EMAGE mouse gene expression spatial database

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EMAGE:11101

Ctsb cathepsin B ( MGI:88561)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003561_01	euxassay_003561_02	euxassay_003561_03	euxassay_003561_04	euxassay_003561_05

euxassay_003561_06	euxassay_003561_07	euxassay_003561_08	euxassay_003561_09	euxassay_003561_10

euxassay_003561_11	euxassay_003561_12	euxassay_003561_13	euxassay_003561_14	euxassay_003561_15

euxassay_003561_16	euxassay_003561_17	euxassay_003561_18	euxassay_003561_19	euxassay_003561_20

euxassay_003561_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11101_wholemount_strong.wlz

11101_wholemount_moderate.wlz

11101_wholemount_weak.wlz

11101_wholemount_possible.wlz

11101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6249
Entity Detected:	Ctsb, cathepsin B ( MGI:88561)
Sequence:	sense strand is shown >T6249 CGGAGCTGCGCGGGTACTTAGGAGTGCACGGGAGAGGCCAGGAGCGGCTCCGTTTGGCTTGGTGGCTGCA GGCCCAGGCTGTCGCGCTGCGCTCGGTGAGTGCAGGATCCAGCATCCAGGATGTGGTGGTCCTTGATCCT TCTTTCTTGCCTGCTGGCACTGACCAGTGCCCATGACAAGCCTTCCTTCCACCCGCTGTCGGATGACCTG ATTAACTATATCAACAAACAGAATACAACATGGCAGGCTGGACGCAACTTCTACAATGTTGACATAAGCT ATCTGAAGAAGCTGTGTGGCACTGTCCTGGGTGGACCCAAACTGCCAGGAAGGGTTGCGTTCGGTGAGGA CATAGATCTACCTGAAACCTTTGATGCACGGGAACAATGGTCCAACTGCCCGACCATTGGACAGATTAGA GACCAGGGCTCCTGCGGCTCTTGTTGGGCATTTGGGGCAGTGGAAGCCATTTCTGACCGAACCTGCATTC ACACCAATGGCCGAGTCAACGTGGAGGTGTCTGCTGAAGACCTGCTTACTTGCTGTGGTATCCAGTGTGG GGACGGCTGTAATGGTGGCTATCCCTCTGGAGCATGGAGCTTCTGGACAAAAAAAGGCCTGGTTTCAGGT GGAGTCTACAATTCTCATGTAGGCTGCTTACCATACACCATCCCTCCCTGCGAGCACCATGTCAATGGCT CCCGTCCCCCATGCACTGGAGAAGGAGATACTCCCAGGTGCAACAAGAGCTGTGAAGCTGGCTACTCCCC ATCCTACAAAGAGGATAAGCACTTTGGGTACACTTCCTACAGCGTGTCTAACAGTGTGAAGGAGATCATG GCAGAAATCTACAAAAATGGCCCAGTGGAGGGTGCCTTCACTGTGTTTTCTGACTTCTTGACTTACAAAT CAGGAGTATACAAGCATGAAGCCGGTGATATGATGGGTGGCCACGCCATCCGCATCCTGGGCTGGGGAGT AGAGAATGGAGTTCCCTACTGGCTGGCAGCCAACTCTTGGAACCTTGACTGGGGTGATAATGGCTTCTTT AAAATCCTCAGAGGAGAGAACCACTGTGGCATTGAATCAGAAATTGTGGCTGGAATCCCACGCACTGACC AGTACTGGGGAAGATTCTAATCTGCTTTGACTTCATTGTCCAGTCCTTAGGGGCTTTTTCCAAAATTTAG CGGCCTTGGCAGGGAATGAGGTAGACAGGGGAGTCTTTGATTCTTTGAGTTCATCTAACATGCATGAAGC TTTTGGCAAGATTTGGACGACTGGACCAGAGCTGCCTGCCATCAGAGCTACCCTCCAAACACTGTCCTTA CCTGGCCTCCTCCTAGCCCATCCCACGACAGTGATCACAGTCTGCATCCTAGCCTCTCCCTAGACTAGTG CGGTTTGTAGTGCCTGCTGTTGTGGCTCTGCCTGCCATACCTCCCACCCCCATCCCCATCCCAGCATCCG AGGACCCAAGGGCTTTGACTGCAGGACTTCCAAAAGAACGAACACTTCACGAGAGGACAAATGCCACCTC TCAGCTGCATCTTGAAGCTGGTCACTTCTGGGTCTGTGGCAGGCATTCGTCAATGGCCCAGTGCAGTTCT CTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCATCGACATTGGTAATGTGGCCAGCTCTCCTTGG TCCTGTCCTCAGCCGATGCTTTTTCAATAGGATTTTTATGCTTTGTGTACCTCAACCAAGTATGAAGAGC TGTACTGGTTTTATAGATCACCCCCAATTGTATGGCTTAAAACAAGCTACATGGTCATGGTTTGCTGTGT GACCGACAGATAACCACAATGAAACTAGTCTGGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3500700 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500700 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11104 same embryo
	EMAGE:11103 same embryo
	EMAGE:11099 same embryo
	EMAGE:11100 same embryo
	EMAGE:11102 same embryo
	EurExpress:euxassay_003561 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source