EMAGE mouse gene expression spatial database

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EMAGE:10980

Tfcp2 transcription factor CP2 ( MGI:98509)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003118. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003118_01	euxassay_003118_02	euxassay_003118_03	euxassay_003118_04

euxassay_003118_05	euxassay_003118_06	euxassay_003118_07	euxassay_003118_08	euxassay_003118_09

euxassay_003118_10	euxassay_003118_11	euxassay_003118_12	euxassay_003118_13	euxassay_003118_14

euxassay_003118_15	euxassay_003118_16	euxassay_003118_17	euxassay_003118_18	euxassay_003118_19

euxassay_003118_20	euxassay_003118_21	euxassay_003118_22	euxassay_003118_23	euxassay_003118_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10980_wholemount_strong.wlz

10980_wholemount_moderate.wlz

10980_wholemount_weak.wlz

10980_wholemount_possible.wlz

10980_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10980_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4877
Entity Detected:	Tfcp2, transcription factor CP2 ( MGI:98509)
Sequence:	sense strand is shown >T4877 CTTTCGCTGTCCACGCTTCCAGGCGGTCGTGGTGGCTAAGGAGGGTGTGGGGACGGCTCGGAGGAACCCT GGGAGCCGGCGCCTGTTGGGGCAAGGAAGGAGCCGGGATGGCCTGGGCTCTGAAGCTGCCGCTAGCCGAC GAAGTGATCGAGTCGGGGCTTGTGCAGGACTTTGATGCTAGCCTGTCCGGCATTGGCCAGGAACTGGGTG CTGGTGCTTATAGTATGAGTGATGTCCTCGCATTGCCCATTTTTAAGCAAGAAGAGTCAAGTTTGCCTCC CGATAATGAGAACGAGATTCTGCCTTTTCAGTATGTGCTCTGTGCTGCCACGTCTCCAGCGGTGAAGCTC CACGACGAGACCCTGACGTACCTCAATCAAGGACAGTCTTATGAAATCCGAATGCTAGACAATAGAAAAC TGGGAGAGCTTCCAGAACTCAACGGCAAGCTGGTGAAGAGCATATTCCGCGTAGTGTTCCACGACAGGCG ACTGCAGTACACTGAGCACCAGCAGCTGGAGGGCTGGAGGTGGAACCGACCGGGAGACAGGATTCTCGAC ATAGATATCCCAATGTCTGTGGGTGTAATTGATCCCAGGGCCAATCCTACCCAGCTAAACACAGTGGAGT TCCTGTGGGACCCTTCAAAGAGGACATCTGTGTTTATTCAGGTGCACTGCATCAGCACTGAGTTCACCAT GAGGAAGCATGGCGGGGAGAAGGGGGTGCCGTTCCGAGTGCAGATCGATACCTTCAAGGAGAACGGGAAC GGAGAGTACACAGAGCACTTGCACTCGGCCAGCTGCCAGATCAAAGTCTTCAAGCCTAAGGGTGCAGACA GAAAACAAAAAATAGATAGAGAGAAAATGGAGAAACGGACGCCCCATGAAAAAGAGAAATATCAACCTTC TTATGAGACAACCATACTCACCGAGTGTTCTCCGTGGCCTGAGATCACCTACGTCAACAATTCCCCATCC CCTGGCTTCAACAGCTCCCATAGCAGTTTCTCTCTTGGGGAAGGAAACGGCTCGCCCAACCACCAGCCAG AACCACCCCCTCCAGTCACGGATAACCTCTTGCCGACAACCACGCCTCAGGAAGCCCAGCAATGGCTGCA TCGAAACCGGTTCTCCACATTCACGAGGCTTTTCACCAACTTCTCAGATTTACTGAAACTAACTAGAGAC GACGTGATCCAAATCTGCGGCCCTGCAGATGGAATCAGACTCTTTAATGCATTAAAAGGCCGGATGGTGC GGCCAAGGCTAACCATTTATGTCTGCCAGGAGTCGTTGCAGTTGAGGGAGCAGCAGCCGCAGCCGCAGCC GCAGCCGCAGAAGCAGGAGGATGGGGATTCAAACGGTACTTTCTTCGTGTACCATGCTATCTATCTCGAG GAGCTGACAGCTGTCGAACTCACAGAAAAAATTGCTCAGCTTTTCAGCATTTCTCCTCACCAGATCAGCC AGATTTACAAGCAAGGGCCGACAGGGATCCATGTGGTCATCAGCGATGAGATGGTACAGAACTTCCAGGA AGAAGCCTGCTTTATTCTGGACACGATGGAAGCAGAAACCAGTGACAGCTATCATGTCATTCTCAAGTAG GGTGAGGCCCCGACCTTCGACGGCTGCTGCTGCCTTCCCCTCTTCACCTTCTGAAGGGGACATGACTGGA GATTAAAGGGCTTCAGGAACCTGGCACATCTGAATGTTGGGGGGAGGGGGGAGGCGTGTCCAGTGCAGGG CGAGCAGGTCCCAAGGAGGAATCCCAGAGTCCTGTATTGGCTCCTCTGATCACTGGCTATACATGGAGCC AGCAGTTTCCTTACTTTCTGGCCCCTGTTGATACCAGTAAGAAGCCCCGTCATTAGGGTTTGTAGAGGGC AGAGTGGTAATGGGAGTCATGTGATGTTTTCTAGGAAGATGTTTAGTTTTGTGATATAGAGCTCTGACAT TTTACTGATATAAAAGTGAACATCTGTCTTCATAAAACCAAGCTCTCTCTCTTGCCTCAAGCCTGTTGGC AGCTCTGCTCTGCTCTGTTCACAACTGTGAGGAGGAGAAACAGAATCTCTCCTTCCCTTTTTTGAGACAG GGTTTCATGTTCTTGCCTCTACCTCCCAAGAGCTGGGATCACAAGCTTTTGCCACCTGGTCTGTTTCTGT CTGCAGTGCTGGGCTTGAACTCGGAGCCTCACGTGCTAGGCAGGCACTTTCTCCAGCTGAGCTCCAGCAG GCCTTCCCTTTAGTGCTAAGACAGGCTCCAGATTTCTAATTTGGTTCACCTGCAATACCACAGCTGAGTG TGATTTTGGCTAATGTGTATTGGGGTAGACCGAATGATAAATCCCACGGACGGATAATCTTCTTTTATCC CCTTAATAAACCCAAAACATCTGGGGACACAGTGACTTGAGGGTGAGGAGACGCAGATGAAGTGGAGGGG CTTCTGACAACACCAGATGCCACCCTTTGCTGCCAGTGCTCACCACTGGGTCTTTCTGGGGCTCTGCTGG AAACTGACGGACTTCCCACTGAAGGAGGCAACCATACCTTACCACTGTAGCGATAATGAAGCCCCAGACA GACAGACACCCAATGTCACACTAACCTCAGCCCAGAGCTAGAGGTGAGAGAGACAAGCGTGTCTGACCCC AGGCTTCGTTGTTCGTGAATGATATTCTCAAAACTGTTTAAGTAAATGAGCTATTCCGCCCTGCTCTCCC CTCCCCTGTCTTGTTTTGGCATAGTGTGGTGTTTTTTTTTTTTTTTTTTGTTCTTTTTTTAAAATGTAAG CTTTATTAAAAAAAGAAAAATACATTCTTTTCCTATTCAAACTCACACTGTGTTTATGTTCTGCCTTTCA AATGGCTGTGTGTCTGTGTGCATGTACAGAATTGTAAAGCACTTAGGCCCTCCTTTTTCACATGAAGTTT GTTTTAGCTTGGTTTTCCAAGAAAACGTTTCTCTGTGGAGCCCCGGCTGTCCTGGAACTCACTCTGTAGA CCAGGCTGGCCTCAAACTCAGAAATCCACCTTCCAAGTGCTGGGAATATAGGTGTGTGCCACTAGCTTAG TCAGGCCACATGTAAAGTTTTACCAGAAATTCCCTTAAATTTTGTTGAGATGAACAAAGATAATGTTAAA GATTCTGTATTAAATTTGAATTTATGTGTGTTTTTGAAGTATTTAATAAAGCTTAGATTTTTTTATAGAG AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098336 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098336 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10976 same embryo
	EMAGE:10978 same embryo
	EMAGE:10979 same embryo
	EMAGE:10975 same embryo
	EMAGE:10977 same embryo
	EurExpress:euxassay_003118 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source