EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11103

Tgfb3 transforming growth factor, beta 3 ( MGI:98727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003567. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003567_01	euxassay_003567_02	euxassay_003567_03	euxassay_003567_04

euxassay_003567_05	euxassay_003567_06	euxassay_003567_07	euxassay_003567_08	euxassay_003567_09

euxassay_003567_10	euxassay_003567_11	euxassay_003567_12	euxassay_003567_13	euxassay_003567_14

euxassay_003567_15	euxassay_003567_16	euxassay_003567_17	euxassay_003567_18	euxassay_003567_19

euxassay_003567_20	euxassay_003567_21	euxassay_003567_22	euxassay_003567_23	euxassay_003567_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11103_wholemount_strong.wlz

11103_wholemount_moderate.wlz

11103_wholemount_weak.wlz

11103_wholemount_possible.wlz

11103_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11103_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6717
Entity Detected:	Tgfb3, transforming growth factor, beta 3 ( MGI:98727)
Sequence:	sense strand is shown >T6717 CGATATCAGATATCCGCTCTATTTATTTTTACCTAAGGAAAAACTCCAGCTCTCTTCCCACTCCCAGCTG CCTTGCCACCCCTCCCAACCCTCGGCTTGCCCTCCACCTGGCCTGCTGGGAGTCAGAGCCCGGCAGAACC TGTTTAGACATATGGACAAGGATCCTAGCTCTACCCAGCACACGGTCCGGTCCCGCTACTTCGTCCGGGG GTTGCCAGCGCTTCCTGGGAGTCCTGAAGCTCACCCAGCGCAGTGAGTTCATGCACCCTCTTGCCAAGCC TCAGGCTTTGGGATCTGGGGAGGACGCCTGGTTTTCCTCCCCCTTTCTACTGGGGTCTCCTCCTTGCCAG GCCTCCCCAGTCCCCTGGCCTCGTTCCTGGGCTCACACATGAAGATGCACTTGCAAAGGGCTCTGGTAGT CCTGGCCCTGCTGAACTTGGCCACAATCAGCCTCTCTCTGTCCACTTGCACCACGTTGGACTTCGGCCAC ATCAAGAAGAAGAGGGTGGAAGCCATTAGGGGACAGATCTTGAGCAAGCTCAGGCTCACCAGCCCCCCTG AGCCATCGGTGATGACCCACGTCCCCTATCAGGTCCTGGCACTTTACAACAGCACCCGGGAGTTGCTGGA AGAGATGCACGGGGAGAGGGAGGAAGGCTGCACTCAGGAGACCTCGGAGTCTGAGTACTATGCCAAAGAG ATCCATAAATTCGACATGATCCAGGGGCTGGCGGAGCACAATGAACTGGCCGTTTGCCCCAAAGGAATTA CCTCTAAGGTTTTTCGTTTCAATGTGTCCTCAGTGGAGAAAAATGGAACCAATCTGTTCCGGGCAGAGTT CCGGGTCTTGCGGGTGCCCAACCCCAGCTCCAAGCGCACAGAACAGAGAATTGAGCTCTTCCAGATACTT CGACCGGATGAGCACATAGCCAAGCAGCGCTACATAGGTGGCAAGAATCTGCCCACAAGGGGCACCGCTG AGTGGCTGTCTTTCGATGTCACTGACACTGTGCGCGAGTGGCTGTTGAGGAGAGAGTCCAACTTGGGTCT GGAAATCAGCATCCACTGTCCATGTCACACCTTTCAGCCCAATGGAGACATACTGGAAAATGTTCATGAG GTGATGGAAATCAAATTCAAAGGAGTGGACAATGAAGATGACCATGGCCGTGGAGACCTGGGGCGTCTCA AGAAGCAGAAGGATCACCACAACCCACACCTGATCCTCATGATGATCCCCCCACACCGACTGGACAGCCC AGGCCAGGGTAGTCAGAGGAAGAAGAGGGCCCTGGACACCAATTACTGCTTCCGCAACCTGGAGGAGAAC TGCTGTGTACGCCCCCTTTATATTGACTTCCGGCAGGATCTAGGCTGGAAATGGGTCCACGAACCTAAGG GTTACTATGCCAACTTCTGCTCAGGCCCTTGCCCATACCTCCGCAGCGCAGACACAACCCATAGCACGGT GCTTGGACTATACAACACCCTGAACCCAGAGGCGTCTGCCTCGCCATGCTGCGTCCCCCAGGACCTGGAG CCCCTGACCATCTTGTACTATGTGGGCAGAACCCCCAAGGTGGAGCAGCTGTCCAACATGGTGGTGAAGT CGTGTAAGTGCAGCTGAGGCCAGCCTGCCACAGAGGAGGGAAAACTGCCACCACAGACTGCTGCTCCTTG GAAAACCAAAGCGACAGACCTCACCCTAGAGGCCTGGAGCCCAGAAGCCCTGGCTCCCGGCGAAAGGCCG AGAGGGAGGCTTCCTTCTGGAACATTTCTTTCTGCTAGCTTTGAGAATCACTGTGGTAAAGAAAGTGTGG GTTGGTTAGGGGAAGGCACACTGTTGAGAACATGGAATTTCTGTGGTGCAGGCAGAGGTGGTGGGGTAGA GAACGGATAGCGAGTGGACATTGTGAGTGGCAATGGGCCTCGGGCTACCCTACAAAAGATGAAGGGTAGA GTGGTCAGTCAGGTGAAGGCCAGACTGGAAGGCATTTCAGATCTGAGGTCAGCTTTGCTCACTGCAGTAC ATCTGCTCTAGGGAATTGGGATGTCGTTAGAGGCGGCCAAGCATTTTTCTTCAGTTTACCAAGCCAAAGT CCCCTGGAATCATATCTTGCACTGCCTGGAATTAAGGACAATCCGTTCTTTCTGCAACTGTCTTTTCACC TCGATCAGCATCATGAGTCATCACAGATCATGTAATTAGTTTTTGGGCCAGCAACTAGCTATCTCAGGTC CCTTAGAGATGCTGGACTCAAAAGCAGAGGTCAGAATTGGTTCTCTCATGTATTCCCTGGGGGCCCCTCC TTCTGCCTTCCTTTTTGGCCGCATTTCCCCTTGGATATTTGGCTAGACACCTTCCGGGTCAGGGTGCATT TTCCGGATTCAGAGTCCACATAGCCTGGGGCGCCATTTCCCTCCTACCCTAGACCCTGTGCTCCCCAGGT GTTCCTGGAAGCAGGTGCTACATGCCAAGTATCCGGGGAGCCTCCCATGTTTGACATGATGACTCGGCCC CAGATGCACAGACTGAAGTATAAAGACAAATACAAATATTACTTTCAGAATCTTTGTATAAATAAATATT TTTGGGGAATCATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3663150 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3663150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11104 same embryo
	EMAGE:11101 same embryo
	EMAGE:11099 same embryo
	EMAGE:11100 same embryo
	EMAGE:11102 same embryo
	EurExpress:euxassay_003567 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source