTCTGCGGGATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCG ACCGGCGCTACACAGCAGGACGCCAATGCCTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAAGTCCA CCAAAGCCCCAAAGGTGAAGTTACAGTCAAATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATC CAGTGACAGCAGTGAGGACAGCAGTGAGGACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCT GCCGCACAGGTCAAGCGAGCCAGTGTGCCTCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCA GCAGTAGTGAAGAATCCAGTGAGGAAGAGGAAGAGGACAAAAAGAAAAAGCCTGTCCAGAAGGCAGCTAA GCCCCAAGCCAAGGCAGTCAGACCTCCTGCGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGAT TCGGACTCCAGCTCAGAGGAAGAAACACCACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTC AGACTAAAGCCGAAGCCAAACCAGGTACACCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGC CGCCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGATGACTCAGAGGAAGAGAAGAAGGCAGCTGCA CCTCCCAAGAAGACTGTACCAAAAAAGCAAGTCGTGGCCAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCA CCCAGAAGAGCTCCAGCAGTGAGGATTCTTCCAGTGAAGAGGAGGAGGGACAGAGACAACCCATGAAGAA AAAAGCAGGTCCCTACAGTTCAGTTCCACCACCCTCTGTTCCTTTACCAAAGAAGTCCCCGGGAACCCAG GCTCCAAAGAAAGCTGCTGCGCAGACACAGCCTGCAGACAGCAGTGACGACAGCAGTGACGATTCTGATT CAAGTTCTGAGGAAGAGAAAAAACCTCCAGCTAAGACGGTCGTCTCCAAGACACCCGCCAAAGCAGCTCC AGTGAAGAAGAAAGCAGAAAGCTCTTCAGACAGCTCGGGTAACGCTGCCCAGAGCTCTGGGCTGCTGGGG TACTCACTGCCTTGGGCTGCTCTTACAGGGCTCCCCTGAGGTTGATTTTGGGGAGCTGTTCATTCTGTCT CCTCTCTCTAGATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCC AAGCCAGCTGTCACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTA ACCAGAAACCTCAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGA GGAGGCCTCCAAGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAG GCCCCTCAGGCTGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTC CTAAGCCCCCAGCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGA GGCCGAGAGCAGCAGCAGCAGCAGCAGCAGCTCTGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCC AAAGCTACTACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAG CCAAGGAAAGTGAGGAGGAAGAGGAGGAGGAAGAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498349 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498349 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10974 same embryo
	EMAGE:10973 same embryo
	EMAGE:10971 same embryo
	EMAGE:10970 same embryo
	EMAGE:10972 same embryo
	EurExpress:euxassay_003313 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source