EMAGE mouse gene expression spatial database

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EMAGE:11104

Arl8a ADP-ribosylation factor-like 8A ( MGI:1915974)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003560. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003560_01	euxassay_003560_02	euxassay_003560_03	euxassay_003560_04

euxassay_003560_05	euxassay_003560_06	euxassay_003560_07	euxassay_003560_08	euxassay_003560_09

euxassay_003560_10	euxassay_003560_11	euxassay_003560_12	euxassay_003560_13	euxassay_003560_14

euxassay_003560_15	euxassay_003560_16	euxassay_003560_17	euxassay_003560_18	euxassay_003560_19

euxassay_003560_20	euxassay_003560_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11104_wholemount_strong.wlz

11104_wholemount_moderate.wlz

11104_wholemount_weak.wlz

11104_wholemount_possible.wlz

11104_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11104_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 03 04 05 weak expression: see section 06 07 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 16 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 03 04 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 05 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 02 03 04 05 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 08 14 15

cervical ganglion

strong

regional

strong expression: see section 06 14 15

thoracic ganglion

strong

regional

strong expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 13 14 15 16

not examined

regional

not examined expression: see section 01 20 21

neural retina

strong

regional

strong expression: see section 01 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 04 05 06 07 08 09 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6709
Entity Detected:	Arl8a, ADP-ribosylation factor-like 8A ( MGI:1915974)
Sequence:	sense strand is shown >T6709 GCGTCGTCGGCCGCCGCGCTCCAGCCTCCGCCCGCACCGAGCCGCGGGACCCGGCCGCACCGGGGAGGGG CCGCTCCGGGCCGCAGCGCGAGGGCACCGAAGGCGGCGGGGACCCGGCACCGGGCGCGGCCGGTGGCAGC GACCATGATCGCTTTGTTCAACAAGCTGCTGGACTGGTTCAAGGCCCTGTTCTGGAAGGAGGAAATGGAG CTCACGCTGGTGGGGCTGCAGTACTCGGGCAAGACCACCTTCGTCAACGTGATCGCGTCAGGACAGTTCA ATGAGGACATGATCCCCACTGTGGGTTTCAACATGCGAAAAATCACCAAAGGGAATGTGACCATCAAGCT CTGGGACATTGGGGGGCAGCCCCGTTTCCGAAGCATGTGGGAACGCTACTGCAGAGGAGTGAGCGCCATT GTGTATATGGTGGATGCTGCGGATCAGGAGAAGATCGAGGCCTCCAAGAATGAGCTCCACAACCTGCTAG ACAAGCCACAGCTACAAGGCATCCCGGTCTTAGTCCTTGGTAACAAGCGAGACCTCGCCGGAGCGCTAGA TGAGAAGGAGCTAATCGAGAAAATGAACCTGTCTGCTATCCAGGACCGAGAGATCTGCTGCTACTCCATA TCCTGCAAAGAGAAGGACAACATAGACATCACCCTACAGTGGCTTATTCAACACTCAAAGTCACGGAGAA GCTGAGACTGTGGCTCTTCTTCTGACTAGGGACCACAGTCACCTAACCTGAAGCCGAGCTCCCCGCCCAC CCTGTTGTCTCTCCCTAAGCCCACTCCTCCTCACCCAGTGTGGGGAGGGCCCTCTGGGGCTCCTAGAGCC CTATTCTGCTGAGGTTTCAACTCCTGTTTTTATTGTAAAATAATTGCCCTCTCCCCAAGTCTGGTCCCCT AACCTCTCTCCCTTGCTCTCTTTTTCCCTTCAGCTACCCCACTCCCCTCCCTGTCAACAGCCCTGGCCCC GCAAGCCCTCTCCTCACTCTCTGCCCCACCCTCCTCTTCCCCTTTCAACACTCTGTTATTGTCCTGTGTG TACAGTATATATATGTATATATATTTTAATTTTTTAATTTAAGCAAAGACTAAGATCAACCATTTGATGC TGCAGGGGCCAATCGGGACCTGGGAGGGGCAAACTGGAAAGGCAGAGAGAGATGCAGGGTCGATGTGGGG CAAGTTCATATGCAGAAGAGGGGAGACTGCCACATAGGGCAGTGGTTGTCCCCATGTCCTGTAAAGTATC TCTGTCTCCCAGTGGAGGACCTGAATTTGAAGGCCAGCAGGAGCCTCCTTTTCTGTCCCCAGCCTTGATA GGGCCACCAGGTACAACCAGGTCTAAGTGGGAGGACCAGGGTCCATGTCGTCCCAGCAACCCCCACTGTC ACTGTTCCTGGCCCCAGGTCCTGCCCCTACCCAGGGTCCCCACCTCCACGGCCCATCTCATTTTCTGTTC TCATTTCACAGAGTTGTACAAGGAGAGAACTCAGCATGGGAGGGTTGGTTCTTTGGTTTTGTTTGTTTGT TTGTTTGTTTGTTTAATTTAATAATTTGTAAAGTGATGTTCCTCTTCCTTTTTACAGTTTTCAGCTCATA TTTAACCTCTGTTTGGAAAACGATCCTTGTAACTGTACATTTTTTTTTTTTGCCTCCTAATAATGACAAC AATAAATTACCAATTTTGTGTTGAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3661329 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3661329 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11101 same embryo
	EMAGE:11103 same embryo
	EMAGE:11099 same embryo
	EMAGE:11100 same embryo
	EMAGE:11102 same embryo
	EurExpress:euxassay_003560 same experiment
	MGI:4823244 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source