EMAGE mouse gene expression spatial database

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EMAGE:10988

Mmp8 matrix metallopeptidase 8 ( MGI:1202395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001201. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001201_01	euxassay_001201_02	euxassay_001201_03	euxassay_001201_04

euxassay_001201_05	euxassay_001201_06	euxassay_001201_07	euxassay_001201_08	euxassay_001201_09

euxassay_001201_10	euxassay_001201_11	euxassay_001201_12	euxassay_001201_13	euxassay_001201_14

euxassay_001201_15	euxassay_001201_16	euxassay_001201_17	euxassay_001201_18	euxassay_001201_19

euxassay_001201_20	euxassay_001201_21	euxassay_001201_22	euxassay_001201_23	euxassay_001201_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10988_wholemount_strong.wlz

10988_wholemount_moderate.wlz

10988_wholemount_weak.wlz

10988_wholemount_possible.wlz

10988_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10988_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 01 02 03 04 05 06 21 22 23 24

femur

weak

regional

weak expression: see section 09 10 19 20

nasal capsule

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lower jaw incisor

weak

regional

weak expression: see section 15 16

testis

moderate

regional

moderate expression: see section 06 07 08 09 21 22 23 24

axial skeleton

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4707
Entity Detected:	Mmp8, matrix metallopeptidase 8 ( MGI:1202395)
Sequence:	sense strand is shown >T4707 CAGTGATTGGCAGGGGATGCTGTGAGCTTGAGGGCACTTGCCAGAGGGGCAGAGGAAGGCAGGAGAGGTT GTCTTGGAAGATAAGATTATGTTTCGCCTGAAGACACTTCCATTACTGATCTTCCTCCACACACAGCTTG CCAATGCCTTCCCAGTACCTGAACACCTGGAAGAGAAAAATATAAAAACTGCTGAGAATTACCTACGAAA ATTCTACAACTTACCAAGCAATCAATTCCGGTCTTCGAGGAATGCCACGATGGTTGCAGAGAAGCTTAAA GAGATGCAGCGCTTCTTCAGCTTAGCAGAGACTGGGAAGTTAGATGCAGCTACAATGGGGATTATGGAAA TGCCTCGATGTGGAGTGCCTGACTCTGGTGATTTCTTGCTAACCCCAGGAAGTCCCAAGTGGACACACAC TAACCTGACCTACAGGATTATAAACCATACGCCACAGCTGTCAAGGGCTGAAGTGAAAACAGCAATTGAG AAAGCTTTTCACGTCTGGAGTGTAGCATCGCCCCTGACCTTCACCGAGATCTTACAGGGAGAAGCAGACA TCAACATTGCTTTCGTCTCAAGAGACCATGGTGACAATTCTCCATTTGATGGACCCAATGGAATCCTTGC CCATGCCTTTCAACCAGGCCAAGGTATTGGAGGAGATGCTCATTTTGATTCAGAAGAAACGTGGACTCAA GATTCCAAAAATTACAACCTATTTCTCGTGGCTGCTCATGAATTTGGACATTCTTTGGGACTCTCTCACT CCACTGATCCTGGTGCCTTGATGTACCCAAACTATGCTTACAGGGAACCCAGCACCTATTCACTACCTCA AGATGATATCAATGGCATTCAGACAATCTATGGACCTTCAGACAACCCCATCCAACCTACTGGGCCCAGC ACACCCAAAGCCTGTGACCCCCACCTGAGATTTGATGCTACTACCACACTCCGTGGGGAGATTTACTTCT TTAAAGACAAGTACTTCTGGAGACGGCATCCTCAGCTGAGAACAGTTGACCTCAATTTCATATCTCTGTT CTGGCCCTTCCTACCCAACGGTCTTCAGGCTGCTTATGAAGATTTTGACAGAGACCTAGTTTTCCTATTT AAAGGCAGACAGTACTGGGCTCTAAGTGGCTATGACCTGCAGCAAGGTTACCCCAGAGATATATCCAACT ATGGATTCCCAAGGAGTGTCCAAGCCATTGATGCAGCTGTTTCCTATAACGGGAAGACATACTTCTTCAT AAACAACCAGTGCTGGAGATATGACAATCAAAGAAGATCCATGGACCCAGGTTACCCCAAAAGCATACCA AGCATGTTCCCAGGAGTAAACTGTAGAGTCGATGCAGTTTTCCTGCAGGACTCCTTCTTCCTCTTCTTCA GTGGACCACAATATTTTGCATTTAATTTTGTCAGTCACAGAGTCACTAGAGTTGCAAGAAGCAATTTATG GCTTAACTGTTCATAGAGTTGAGCAATGTCAAATGTCCTTGCACGCACCCTATGAGGACAAAAAGGGAAG CTCAGTCTGTATACTCATCCCACGTGTCCTGCCCAGACTTTCCTCAGTGGTAACTAACTCTGCAGCCCTC TTCGAAGAACCAGCGTTTGTAATCTGGCATTCCCAGCCATGGCTAACTCCTTCTGCATATTCCGTCCCTG GTTCGTGAATGCATCACAAAGAGAGCAAGCCGTTTACTACCTTACTCTTCACTATTTCATTATCTGCTCT TCTGCCTTCTAAAACCTTTTGTTTTATTACTTGCTTATCTAGCTTCAAATGTTCCTAAATCTCTTCAAAC AGGGAATCTAGCATTGGATTTTCATTACAAATGAAGATTATAAACACATAGATATTATCCCCTTATCTGT ACAGAAAAAAAGTGCTGTTATTTCCTCCGTTGGAAAAATATGAAAAATATGTAATGTGTGTTGAATCATG CCCCTCTGCATGCCAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5032770 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5032770 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10987 same embryo
	EMAGE:10990 same embryo
	EMAGE:10986 same embryo
	EMAGE:10985 same embryo
	EMAGE:10989 same embryo
	EurExpress:euxassay_001201 same experiment
	MGI:4826413 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source