EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10976

Acsl1 acyl-CoA synthetase long-chain family member 1 ( MGI:102797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001151. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001151_01	euxassay_001151_02	euxassay_001151_03	euxassay_001151_04

euxassay_001151_05	euxassay_001151_06	euxassay_001151_07	euxassay_001151_08	euxassay_001151_09

euxassay_001151_10	euxassay_001151_11	euxassay_001151_12	euxassay_001151_13	euxassay_001151_14

euxassay_001151_15	euxassay_001151_16	euxassay_001151_17	euxassay_001151_18	euxassay_001151_19

euxassay_001151_20	euxassay_001151_21	euxassay_001151_22	euxassay_001151_23	euxassay_001151_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10976_wholemount_strong.wlz

10976_wholemount_moderate.wlz

10976_wholemount_weak.wlz

10976_wholemount_possible.wlz

10976_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10976_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

regional

moderate expression: see section 22 23 24 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

foregut-midgut junction

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

weak

regional

weak expression: see section 06 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19 weak expression: see section 05

nasal capsule

weak

regional

weak expression: see section 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20

anal canal

moderate

regional

moderate expression: see section 13 weak expression: see section 14

pharynx

weak

regional

weak expression: see section 10 11 12 13 14 15 16

esophagus

weak

regional

weak expression: see section 12 13

tongue

weak

regional

weak expression: see section 11 12 13 14 15

stomach

weak

regional

weak expression: see section 03 04 05 06

hindgut

weak

regional

weak expression: see section 13 14

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

weak

regional

weak expression: see section 13 16 18

upper jaw incisor

weak

regional

weak expression: see section 13

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

larynx

moderate

regional

moderate expression: see section 12 13 14

trachea

moderate

regional

moderate expression: see section 13 14 15

chondrocranium

moderate

regional

moderate expression: see section 24 weak expression: see section 02 05 06 07 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4882
Entity Detected:	Acsl1, acyl-CoA synthetase long-chain family member 1 ( MGI:102797)
Sequence:	sense strand is shown >T4882 GTGACAGCCGCCTGTGGAGCAGCCCGGGCAGCGGAGGAGAATTCTGCATAGAGAACCACCAACCCAGAAC CATGGAAGTCCATGAATTGTTCCGGTATTTTCGAATGCCAGAGCTGATTGACATTCGGCAGTACGTGCGC ACCCTTCCAACCAACACCCTCATGGGGTTTGGGGCTTTTGCAGCGCTCACCACCTTCTGGTATGCCACCA GGCCTAAGGCCCTGAAGCCACCATGTGACCTCTCCATGCAGTCAGTGGAAATAGCGGGTACCACTGATGG TATTCGAAGATCAGCAGTCCTTGAAGATGACAAGCTCTTGGTGTACTACTACGACGATGTCAGAACCATG TACGATGGCTTCCAGAGGGGGATTCAGGTGTCAAATAATGGTCCTTGTTTAGGTTCTCGGAAGCCAAACC AGCCCTATGAGTGGATTTCCTACAAAGAGGTGGCAGAACTGGCTGAGTGCATAGGCTCCGGGCTGATCCA GAAGGGGTTCAAGCCTTGCTCCGAGCAGTTCATCGGCCTCTTCTCTCAAAACAGACCCGAGTGGGTGATC GTCGAGCAAGGATGCTTCTCTTACTCAATGGTGGTCGTCCCGCTCTATGACACCCTTGGAGCTGACGCCA TCACCTACATAGTGAACAAAGCTGAACTCTCTGTGATTTTTGCTGACAAGCCAGAAAAAGCCAAACTCTT ATTAGAAGGTGTAGAAAACAAGTTAACACCATGCCTTAAAATCATAGTCATCATGGACTCCTACGGCAGT GATCTGGTGGAACGAGGCAAGAAGTGTGGGGTGGAAATCATCAGCCTCAAAGCTCTGGAGGACCTTGGAA GAGTGAACAGAGTGAAGCCCAAGCCTCCAGAACCCGAAGATCTTGCGATAATTTGTTTCACAAGTGGAAC TACAGGCAACCCCAAAGGAGCAATGATCACTCACCAAAACATTATAAACGACTGCTCAGGTTTTATAAAA GCAACAGAGAGTGCATTCATCGCTTCCACAGATGATGTGCTGATATCTTTCTTGCCTCTCGCCCATATGT TTGAGACCGTTGTAGAGTGTGTAATGCTGTGTCATGGAGCTAAGATAGGATTTTTCCAAGGAGATATCAG GCTGCTTATGGACGACCTCAAGGTGCTTCAGCCCACCATCTTCCCTGTGGTTCCCAGGCTGCTGAACCGG ATGTTCGACAGAATTTTTGGACAAGCAAACACTTCCTTGAAGCGATGGCTGTTGGACTTTGCCTCCAAAA GGAAAGAGGCGGAGCTTCGCAGTGGCATCGTCAGAAACAACAGCCTGTGGGATAAACTCATCTTCCACAA GATACAGTCGAGCCTGGGTGGGAAAGTCCGGCTGATGATCACAGGAGCAGCCCCGGTGTCTGCCACAGTG CTGACGTTTCTGAGGACAGCGCTCGGCTGCCAGTTCTATGAAGGCTACGGACAGACCGAGTGCACTGCTG GTTGCTGCCTGAGCTTGCCCGGAGACTGGACGGCAGGCCATGTTGGAGCCCCCATGCCTTGCAATTATGT AAAGCTTGTGGATGTGGAAGAAATGAATTACCTGGCATCCAAGGGCGAGGGTGAGGTGTGTGTGAAAGGG GCAAATGTGTTCAAAGGCTACTTGAAAGACCCAGCAAGAACAGCTGAAGCCCTGGATAAAGATGGCTGGT TACACACGGGGGACATTGGAAAATGGCTGCCAAATGGCACCTTGAAGATTATCGACAGGAAAAAGCACAT ATTTAAACTAGCCCAAGGAGAGTACATAGCACCAGAAAAGATTGAAAATATCTACCTGCGGAGTGAAGCC GTGGCCCAGGTGTTTGTCCACGGAGAAAGCTTGCAGGCCTTTCTCATAGCAGTTGTGGTACCCGACGTTG AGAGCCTACCGTCCTGGGCACAGAAGAGAGGCTTACAAGGGTCCTTCGAAGAACTGTGCAGGAACAAGGA TATCAATAAAGCTATCCTGGACGACTTGTTGAAACTTGGGAAGGAAGCCGGTCTGAAGCCATTTGAACAG GTCAAAGGCATTGCTGTGCACCCGGAATTATTTTCTATTGACAACGGCCTTCTGACTCCAACACTGAAGG CGAAGAGGCCAGAGCTACGGAACTATTTCAGGTCGCAGATAGATGAACTGTACGCCACCATCAAGATCTA ACGTGAGGAAGGATACTTAGAAGAAATGGCGCATCTCCACAATCCTCCTCGTACCAATGGCCTTCGAGTT GGTAACTTTGCCTGCAGCGAGTGTGGGAAAGGAAATGCCCTGCCGCCGGACTTGTCCACGGGGTCTTACC ATAGGGATAGTCGAGGGCACGGAACACTGCCTTACTTACAGTCACCTGTGTTGCAGCCCATGATCCCGGG GACACACAATTTCCAAAACGAGCCTTAAACATTGTAAAGGGGAACCCATAAAAGTGCTAAGTTATTTAAG ACTTCTTCAACCAATAAGGTGGATGCTACAAGTTCTGTCTCCTGTTTTTCTAACTGAGGGGTTAGGACTT ATTCTTTCTGATATGTCTGCTGCTTGCTGCGCGTTTTGCAGCTGTCTGCTGCTCTGAAGAGCACCGTACA CTGGAGGAAAGCTGTCCCTTTAAGAACAACTGTCCAGGCTGAAGAAAGTCACAGTGGACCAGAGGTTTTC CTTTTGAACCCCTCCTCCCCCTTGCCCCTTTCCCTCACCACCTCACATACAGTACACTCACATGACCTTT CTGGTTTGTAAGGGTTCCACACGGTCCCTGTTTGCGCCTGCTGGAACATGAGGTTTTCAGTAAACTAAAG AGCAGACCCTCTTCAGAACATGTGGGTGTCCAGTCTCAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098645 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098645 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10978 same embryo
	EMAGE:10979 same embryo
	EMAGE:10975 same embryo
	EMAGE:10977 same embryo
	EMAGE:10980 same embryo
	EurExpress:euxassay_001151 same experiment
	MGI:4822938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source