EMAGE mouse gene expression spatial database

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EMAGE:11074

Cdc14b CDC14 cell division cycle 14 homolog B (S. cerevisiae) ( MGI:2441808)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003505_01	euxassay_003505_02	euxassay_003505_03	euxassay_003505_04	euxassay_003505_05

euxassay_003505_06	euxassay_003505_07	euxassay_003505_08	euxassay_003505_09	euxassay_003505_10

euxassay_003505_11	euxassay_003505_12	euxassay_003505_13	euxassay_003505_14	euxassay_003505_15

euxassay_003505_16	euxassay_003505_17	euxassay_003505_18	euxassay_003505_19	euxassay_003505_20

euxassay_003505_21	euxassay_003505_22	euxassay_003505_23	euxassay_003505_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11074_wholemount_strong.wlz

11074_wholemount_moderate.wlz

11074_wholemount_weak.wlz

11074_wholemount_possible.wlz

11074_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11074_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7923
Entity Detected:	Cdc14b, CDC14 cell division cycle 14 homolog B (S. cerevisiae) ( MGI:2441808)
Sequence:	sense strand is shown >T7923 CCGCGGCCCGCTGAGCTCGGACGCCACGGCAGCAGCGTCCCAGGGAGCGCGCGCTTTGCTCCAGCCACCG GCCAGCGCTGTGGGGCCCCCGGGTCGCGGGCGGCGGGAGACGCAGCGCGAGGACCCGGGCCGGGTTGCCG GGCCATGGTCGCGGTCCCCTGACGGGCCGCGACCGCCTCCATGAAGCGTAAGAGCGAGCGGCGCTCGGCT TGGGCCACCGCGCCCCCCTGCTCACGCCGCAGCTCGTCCTCCTCTCCCGGCGTGAAGAAGAGCCGCAGCT CCACGCCGCAGGAGCTGCACCGCCTGGAGCAGCAGGACGACCTGTACCTGGACATCACGGATCGCCTTTG TTTTGCCATTCTCTACAGCAGACCAAAGAGTGCAACAAACGAACATTATTTCAGCATAGATAACGAGCTA GAGTATGAGAACTTCTATGCGGATTTTGGACCACTCAATCTGGCCATGGTTTACAGATATTGTTGCAAGA TAAATAAGAAGCTCAAGTCCATTACAATGCTGAGGAAGAAAATTATCCATTTTACTGGCACTGATCAGAG AAAGCAAGCGAATGCCGCCTTCCTTATCGGGTGTTATATGGTTATATATTTGGGGAGGACACCAGAAGAC GCGTATAGAACATTAATCTTCGGAGATACAGCCTATATTCCTTTCAGAGATGCTGCCTATGGAAGCTGCA GTTTCTATATTACACTTCTCGACTGTTTTCACGCAGTAAAGAAGGCAATGCAGTATGGCTTCTTTAATTT CAACTCATTTAACCTTGACGAATATGAACACTATGAAAAAGCAGAAAATGGAGATTTCAACTGGATAATA CCAGAGCGATTTCTTGCCTTCTGTGGGCCCCATTCAAGATCCAGGCTGGAAAGTGGTTACCACCAACATT CTCCGGAGACTTATATTCCATATTTTAAGAATCACAATGTAACTACCATTATTCGTCTGAATAAAAGGAT GTATGATGCTAAGCGCTTTACGGATGCTGGATTCGATCACCATGATCTTTTCTTTCCGGATGGCAGCACC CCTGCTGAGTCAATTGTTCAAGAATTTCTGGATATTTGTGAAAATGTCAAGGGTGCCATTGCCGTGCATT GCAAAGCTGGCCTTGGTCGAACAGGCACTCTGATAGGCTGCTACCTCATGAAGCATTACAGGATGACAGC AGCTGAGAGCATTGCCTGGCTCAGAATCTGTAGACCCGGCTCAGTAATTGGGCCCCAGCAACAGTTTCTG GTCATGAAACAATCAAGCCTCTGGCTAGAAGGCGACTATTTCCGTCAAAAGTTAAGAGGGCAGGAGAATG GCCCCCTCAGAGAAGCCTTCTCCAAACACCTTTCGGATGCTGATGACCTGTCCTTAAATGGGCTTGAGAA TCAAGACAATCAAGAGCCTGAGCCGTATAGTGATGATGATGAAGTCAGTGGAATGACACAAGGAGACAGA CTTCGGGCCCTGAAAAGCCGGAGACAGCCGAAAGCCAGCGCTATCCCCCTCACATGTCCCCTAGCTGTGC TGACCTCTGCACTGTGTAGTGTTGCCATCTGGTGGATTGTGTGTGACTACATTCTTCCCACCCTGCTGTT CTGTCTTGATGGCTTCAGAACACAGTAGCCATCTGGACCCTTTGACAGATAGTTTGCTGTTCCAGCCATT TCAAGGACTAAGACAGTCTTGCGTTAAAGTAAAAAAACGGTGACCAGCACTCCAGGGAAGACTAGGAACA GAAAACCCAAAAAGGATTTAGCCAATCAATCTGAGAACAAAGCAGACTCTGCAATGGCCTTAGCTTCTTA CCACATAAGAAGTTCATTCAATGAAGAAAATGTCACTGGCCTCTCATCCACTCACTGACTCTGGGGTGTG GTTGAACCATTTGAGGGCCTGACTCTCCCTTTTGTTATCTCAAGTTGGACAAAAGCTGCTGTTGTGAACT TAGTCTCAGGTGTCAATATCCTGTCCTCTGCCACCAGAGAGTTGTCTGGTGAGTGTGGTACATGTGTGTG TGTCCCTGTGATGGCAATCATGTTTGCTGCTGGCCTCCAGAAGAGTTGAGGATCTGATGGAGATATCTTA GGTATTTATTTTTTGTTCAACCTTGTGACCCTGACTCGCAGTTTATAAAGATGAAAGAGATGAACATCCC ACCAGTGGTACCCTCCATCTTCAGGTATGATTTGGTACAATCAGCTTCACTTTCCTCTTGTTAATCCTGT TGTGATGTTCACTGTGTGAAGTACCCAAGCTGCTTGTTTTTCACCAAAGAGTGCTTTATTAACAACCTGT GAACACTGCATTTAAACACTGTTGTGTGTTGTTGTGGTTACTGAAAACCTGCTAGGAAATGTTGAGAATG TTGGAACCCTTTCTCAGTTACCTGGTTCCTACAAATCCTTCCTTGTGTCATTTAGGATGAAGATTTCAGA GCTGGGATGTTGTTGGGTTGCTCAGTCACGTTATAATTGTGTTTACTTCATTACATCAAAACCAAACATC AACCCTAGCATTTTCCTTTGGGAAAGCATCCTAAATAATGGTGGCTTTAACAACAAAATTGTGAGCCTTG CTGTATTGGCGCACGCCTTGAATCCCAGCATTGGGAGGCAGAGGCAGGTGGATCTCCAGGTGGAGTTCCA GGCCAGCCAATGATACACAAGGAAATCCTGTCTCAAAAATAAAACAAAGCAAAACAAAAAAACCAAACCC AAAATCCCAAAATTGTTAACCCTTTGTAGAGTCTTTCTCTGTAACGCTGTGGAGTTTTTTCCTTGTGATC CACAGTTTGATTTTAGTGACAATCACTTGCTTCTGGAGTTAGGCTGTTTGTGTAAGAGATCTCACTGTTG CCTTTGGTTAGGGAATATGACTAGAAACTGTAGTGTGCCTTTTCTCTTCACCCTGCTGGAAGCCAGCAGC ACCCGTGCCATGAAGCAATTGCAAAACCAGAATTTATCTGCTGCCACAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6514098 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6514098 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11075 same embryo
	EMAGE:11073 same embryo
	EMAGE:11076 same embryo
	EurExpress:euxassay_003505 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source