EMAGE mouse gene expression spatial database

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EMAGE:11102

Ubac1 ubiquitin associated domain containing 1 ( MGI:1920995)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003575. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003575_01	euxassay_003575_02	euxassay_003575_03	euxassay_003575_04

euxassay_003575_05	euxassay_003575_06	euxassay_003575_07	euxassay_003575_08	euxassay_003575_09

euxassay_003575_10	euxassay_003575_11	euxassay_003575_12	euxassay_003575_13	euxassay_003575_14

euxassay_003575_15	euxassay_003575_16	euxassay_003575_17	euxassay_003575_18	euxassay_003575_19

euxassay_003575_20	euxassay_003575_21	euxassay_003575_22	euxassay_003575_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11102_wholemount_strong.wlz

11102_wholemount_moderate.wlz

11102_wholemount_weak.wlz

11102_wholemount_possible.wlz

11102_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11102_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 19 20 weak expression: see section 21

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19

dorsal root ganglion

strong

regional

strong expression: see section 18 moderate expression: see section 08 09 10 11 12 17

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6716
Entity Detected:	Ubac1, ubiquitin associated domain containing 1 ( MGI:1920995)
Sequence:	sense strand is shown >T6716 GCAAGGGTACAGCTCCTGCCCCTTCAGTGACGGGCGGCGGAGCTGACAGGAGCCGGGGCGGGGCGGCGGC GGCCAGCGAAGGAGCGCACGGCGGCCCGGCCCCGCCCCTGGTCCTCCCGCCAGCCTCAGTGACCTTCACT GGTTCGGGCGGCGGGTGCAGGCCCGGGCGGCGGCGCGATGTTCGTGCAGGAGGAGAAGATCTTCGCGGGC AAGGTGCTTCGGCTGCACATCTGCGCAGCCGACGGCGCCGAGTGGCTGGAGGAGGCGACCGAGGACACAT CGGTGGAGAAGCTCAAGGAGAGTTGCCTCAAACATGGTGCTCATGGAAGCTTAGAAGACCCCAAGAATGT GACCCACCACAAGCTAATACATGCTGCTTCAGAGAGGGTGCTGAGTGACAGCAAAACGATCTTGGAAGAA AACATCCAGGACCAAGACGTCTTGTTATTGATAAAAAAGCGTGTTCCTTCTCCACTCCCTAAGATGGCTG ACGTCTCAGCAGAAGAAAAGAAGAAACAAGAGCAAAAGGCTCCAGACAAAGATGCTATTCTTCGAGCAAC GGCAAACCTGCCTGCCTGCAGCACTGACCGGACTGCTGTCCAGACCACCATGAGAGACTTCCAGACAGAA CTCCGGAAGATCCTCGTGTCTCTCATTGAGGTGGCACAGAAGCTGTTAGCACTGAATCCCGATGCAGTTG AATTGTTTAAGAAGGCAAATGCTATGCTGGATGAAGATGAGGATGAGCGAGTGGATGAAACTGCCTTGCG GCAGCTCACAGAGATGGGCTTTCCTGAGAGCAGAGCCTCAAAGGCCCTTCGGCTGAACCACATGTCAGTG CCTCAGGCCATGGAGTGGCTAATTGAACACTCAGAAGATCCAGCTATTGACACACCTCTTCCAGGCCATG CTGCTCAAGCAGGGGCCAGTGGTGCAGCCACTACCTCCTCCACCTCCTCTGAAGCTGCTGTGGGAACTAG TGCTGAAGATGAGGAGACCCGGGATGAGCTCACAGAAATCTTCAAGAAGATACGCAGGAAAAAGGAATTT CGAGCAGATGCTCGGGCTGTCATTTCGCTGATGGAGATGGGCTTTGACGAGAAGGAAGTGATTGATGCCC TCCGAGTGAACAACAACCAGCAGAACGCTGCTTGTGAATGGCTGCTGGGGGACCGGAAGCCATCCCCTGA GGAATTGGACCAGGGCATCGATCCCAATAGCCCTCTCTTTCAGGCCATCCTGGACAACCCAGTGGTGCAG CTGGGTTTGACAAACCCTAAGACATTGCTAGCATTTGAAGACATGCTGGAGAATCCGCTTAACAGCACCC AGTGGATGAACGACCCAGAGACGGGCCCGGTCATGCTGCAGATCTCTAGAATCTTCCAGACGCTGAATCG CACGTAGGTGGTACCTACTGTGCTCGTTGGCTGTGCAGTCTCAGCAGTCTGTGAGGCAGTGGGCACAGGG TTCAGCCGGGATCTTGCCTGGACCACAGCTTCTCGGACTCTCCTTTCCAGACCTAACTGCTTATCAACTT CTGCCACATGGTTTGAAAGTTGCAGTCCTAGCTTCTGACAGGTGTCTACAGAAAGTGGCTGTTGCATTGG GAAGGTGAATGATTGCTGCCCGTCCCTCCAGATACTCTGCTGGGCTCCTGAAGTGCTACTTGAATTGACC CTTAGGTGTTTGCTTGTGGAGCCCAATGTATTTAAAAGAAGGCATGTTCTGATCCAAAGTGTTATGTCAA GTGTGCTAATTTAACCAGACTGACTCACAGATGGCTTCTTTAATAAATAATCCCTTTTCCTAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3662474 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3662474 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11104 same embryo
	EMAGE:11101 same embryo
	EMAGE:11103 same embryo
	EMAGE:11099 same embryo
	EMAGE:11100 same embryo
	EurExpress:euxassay_003575 same experiment
	MGI:4829047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source