EMAGE mouse gene expression spatial database

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EMAGE:10989

Jmjd6 jumonji domain containing 6 ( MGI:1858910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001256. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001256_01	euxassay_001256_02	euxassay_001256_03	euxassay_001256_04

euxassay_001256_05	euxassay_001256_06	euxassay_001256_07	euxassay_001256_08	euxassay_001256_09

euxassay_001256_10	euxassay_001256_11	euxassay_001256_12	euxassay_001256_13	euxassay_001256_14

euxassay_001256_15	euxassay_001256_16	euxassay_001256_17	euxassay_001256_18	euxassay_001256_19

euxassay_001256_20	euxassay_001256_21	euxassay_001256_22	euxassay_001256_23	euxassay_001256_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10989_wholemount_strong.wlz

10989_wholemount_moderate.wlz

10989_wholemount_weak.wlz

10989_wholemount_possible.wlz

10989_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10989_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 19 20 weak expression: see section 09 10 11 18 21

vibrissa

weak

regional

weak expression: see section 07 08 23 24

nasal capsule

weak

regional

weak expression: see section 09 10 11 12 14 15 18 19 20 21

lower jaw incisor

moderate

spotted

moderate expression: see section 14 15 16 18 weak expression: see section 08 13 17

lower jaw molar

weak

spotted

weak expression: see section 20 21

upper jaw incisor

moderate

spotted

moderate expression: see section 14 15 18 19 weak expression: see section 08 13

upper jaw molar

weak

spotted

weak expression: see section 20 21

axial skeleton

weak

regional

weak expression: see section 12 13 14 15 16 17

chondrocranium

moderate

regional

moderate expression: see section 06 07 weak expression: see section 02 03 04 05 08 09 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4708
Entity Detected:	Jmjd6, jumonji domain containing 6 ( MGI:1858910)
Sequence:	sense strand is shown >T4708 GGCGGGTCTGGCAGGTCGCTGAGGCCAATGGCGGACCGCATGAGGCGGTGGGGCGGGGAGGAAGGCCGCT GTTCGCCCAACCCAAAGCTCCGGTGGCGGCTGAGGAGTGAATCTTCTGGAAGGCGACTTTAAAGGCGCCG GGCCGGAGCGAAGGGCGTCTCGGGGCCGCGCCAGCCAGGGAGGGAGGGCGCGTTTGGTGGCAGGAAACGC GAGCCGCGTCGGGGTCGGGGCGGAACCGGACGGCGAGCGGCTCACGATGAACCACAAGAGCAAGAAGCGC ATCCGCGAGGCCAAGCGAAGTGCGCGGCCGGAGCTCAAGGACTCGCTCGACTGGACCCGGCACAACTACT ACGAGAGCTACCCGCTGAACCCCGCGGCCGTGTCGGATAACGTGGAGAGAGCTGATGCCTTACAGCTGTC GGTGAAAGAGTTCGTGGAGCGCTACGAGAGGCCTTACAAGCCCGTGGTTCTGCTCAATGCACAAGAGGGC TGGTCCGCACAGGAGAAATGGACTCTGGAGCGCCTCAAAAGGAAATACCGGAACCAGAAGTTCAAGTGCG GCGAGGATAATGACGGCTACTCGGTGAAGATGAAGATGAAGTACTACATCGAGTACATGGAGAGCACCCG CGATGACAGTCCCCTTTACATCTTCGATAGCAGCTATGGCGAACACCCCAAAAGAAGGAAACTTTTGGAA GACTACAAGGTGCCCAAGTTTTTCACAGATGATCTTTTCCAATACGCGGGGGAGAAACGCAGACCCCCTT ACAGGTGGTTTGTGATGGGGCCACCGCGTTCTGGAACTGGGATTCACATCGACCCTCTGGGGACCAGTGC CTGGAATGCCTTAGTTCAGGGTCACAAGCGGTGGTGCCTCTTCCCAACAAACACACCCAGAGAACTCATC AAGGTGACCCGAGAAGAAGGAGGGAACCAACAGGATGAAGCAATTACCTGGTTTAATGTCATCTATCCCC GGACACAGCTTCCAACCTGGCCACCTGAATTCAAACCCCTGGAGATATTACAGAAACCAGGAGAAACTGT CTTTGTACCAGGGGGCTGGTGGCATGTTGTCCTCAACCTTGACACCACCATTGCCATCACCCAGAACTTT GCCAGCAGCACCAACTTCCCTGTTGTGTGGCACAAGACGGTAAGAGGGAGACCAAAGTTATCAAGGAAGT GGTATAGGATCTTGAAACAGGAGCACCCTGAGCTGGCAGTCCTGGCCGACGCAGTTGACCTCCAGGAGTC CACAGGCATTGCCTCTGACAGCTCCAGCGACTCTTCTAGCTCCTCTAGTTCCAGCTCGTCAGACTCGGAC TCAGAGTGTGAATCTGGGTCAGAAGGTGATGGGACGACACACCGCAGGAAGAAGAGGAGAACCTGCAGCA TGGTGGGAAATGGGGACACTACCTCCCAGGATGACTGTGTGAGCAAAGAGCGCAGCTCCTCCAGGTGACC ACATGTGGCCGCTGTCTGTGCCAGGATATGCTCGCAGCCAGGCTTGGGGGGCTGACACCCAGAGAACAAG GACACTCAGATGGGAGTTGGTAGTACGTGGTACAGTGGCTTTTCTCTGTGGTCATGTCTTAGGGGCAAAG GCGTGTGGATGGTGCCAATGAAACCCTTTACCTATTTAATAAAAACGGATACATTTTAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5033487 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5033487 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10987 same embryo
	EMAGE:10990 same embryo
	EMAGE:10988 same embryo
	EMAGE:10986 same embryo
	EMAGE:10985 same embryo
	EurExpress:euxassay_001256 same experiment
	MGI:4825682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source