EMAGE mouse gene expression spatial database

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EMAGE:11075

Eif2s3x eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked ( MGI:1349431)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003509. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003509_01	euxassay_003509_02	euxassay_003509_03	euxassay_003509_04

euxassay_003509_05	euxassay_003509_06	euxassay_003509_07	euxassay_003509_08	euxassay_003509_09

euxassay_003509_10	euxassay_003509_11	euxassay_003509_12	euxassay_003509_13	euxassay_003509_14

euxassay_003509_15	euxassay_003509_16	euxassay_003509_17	euxassay_003509_18	euxassay_003509_19

euxassay_003509_20	euxassay_003509_21	euxassay_003509_22	euxassay_003509_23	euxassay_003509_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11075_wholemount_strong.wlz

11075_wholemount_moderate.wlz

11075_wholemount_weak.wlz

11075_wholemount_possible.wlz

11075_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11075_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 04 24

foot mesenchyme

strong

regional

strong expression: see section 03 04 05 20 21

hip mesenchyme

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23 24

knee mesenchyme

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23 24

lower leg mesenchyme

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23 24

upper leg mesenchyme

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23 24

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16

tail mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7915
Entity Detected:	Eif2s3x, eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked ( MGI:1349431)
Sequence:	sense strand is shown >T7915 CTTTTCTCTCCGAGCAAGATGGCTGGGGGTGAGGGTGGAGTGACTCTCGGTCAGCCCCATCTTTCCCGAC AGGATCTCGCCACATTGGATGTTACCAAGTTGACACCACTTTCACATGAAGTTATCAGCAGACAAGCTAC AATTAATATTGGTACAATTGGTCACGTAGCTCATGGGAAGTCCACTGTGGTGAAGGCTATTTCTGGAGTT CACACTGTCAGATTCAAAAATGAACTCGAAAGGAACATTACCATCAAGCTTGGATATGCTAATGCTAAGA TTTATAAACTTGATGACCCAAGTTGTCCTAGACCAGAATGTTACAGATCGTGTGGAAGCAGCACACCAGA TGAGTTTCCTACAGACATTCCGGGGACCAAAGGGAACTTCAAGTTAGTCAGGCATGTTTCCTTTGTTGAC TGTCCTGGCCATGATATTTTGATGGCTACGATGCTGAACGGTGCAGCAGTGATGGATGCGGCCCTTCTGT TGATAGCTGGTAACGAATCATGCCCTCAGCCCCAGACTTCCGAACACCTGGCTGCCATAGAAATCATGAA ACTCAAGCATATTTTGATCTTACAGAATAAAATTGATCTGGTAAAAGAAAGTCAGGCTAAGGAACAATAT GAGCAAATCCTCGCATTTGTGCAAGGAACCGTAGCAGAAGGGGCACCAATTATTCCAATTTCTGCTCAAC TGAAGTACAATATTGAAGTTGTCTGTGAGTATATAGTGAAGAAAATACCAGTGCCCCCAAGAGACTTTAC TTCAGAGCCCCGTCTCATTGTCATCAGGTCATTTGATGTCAACAAACCTGGCTGTGAAGTCGATGACCTT AAGGGAGGTGTAGCTGGTGGTAGTATCCTAAAAGGAGTGTTAAAGGTAGGCCAGGAGATAGAAGTGAGAC CCGGTATTGTCTCCAAAGACAGTGAAGGAAAACTCATGTGTAAACCAATCTTTTCCAAAATTGTGTCACT TTTTGCGGAACATAATGATCTTCAATATGCTGCCCCGGGTGGTCTCATTGGAGTTGGAACAAAGATCGAC CCCACCTTGTGCCGAGCTGACAGAATGGTGGGACAAGTGCTCGGTGCAGTTGGAGCTTTACCTGAAATAT TCACCGAACTGGAGATTTCCTATTTCCTGCTTAGACGTCTTCTGGGTGTACGTACTGAAGGAGACAAGAA AGCAGCAAAGGTCCAAAAGCTGTCTAAGAATGAAGTGCTTATGGTCAACATAGGCTCCCTGTCGACAGGA GGAAGAGTTAGTGCAGTCAAGGCTGATTTGGGCAAAATTGTTTTGACCAATCCCGTGTGCACAGAAGTAG GAGAAAAAATTGCCCTTAGCAGAAGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGTCAGATAAGAAG AGGAGTGACCATTAAGCCAACTGTAGATGATGACTGAAGACTTCAAGTTAAACAATGCCTTGGGAAGGAG TTGGAATTTCTTTTACTAGCCTAGGGGTATATTTTCAAAGCAATACTAGAAAGAATTAATTTCCTAGCTC ATTACCTTACTAGTAGCAGCTATAAATTTATTCTTATATTTTGGCGATGAAAATGTGACCTCTACTTTTT TTTTTCTTTTTATTGATTCCTTGTTTGACATTATGTAGCCCAATCCCATTCATCCCCTCATATCCAACCT TTGCCCATGCGACCTCCCCCAAAGTAGCACACCAACAACAGAACAAAGCCTAGAAAACATGCCATCGTGG AAGCTGTAGCTTCTGTGACAACAACAATGTTGGCTCCTCATCAGGACTCCCTCCAGTTATCCTGTTGCTG CGCTGTGTCATAGAGATCCTGCAGCTTTGGAACAGCAGGGTGGCCCTTTCACGATCCCCACCATTCGAAC ATGACATAGATTTTAGGTGGGTCAATTCAGAGCCCCGGATAAGGGCCAGGATGGTAGCTGAGCTGGTCAG CTGGGGCCCATTCTTCCAAGTGCTGCGGCTTGTGAGAGCCTGGGCCAATTCTCCCTACAGAAGGTGGCAA CGGGGGAGGGTGTCCCATCCACACCACCTCATAACAGACAAGTGGCAGGGCCAGCTGTCCAGAGTGCTAT ATCCAATGAGGGATGGTGGCAGACCTGCACAGCCTCAGACACCCAGTGTTCCCTAGTGGTAATAATGAGC CATGGACACTGACCCACCACTGTGGATCCACAGACTCAAACAAGGCCCTGATGGCAGGGATGCCCACTTA TAACAGGCTACTTCTCTCCACCCTTGAATTCCTTTTTCTCTCCCGTCTGTCATGGTGACAAGCAGGTATC TATGCTTATGCACTGGAGGGCAGGACTGTGGGTGGCATGGAGGTCTACAAGACCTTGTGTTCCTCTTCTC ATGCCGTGCTGCCTTGCTTTAATTTGATTTTTATGAGTCCTAGGCATAAGACAAGGTTTGGCCATCAAGC TAGGATGAACAAAGGACTGCCATCTGCTCTGCCCCTGATAAAGAACAATACCACCAATAAGGGGTCTCTG CTGGTTGCTGGGGTTGTAACCTACTTCTAATATGGGGCTTAAAATTAAATGTGAAAAGTGGTATACTATA TACATTGGATCAAGTCTATATAGTAGCTAAAATTAAGCATTCCAAAGTAATGTCTAATTTATCAATCAAT GAAAGTTTGAAGTGAAACTTGGCTGCAACCAGTCTTTGGCGTAGCACTTGTATCATGAACCTGTCTAAGA TGAAAATGATCTCTTCTCATGATTCATCTGTGAGTAGCTCCATAGTGAAGGGCAATTAGTAAAGGCTAAA GACTTACCCCTTTATTTGTCCTTCTATTTGGGGTTCTGTTGTCTGAAATTTGAAGGAATACGACCAGTGG GCTCAAATGAGCACCACTCTTAGAAGTTGTGTGCCCATTCCCTTAACACAGGTAGATCTGTCTTACCCGT TTCCTGGGTGGGACCTTAGGAATCCAGTGCCCTGAGACTATGTCTTTATTATTTATGCCTAGTATCTCAG TCTTCCCACACTGGCAAAGAGTATAGCAACTGGTGGTTAGTGATAATTGACAAGGTTCTGTCCAGGCTGC TTTTTTGAGGTCTTTGTTTTAGTAAGCTATGAATGGAGCTGTCTCTTGCATGATGAATAAACACTCTACC ATTGTCTCCACTGCCCCTAGCTGTAGTACTGGCTGATAGGATTATGGTGGAAATTGTGTTATTGGTAATC TTTGAGCCTTGAGAGAGGTAGAAATTGTTCAGAAACTGTGTCCTCTGGTTTCTAAATCTATTCCCCACGT GCCTCCAATTTTCTTGTGCTGTGCATTTTAAATTAATCATGTTTTTTCTTAATTAAATACTCTTTCACAG CAATGATTTCCAACTCTGGCTGACGTAAGAATTGCTGGGGAAGTTTGAAGGGGGGAAAAAGAAAGCTCTG GGGTGGAGTCCAGGCTTTGATAGTTTTTTTTGTTTGTTTGTTTGTTTGTTTTTTTTTTTTGAGGAAACCT ATTGATATATGTATTTGATTGACTCTAAAAAGTTGTTGTATTGGAACATGAAGAAAACATTTAAATAAAA CAAGATTCTGAAACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6513251 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6513251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11074 same embryo
	EMAGE:11073 same embryo
	EMAGE:11076 same embryo
	EurExpress:euxassay_003509 same experiment
	MGI:4824506 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source