EMAGE mouse gene expression spatial database

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EMAGE:10979

Uroc1 urocanase domain containing 1 ( MGI:2385332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001178. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001178_01	euxassay_001178_02	euxassay_001178_03	euxassay_001178_04

euxassay_001178_05	euxassay_001178_06	euxassay_001178_07	euxassay_001178_08	euxassay_001178_09

euxassay_001178_10	euxassay_001178_11	euxassay_001178_12	euxassay_001178_13	euxassay_001178_14

euxassay_001178_15	euxassay_001178_16	euxassay_001178_17	euxassay_001178_18	euxassay_001178_19

euxassay_001178_20	euxassay_001178_21	euxassay_001178_22	euxassay_001178_23	euxassay_001178_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10979_wholemount_strong.wlz

10979_wholemount_moderate.wlz

10979_wholemount_weak.wlz

10979_wholemount_possible.wlz

10979_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10979_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4880
Entity Detected:	Uroc1, urocanase domain containing 1 ( MGI:2385332)
Sequence:	sense strand is shown >T4880 AGCTTCTGCAGACATGTCCAGCCTCCAGGAACTGTGCTCTGGTCTGCCCCTACGACCCCTTCCTGAGAAC CGGGGTCGCTGGGCTGGGGTGCCACATGCTCCGGTTAGAACTCCGAACCTCAGCCCTGAAGAAGAGCAGC TGGCCCTGAGGAATGCCTTGCGCTATTTCCCACCTGATGTCCAGAAACTGTTGGCCCTGGAGTTTGCCCA GGAGCTAAGACAGTTTGGACACATCTACATGTACCGGTTCTGCCCCAGCATTGAGATGAGAGCCTACCCA ATTGATCAGTACCCATGCCGGACACGGGCAGCTGCAGCCATCATGCACATGATCATGAACAACTTGGACC CTGCTGTGGCCCAGTTTCCACAGGAGCTGGTGACCTACGGAGGCAACGGGCAAGTGTTCAGCAACTGGGC CCAGTTCCGGCTGACCATGTCCTACCTGTCGAAGATGACTGAGGAACAGACTCTGGTCATGTACAGTGGA CACCCTCTGGGCCTCTTCCCCAGCAGCCCCCGTGCCCCACGGCTGGTCATCACTAACGGGATGGTCATTC CCAACTACTCCTCCAGGACAGAGTATGAGAAGCTGTTTGCCTTGGGGGTGACCATGTATGGCCAGATGAC AGCGGGCAGCTACTGCTACATTGGTCCCCAGGGAATCGTCCATGGCACAGTGCTCACTGTGCTGAATGCT GGTCGTCGGTACCTGGGCATCGAGAACTTGGCAGGGAAGGTCTTTGTCACCTCTGGGCTTGGCGGGATGA GTGGGGCTCAAGCCAAGGCAGCAGCCATCGTTGGATGCATTGGGGTGATTGCGGAGGTGGATAAAGCTGC ACTTGTAAAACGCCACCGGCAAGGCTGGCTGATGGAGGTGACAGACAGCTTGGACCACTGCATTGCAAGA CTCAGGGAAGCAAGGAAAAAGAAGGAGGTTCTCAGCCTCGGCTACCATGGCAACGTGGTGGATCTTTGGG AGCGCCTTGTCCGTGAACTGGACACAACGGGGGAACTCTTGGTGGACCTCGGGTCAGACCAGACATCCTG TCACAACCCGTTCAATGGCGGCTACTACCCTGTGCAGCTCAGCTTCTCAGAGGCCCAGAGCCTCATGTCC TCCAACCCTGCTGCCTTCAAGCACCTGGTGCAGGAAAGCCTGAGGAGGCATGTCGCAGCCATCAACAGGT TGGCCCAAGAGAAGTTCTTCTTCTGGGACTATGGTAATGCCTTCCTCTTGGAGGCTCAGAGAGCAGGAGC AGATGTAGAGAAGAAAGGAGCCAACAAGATGGAGTTCCGCTACCCCTCCTATGTCCAGCACATTATGGGG GATATATTCTCCCAGGGCTTTGGGCCCTTCCGCTGGGTATGCACATCAGGGGACCCCCAGGACCTGGCTG TCACTGATCATCTGGCCACATCTGTACTGGAGAAAGCCATTGCTGATGGAGTGAAGGCATCTGTGAAGCT ACAGTATATGGATAATATACGCTGGATCCGAGAGGCTGCCAAGCATCAGCTGGTGGTGGGCTCCCAGGCA AGGATCCTGTACTCAGACCAGAAAGGCCGTGTGGCCATTGCTGTGGCCATTAACCAGGCCATCGCCAGTG GGAAGATCAAGGCACCAGTGGTCCTGAGCCGTGACCACCATGATGTGAGTGGCACTGACAGCCCCTTCAG GGAGACTTCGAATATTTATGACGGTTCTGCCTTCTGCGCAGACATGGCCGTGCAGAACTTCGTGGGAGAT GCCTGTCGTGGTGCCACCTGGGTTGCACTTCACAATGGAGGGGGTGTAGGCTGGGGTGAAGTCATCAATG GGGGATTTGGCCTTGTTCTAGATGGAACTGCAGAGGCTGAGCAGAAAGCCAGGATGATGCTCAGCTGGGA TGTCTCCAACGGTGTGGCACGACGCTGCTGGTCTGGAAACCCCAAGGCCTATGAGATCATCTGCCAGACA ATGCAGGAGAACAGTGGCCTGGTGGTGACGCTTCCCCATGAGGTGGCAGATGAACAAGTGCTACAGCAGG CCCTGCGGCCCTGAGAAGAGCAAGGGTCCTCTTGATCCCCTCCTTCCCACTGCCCTCCCCTCGCAGCTCA TGACCCTCCCCCACCCCACTGCATCCCTACATGGCACTCTTCGTGTCCCACATACTAACCACAGAGGCCT ATCAAGGTGAAGGCGCTAGTCTTTGACCACTGGTTTGAGGGCAGGGTACATCTTGTTCATGAACAGGCCT GTAAGAGTAGACTCCAGCTTGGATAGGAAGGAACCTTGAATGCTGGCCAAGCTGGCACCCGCCTCACAAG ATTCTCTGTGACCTTCTAAATGTGCTGTTCAGTCTCCTATCTACTACTGTCCACGCAGTTACCCAGCCAT CCCTTGTCTATGTCATCCATCACTTCCGGTTCCTCAGAGAACCTGGCATTCTCTCATGGGTTCACAAAGT GCTCACACCAAGTCCTTGGTGCTGAGGAGTATGGGGGAAGATAGACAGGAAATAAAGACTAGAGTAGAGC CAAACACATGGCACAGACTGGGCTAGCAAGAGTGGATAACAGAGTCTCTGAGGCAAAGTGGCCAGGTGCT GCTGCAACATGTGGAGAAAAGGCAATTGTGTGACCATGAAAAACAGAACCAACCACTTCCAGAGGTCCCT GGGTGGATCTGGAATCCTGCAGGAGTTGCATTAGCCATAAGCTGGGTTCTTATATCTTTCCCATGGCCTG CAATCCAACCTCTGAAGCAGGAGACCTAGCAAGAAAGTTGGGTAGCTGTGCTTCCTCCCCCTCCCCCTGG AAGTTGCCACTACTTTATAATGCCTCCTAACCCCCAATACCCATCTTTTAGGCCCCTTCCCACTACCATG TCCTGTGCCCTCTTTTCAATCTTCACCAGGCCAGATCTCCTGCTGTGTGATCCCCTAAACTGAGGCAGGC CTACAAGGCAGTTAACTTACAAAGAGAAAAAGGATTTTTTTTTTTTTTTTTTTTTTTTGGCTGACCATTC TGGATGTTCCCACGCAAGATAAGGAAGTCGTGTTGCTTTGGGCCTCTGGCAACAATGCCAAGTGGCAATG GCAGGGAGCACATGGCAGAGCAAAATTACACTATGAGCCAGGAAGAAAGAAGAGACGCTTGGACCCAAGT CCTCCAATCCTTCAAGGGTGTGTTCCAGTAGCCTAAAGCCCTTTCCCTAGCTCCACTTCTAAAGGTCCCC TCACCTCCCTGCACAGCTTTGGAATAAGCCTTTAGCCCATCTGCAGCCATAGCAGAGGTCAAAATCGGCT TTCTGGCATTTTGGTCTATGTTTCTTGCATTAAATAAAGATTTTCCTAAATCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098655 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098655 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10976 same embryo
	EMAGE:10978 same embryo
	EMAGE:10975 same embryo
	EMAGE:10977 same embryo
	EMAGE:10980 same embryo
	EurExpress:euxassay_001178 same experiment
	MGI:4829120 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source