EMAGE mouse gene expression spatial database

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EMAGE:11045

Marveld2 MARVEL (membrane-associating) domain containing 2 ( MGI:2446166)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001109. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001109_01	euxassay_001109_02	euxassay_001109_03	euxassay_001109_04

euxassay_001109_05	euxassay_001109_06	euxassay_001109_07	euxassay_001109_08	euxassay_001109_09

euxassay_001109_10	euxassay_001109_11	euxassay_001109_12	euxassay_001109_13	euxassay_001109_14

euxassay_001109_15	euxassay_001109_16	euxassay_001109_17	euxassay_001109_18	euxassay_001109_19

euxassay_001109_20	euxassay_001109_21	euxassay_001109_22	euxassay_001109_23	euxassay_001109_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11045_wholemount_strong.wlz

11045_wholemount_moderate.wlz

11045_wholemount_weak.wlz

11045_wholemount_possible.wlz

11045_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11045_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

weak

homogeneous

weak expression: see section 12 13 14 15 16 17 18 19 20

pancreas

weak

homogeneous

weak expression: see section 10 11 12

nasal capsule

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 21

stomach

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11

hindgut

weak

homogeneous

weak expression: see section 14 15 16 17

midgut

weak

homogeneous

weak expression: see section 10 11 12 13 14 15 16 17 18 19 20

bladder

weak

regional

weak expression: see section 13 14 15

kidney calyx

weak

homogeneous

weak expression: see section 17 not examined expression: see section 08 09 11 18 19

kidney pelvis

weak

homogeneous

weak expression: see section 18 19 not examined expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4848
Entity Detected:	Marveld2, MARVEL (membrane-associating) domain containing 2 ( MGI:2446166)
Sequence:	sense strand is shown >T4848 GGAAAATGTGTGAAGCTGCCATCAGTGATCGACAGAGGGATCAGGAAGTTAATGTCAAAGACTTGAGAAC AACAACTAAAATGACTCCTGAGCTGTTGAGTGGGCACATCCCTCCCGGCCACATTCCGAAGCCTATCGTG ATGCCTGACTACGTGGCAAAATATCCTGTGATTCAGACAGATGACGATCGAGAACGCTATAAGGCTGTGT TCCAAGACCAGTTTTCAGAGTACAAAGAGCTGTCTGCGGAAGTGCAGGCCATCCTGAGGAAGTTTGACGA GCTGGACACCGTGATGAGCAGGCTCCCACATCATTCTGAAAACCGCCAGGAACACGAGAGAATTTCAAGA ATTCATGAAGAGTTTAGGAAAAAGAAGAATGACCCTTCGTTTCTGGAAAAAAAGGAGCGTTGCGATTACC TCAAGAACAAACTGTCCCACATAAAGCAAAGAATTCAAGAATACGATAAAGTGATGAATTGGGATACGCA AGGTTATCCCTAGACACTGTTTAGCCGCTGCTGCTGTGTGCTGGTCTCCTGGGTGAGCAGCAGGCACCTC CAGGTCATCTTCCGCTTGGTTGAAGCACATCCTAGGCAAACGCACTGTGACTTCACTCAGACATCTCGAA ACCGTTTCAAACCCGCGAGGGCCTTTACTGAGATGAGGGGGACAGCGTATTCAGCTGGGATAGTTTAAGG TTCTTCTGATGAACAAGGGCTTGAAGCAAGATCAGTTGTCACACTCTGTGCTCTTGGGCGAGTTGGTCAG TCCTCTGTGCCTGTTTCTTTTGTAAAATGATACCTACCTCATAGGGCAGTTATGAGACCATATGTGCTCA CCTTTATTTCTCAAGCATCTGGAACAGAGCCTCCTGCTGTTGTTGCTGCTGCTGCTATGGAAGAGCCACG TTCGTGTGGGGAGGGCTGTGGGCCCGTTTTTGGAGGATGAGCTACACAGGATGAGTCTCAGGAAGCAGTC CATAGTGAGCAAGGGTCAGCAGCTAACCAGAGGCGCGTGTCCCATAATGCCCTGGCGTTTTTCCGCCTTT AGCACTGTAGCCTTAAGTGGAAGTTTCACTGCTGCCTGTGAACTGTTATGGCCCCTCACGGCCTCCATGC CTGTAATCAGGGTCACTGTGCATAGATCCCCGTGTCCCACAGCCCACGCTGTGTGCATGTGAGAGCTAAG CCTTTCAGTTTACTTGGTGTCTCCCCTGTGATTCATTGCCACACCTACCCCATCTGTGTGACAGTTTCCA CCCACTACAGCTTCCAGTCAAACAATTAACCAATCAATGTCCGAAGGGTTTTACAAAGTAGCGAAACCAA AGTAATTAATGAGTGGAGCTGGCAGAGGCCTGGAAAGTTCTGGGTGCTGATGACAAAGTTTTGTTTTGTT TTGTTTTGTTTGTCAGTGAAACCTGGCAATACTCTTGCAGACTAACTTTTGTAGTGAGACCTTGTCTCAG AGCACGAAACAAATAAAAACAAAATAAAGGAGGAAAAAGACAAGTAGTAAATAAATAAAACATGGTAGCA CGTGCACACCACCCTGACACTCAAGAGCAGAGGCAGGAGTGTGTCGAGTTCAAAGCCAGCCTGGGCTTCA GAGCAAGGACCCCCTCTCTCTTTAATGCAAGATATTGCCGTTTTGTACATACATTTAAAAAGCTTAGGTT AGCGTTAAATTTAAACGTTAAACACACTTAACTTTGTGAGACTTTATTTTACTACAGTTGTAAATGTTAA TCTTTTAAGACTGATGCTGTAACACTGATTTATTTTTTAATGTTCTATCAATACAGAGGTGTATTAAAAT GTCAGTTACCTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5066185 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5066185 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11048 same embryo
	EMAGE:11047 same embryo
	EMAGE:11043 same embryo
	EMAGE:11046 same embryo
	EMAGE:11044 same embryo
	EurExpress:euxassay_001109 same experiment
	MGI:4826106 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source