EMAGE mouse gene expression spatial database

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EMAGE:10974

Copa coatomer protein complex subunit alpha ( MGI:1334462)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003303. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003303_01	euxassay_003303_02	euxassay_003303_03	euxassay_003303_04

euxassay_003303_05	euxassay_003303_06	euxassay_003303_07	euxassay_003303_08	euxassay_003303_09

euxassay_003303_10	euxassay_003303_11	euxassay_003303_12	euxassay_003303_13	euxassay_003303_14

euxassay_003303_15	euxassay_003303_16	euxassay_003303_17	euxassay_003303_18	euxassay_003303_19

euxassay_003303_20	euxassay_003303_21	euxassay_003303_22	euxassay_003303_23	euxassay_003303_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10974_wholemount_strong.wlz

10974_wholemount_moderate.wlz

10974_wholemount_weak.wlz

10974_wholemount_possible.wlz

10974_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10974_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 21 22 23 24

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 07 08 09 10 11 16 17 18 19 20

clavicle

moderate

regional

moderate expression: see section 06 07 08 09 10 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6201
Entity Detected:	Copa, coatomer protein complex subunit alpha ( MGI:1334462)
Sequence:	sense strand is shown >T6201 GTGGAAGCCTTGGGAGTGCCACCGGGAGCGGCGGGACCCGCTAACCTGACCTGGGAGCCCGAGGCCTGAA GGATGCTAACCAAATTCGAGACGAAGAGCGCGCGGGTCAAAGGCCTTAGTTTTCATCCTAAAAGACCCTG GATCCTGACCAGTTTACACAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACCCTAATTGACAAA TTTGATGAACATGATGGTCCAGTGCGTGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAG GCGATGATTATAAGATTAAGGTTTGGAATTACAAACTTCGGCGCTGCCTGTTCACATTGCTCGGGCACTT GGATTATATCCGTACAACGTTTTTCCATCATGAATATCCTTGGATTCTGAGCGCCTCAGATGATCAGACC ATTCGGGTGTGGAACTGGCAGTCTAGAACCTGTGTCTGTGTGTTAACGGGGCACAACCATTATGTAATGT GTGCTCAGTTCCACCCCTCTGAAGACCTGGTGGTATCAGCTAGCCTGGACCAGACTGTCCGCGTTTGGGA TATTTCTGGTCTAAGGAAGAAAAACTTGTCTCCTGGTGCGGTGGAGTCGGATGTGAGAGGAATAACTGGC GTTGATCTGTTTGGAACCACAGATGCCGTGGTGAAGCATGTGCTAGAGGGTCATGACCGTGGAGTCAACT GGGCTGCCTTCCACCCGACTATGCCCCTTATTGTATCTGGGGCAGATGACCGGCAAGTGAAGATCTGGCG TATGAATGAATCAAAGGCATGGGAGGTTGACACCTGCCGGGGCCATTACAACAATGTGTCTTGTGCTGTT TTTCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAAGACAAGAGTATTCGAGTCTGGGATATGTCTA AGCGGACGGGGGTTCAGACTTTCCGTAGGGATCATGATCGCTTCTGGGTCCTGGCTGCCCATCCCAACCT TAACCTCTTTGCAGCAGGCCATGATGGTGGGATGATTGTATTTAAGCTGGAACGTGAGCGTCCAGCCTAT GCTGTTCATGGTAACATGCTGCATTATGTAAAGGACCGATTCCTCCGTCAGCTGGATTTCAACAGCTCCA AAGATGTAGCTGTGATGCAGTTGCGGAGCGGTTCTAAGTTTCCAGTATTCAACATGTCATACAATCCAGC AGAAAACGCAGTGCTGCTCTGCACCAGAGCAAGCAATCTAGAGAACAGTACCTACGACCTATACACCATC CCCAAAGACGCAGACTCCCAGAACCCTGACGCCCCTGAAGGGAAACGATCCTCAGGCCTGACAGCGGTTT GGGTTGCTCGGAATCGGTTTGCTGTCCTAGATCGCATGCATTCACTTCTGATTAAAAACCTGAAAAATGA GATCACCAAGAAAATCCAGGTGCCAAACTGTGATGAAATCTTCTATGCTGGTACAGGCAACCTCCTGCTT CGGGATGCAGATTCCATCACACTCTTTGACGTCCAGCAGAAGCGAACTCTGGCATCAGTAAAGATTTCCA AGGTGAAATATGTTATCTGGTCGGCAGACATGTCTCATGTAGCGTTACTGGCCAAGCATGCCATTGTGAT CTGTAACCGCAAGCTGGATGCTCTGTGTAACATTCATGAGAACATTCGTGTCAAGAGTGGGGCCTGGGAT GAAAGTGGGGTGTTTATCTATACCACAAGCAACCACATCAAATATGCTGTCACCACTGGGGACCATGGGA TTATCCGAACTCTGGATTTACCCATCTATGTCACCAGAGTGAAGGGCAATAATGTATACTGCCTAGACAG AGAGTGTCGTCCTCGGGTGCTAACCATTGATCCCACTGAGTTCAAGTTCAAACTGGCCCTGATCAACAGA AAGTATGATGAGGTACTCCACATGGTGAGGAACGCTAAGCTGGTAGGCCAGTCAATCATCGCTTATCTCC AGAAGAAGGGCTATCCTGAAGTGGCGCTGCATTTTGTAAAGGATGAGAAAACTCGTTTCAGCCTGGCCCT GGAGTGTGGAAACATTGAGATTGCTCTAGAGGCAGCCAAAGCCCTGGACGACAAGAACTGCTGGGAGAAG CTAGGAGAAGTGGCCCTGCTACAGGGCAATCACCAAATTGTAGAGATGTGCTATCAGCGCACCAAAAACT TTGACAAACTTTCCTTCCTGTACCTTATTACTGGCAACCTGGAGAAACTTCGAAAGATGATGAAGATCGC TGAGATCCGAAAGGACATGAGTGGCCATTATCAGAATGCCCTATACCTAGGTGATGTGTCAGAGAGAGTA CGGATCCTGAAGAACTGTGGGCAGAAATCCCTAGCCTATCTCACAGCTGCTACCCATGGCTTAGATGAAG AAGCCGAGAGCCTGAAGGAAACATTTGACCCTGAGAAGGAGACAATCCCAGACATTGATCCTAATGCCAA GCTGCTCCAGCCACCTGCACCTATCATGCCATTGGATACAAACTGGCCGTTACTGACCGTGTCCAAAGGG TTCTTTGAAGGCTCCATTGCAAGCAAGGGGAAGGGAGGAGCATTGGCTGCTGACATTGACATCGACACTG TAGGCACTGAAGGCTGGGGAGAGGACGCAGAGCTGCAGCTGGATGAAGATGGGTTTGTGGAGGCTCCAGA AGGTTTGGGAGAGGATGTTCTTGGAAAGGGACAAGAAGAAGGAGGTGGCTGGGATGTAGAAGAAGATTTG GAGCTCCCACCTGAGCTGGATGTACCCTCTGGGGTGTCTGGTAGTGCTGAAGATGGGTTCTTTGTGCCCC CAACCAAGGGAACAAGCCCAACGCAAATCTGGTGCAATAACTCCCAGCTTCCAGTAGATCACATCTTGGC AGGTTCTTTTGAAACAGCCATGCGGCTTCTCCATGATCAGGTGGGAGTAATCCAGTTTGGCCCCTATAAG CAACTGTTCCTACAGACATATGCCCGAGGCCGCACCACCTATCAAGCGCTGCCCTGCTTGCCCTCCATGT ATAGTTACCCTAATCGCAATTGGAAGGATGCAGGGCTAAAGAATGGAGTACCAGCTGTAGGCCTAAAGCT TAATGACCTGATCCAACGTTTGCAGCTGTGTTATCAGCTCACCACAGTTGGCAAGTTTGAGGAAGCTGTG GAAAAATTCCGTTCCATCCTACTCAGTGTGCCTCTTCTTGTTGTGGACAATAAACAGGAGATTGCAGAGG CCCAGCAGCTCATCACCATTTGCCGTGAATACATTGTGGGTTTGTGCATGGAGATAGAAAGGAAGAAGCT ACCTAAGGAGACCCTAGACCAGCAGAAGCGCATCTGTGAGATGGCAGCCTACTTCACACACTCAAATCTG CAGCCTGTGCATATGATCTTGGTGCTGCGGACAGCCCTCAACCTTTTCTTCAAGCTCAAGAACTTCAAGA CAGCCGCCACCTTTGCCCGACGCCTGCTGGAACTTGGGCCCAAGCCTGAAGTAGCTCAGCAGACAAGAAA AATCTTATCTGCCTGTGAGAAAAACCCCACAGATGCCTGCCAGCTCAATTATGACATGCACAACCCCTTT GACATTTGTGCTGCATCTTACCGGCCCATCTACCGTGGAAAACCAGTGGAGAAGTGTCCACTCAGTGGGG CCTGCTATTCCCCTGAGTTCAAGGGGCAAATCTGCAGGGTCACTACAGTGACAGAGATTGGCAAAGATGT GATTGGGCTGAGGATCAGTCCTCTTCAGTTTCGCTAAGGCCCTTCCTTCGCGTGCATGGTTAACCCCATA CTGTTCTTCAGAGTCTCCCCTTACAGCCACATTCAGCTTGTTGATGTTGGTTCTCCCTAAAACGTCATGT CCTCTCTCTGCTATTGCAAATCAGAAGTTTCAAGCTTGACTTTCTGGTTCTTGCAGACCTCTGCCTTGAC GGTTACTGTGCTTGTGATCATAGATGACTTTGCCTTTCAGCTTAGGCCTTCCAAGGAACAATGAAAACTA TGTGCTGGCCATTTGTTTATCAGATTCTTAGTATTTACTCCTCCCTTCCCACCACCTTCCTGTCAATTCC AGTACGAGACAGAAGGATTCAATAAACGCCACTTTCCCCAGCCACGCGAGTTGTTAACTATCCCATCTTT TTCTTGGGCATTTAATGTCATAAATAGGTTCCTCCTTTATGTGGCCCCCCACACTTCAGGCTGAGAAGAA GCCATTTGGTTTTGCAAAATGTGTACCATTTTTCATTGATTTGTTTCTATCCTTTATAACATACCATCGT CGTTATGTTCTGTTGCCCTCACTAAATAAAGTAAATTTCTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498656 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498656 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10969 same embryo
	EMAGE:10973 same embryo
	EMAGE:10971 same embryo
	EMAGE:10970 same embryo
	EMAGE:10972 same embryo
	EurExpress:euxassay_003303 same experiment
	MGI:4824002 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source