EMAGE mouse gene expression spatial database

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EMAGE:10978

Ugt2b34 UDP glucuronosyltransferase 2 family, polypeptide B34 ( MGI:2140962)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001177. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001177_01	euxassay_001177_02	euxassay_001177_03	euxassay_001177_04

euxassay_001177_05	euxassay_001177_06	euxassay_001177_07	euxassay_001177_08	euxassay_001177_09

euxassay_001177_10	euxassay_001177_11	euxassay_001177_12	euxassay_001177_13	euxassay_001177_14

euxassay_001177_15	euxassay_001177_16	euxassay_001177_17	euxassay_001177_18	euxassay_001177_19

euxassay_001177_20	euxassay_001177_21	euxassay_001177_22	euxassay_001177_23	euxassay_001177_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10978_wholemount_strong.wlz

10978_wholemount_moderate.wlz

10978_wholemount_weak.wlz

10978_wholemount_possible.wlz

10978_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10978_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 17 18 19

nasal capsule

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

anal canal

weak

regional

weak expression: see section 13 14

tongue

weak

regional

weak expression: see section 11 12 13 14 15 16

stomach

weak

regional

weak expression: see section 03 04 05 06 07

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

liver lobe

moderate

graded

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

kidney calyx

weak

regional

weak expression: see section 09 10 11 18 19 20 21

larynx

moderate

regional

moderate expression: see section 11 12 13 14

left lung lobar bronchus epithelium

moderate

regional

moderate expression: see section 09 10 11

trachea

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4881
Entity Detected:	Ugt2b34, UDP glucuronosyltransferase 2 family, polypeptide B34 ( MGI:2140962)
Sequence:	sense strand is shown >T4881 GCCTGAAGTTAACCAAGATGCCTGTGAAAATGACAGCAGCCCTGCTCCTGCTCCTGCTCCAGCTGAGTGG CTTCTTTGGATCTGGGACTGGTGGGAAGGTGCTGGTGTGGCCAATGGAGTTCAGCCATTGGCTCAACTTG AAGACAATCCTAGACGAGCTTCTGAAGAAGGGTCATGAAGTGATGGTTCTGAGACCTTCAGCATCCTTAT CTTATGAGGTTGACAACACATCTGCTATTGAATTCGAGACTTATCCTACATCATACTCCTTATCTGAGTT AGAGGAAATTTTCTGGGAATCACTCAAGAAATATATTTATGAGCTGCCAAAGCAGTCATTTTGGGGATAC TTCTTAATGTTGCAAGAAATGGTTTGGGTAGATTCAAAATATTTTGAAAGTCTCTGTAAAGATGTAGTTT TTAACAAAGAACTCATGACAAAGCTACAAAAATCAAGGTTTGATGTGATCCTGGCAGATCCCTTCATACC CTGCGGTGATCTGCTGGCTGAGGTTCTCAAGATCCCCCTTGTGTACAGTCTCCGCTTCTTTCCTGGCTCC ACTTATGAGAAGTACAGTGGGGGACTCCCACTGCCTCCTTCCTATGTGCCTGTTGTTATGTCAGAATTGA GTGACAGGATGACTTTCATGGAGAGGGTGAGAAATGTCATCTATATGCTGTGTTTTGACTTTTGGTTCCA AACATTTAATGAGAAGAACTGGAATCAGCTTTATACTGAAGTATTAGGAAGACCCACGACACTCTCAGAG ACTATGGCCAAAGCAGACATATGGCTCATTAGGACCTACTGGGATTTAGAATTTCCTCATCCTGTCTTAC CAAATTTTGATTTTATTGGTGGCCTCCACTGCAGACCTGCCAAGCCCCTGCCTAAGGAAATAGAAGACTT TGTCCAGTCATCTGGAGAACATGGTGTTGTGGTGTTTTCCCTGGGGTCCATGGTGGGTAGCATAACAGAA GAAAGAGCCAATGTGATTGCAGCAGGCCTTGCTCAGATTCCACAGAAGGTTCTGTGGAGATTTGAGGGCA AGAAGCCAGAGACATTGGGCTCCAACACTCGTCTGTACAAATGGATCCCCCAGAATGACCTTCTAGGTCA TTCCAAAACCAGAGCGTTTATAACTCATGGTGGCACCAATGGCATCTATGAGGCGATCTACCATGGGATT CCAGTGGTTGGCATTCCTTTGTTTGGAGACCAATATGACAATATTGTTCACTTGAAGGCCAAAGGAGCAG CTGTTAGACTGGACTTTCTCACAATGTCTAGTACAGATTTGCACACTGCACTGAAGACAGTCACTAATGA CCCTTCCTATAAGGAGAATGCCATGCGGTTATCAAGAATTCACCATGATCAGCCAGTGAAGCCCCTGGAC CGAGCAGTCTTCTGGATTGAGTTTGTCATGCGTCACAAAGGAGCCAAGCATCTTCGTGTGGCAGCCCATG ACCTCAGCTGGGTCCAATACCACTCTCTGGATGTGCTTGGGTTCCTGCTGGCCTGTGTGCTGACTGTGAT GTTCATCCTCAAAAAGTGTTGCCTCTTTTGTTGCCAGAAGTTGACTAAAGCTGGAAGGAAGAAGAAAGGG GAGTAGCTGAACTGGGGAAGACTGACACATGGTCCTTTCCAGTGCATCACAGAAAGGGAGGCAATGATTT AGGGTCCTTCATCCTGTGACAAGATAGTATTTCAATAATTACTTATTAAGTCAGAAGATATTTTTTTTCT CCGAGAGATTTAATAATTCTTTTTGAAATTAGTAGTATTTCAGCCAAATATAAAATTGAAAGGGAAACAC AGTAAAATTAACACCATCAACATGAAACGGAAACAACAGACATTTTACAAATTCATTTCTGCAACATCAA AGTTAAACCAAAGAATGTTACATCTGAAGAGCTGGCTCCATGGTGCAACTATTTGTCAGGCAAGACTTCC TCCTTGATTTTTGAGGAACTCATAAAAAATCCATATCAAGTAGTGTCTATAACTCTAGTATGAGAAAACC AAGAGATGTGAAACAGACACATGAGAATTACCTGAAGCTCTCAGGCCAACTAGCCTGGTCTTCAGCAAGA AATAGCCTGCCTCATAGAATGTGGAAGGGAAACTAACACTGAAAGCTGTGCTTTGACATCTGAACATTTG TGCCATGGCATAGGTAAGACCCACTCACAAATTCATAAGCATAAGGATATAATTATACATATGTGTTCAG GCTTTATATAAACACACACACACATATAGACAACACACCACTTATAACTCATAGTTTGTTTGCGGATATA CCATGAGTAAGAAGCCCACAATTTCTACATGGTTTTGCAAGTATTGAACAAGCTTTAACTTTATTTATTG CAGACTTTACAGGATGAATATCTAGGTAACATGTTCAGGATTGGAGCAGTGTGAAACCTGTAACCCTACA TTTGTCTTTAGAACACTTGTACTTTTGCAGACTCTCATGTGGTGCAATAAGCAATAAGCAATGACCTCTT CCTTAAAGGAACTACGAATATTGGACTTGGGGCTGGGGAGATGGCTCAGCAGTTACTAACTAACTCTTCT TCTAGAGAACCCATATTCCATTCCTATCACCCATATGGTGATGATTCAGGACCAGCAGTAACTCAAGTGT CACTATGGGATCTTACACCTTCTTCTGGCCTCTCTTGGTACTGCAGAGACACATGCAAAGGAAGCCTGCA TGCACATAAAATACAATTTAGGATTAAAGACAATTTTTCATTTCATGAAATGAATTCTATTTCCATTCAA CCCTTGACAAAGAATAATGAAATTCCTTCCTTTTTATAATTAGCTTTTGGGAACAGATGTAATATTTTAA TTTATGGTTTGATAACACAGAATAAAGACTCTAAATAAGTTATTCTCACACAAGTGCAGGATGTGTGTAT GTATGTGATTCATGTCCCTTTTTATAAAACAGAATGTACTTTACCTATGCCCTCTATATTTATGCTGTGA CTTCAATATTTCTAAAAGAAATAAATGTTTTGAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098890 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098890 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10976 same embryo
	EMAGE:10979 same embryo
	EMAGE:10975 same embryo
	EMAGE:10977 same embryo
	EMAGE:10980 same embryo
	EurExpress:euxassay_001177 same experiment
	MGI:4829089 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source