EMAGE mouse gene expression spatial database

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EMAGE:11164

Dgkz diacylglycerol kinase zeta ( MGI:1278339)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003474. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003474_01	euxassay_003474_02	euxassay_003474_03	euxassay_003474_04

euxassay_003474_05	euxassay_003474_06	euxassay_003474_07	euxassay_003474_08	euxassay_003474_09

euxassay_003474_10	euxassay_003474_11	euxassay_003474_12	euxassay_003474_13	euxassay_003474_14

euxassay_003474_15	euxassay_003474_16	euxassay_003474_17	euxassay_003474_18	euxassay_003474_19

euxassay_003474_20	euxassay_003474_21	euxassay_003474_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11164_wholemount_strong.wlz

11164_wholemount_moderate.wlz

11164_wholemount_weak.wlz

11164_wholemount_possible.wlz

11164_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11164_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 16 17 18

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 19 20 21 22

telencephalon

moderate

regional

moderate expression: see section 01 02 05 06 07 08 09 10 14 15 18 19 20 weak expression: see section 03 04 11 12 13 17 21 22

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 14 15

pons mantle layer

moderate

regional

moderate expression: see section 06

facial vii ganglion

strong

regional

strong expression: see section 04 06 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 22 weak expression: see section 21

midgut

strong

regional

strong expression: see section 12 13 moderate expression: see section 08 09 10 11 14 15 16 17 18

not examined

regional

not examined expression: see section 08 09 10 11 12 13 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6727
Entity Detected:	Dgkz, diacylglycerol kinase zeta ( MGI:1278339)
Sequence:	sense strand is shown >T6727 GGGGCGCGAGGACGCGCAGCGCAGCGCAGGGCGGGCGGAGCGCGCGCTCGCCATGAGCTTGGCGGGCGGT GCGGAGCCGGCGTGCTGAGCCCCGGCCGCCGGCCCGGCATGGGCGTCTCCCGCGGGCCCGCCGCCGGCCG GGGCTAGGGCCGGATGGAGCCGCGGGACCCCAGCCCCGAGGGCCGGAGCAGCGACTCGGAGTCGGCCTCC GCCTCGTCCAGCGGCTCCGAGCGCGACGCGGGTCCGGAGCCCGACAAGGCGCCGCGGCGCCTGACCAAGC GGCGCTTCCCGGGGCTGCGGCTCTTTGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCAGCACCTGGC ACCCCCTCCTCCCACGCCCGGGGCCCCGTGCGGTGAATCTGAGGAGCAGATCCAGAGCACTGTGGACTGG AGTGAGTCAGCAGTGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGTGACTTCTGCTATGTCG GGGAGCAGCACTGTGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAAAAAGTGTGCAGCCTGTAAGAT CGTGGTGCATACCCAATGCATTAAGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCGTCCTTCCGCGAA TCAGGCTCCAGGAATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGACGACGCCAGGATG GCAAGTGTCGGCACTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGATCGTAGCCAT CAGCTGCTCCTGGTGCAAACAGGCATACCACAGCAAGGTGTCGTGCTTCATGATGCAACAGATTGAGGAA CCCTGCTCCCTAGGGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTGCGGGCCCGGAGGCCCC AGAACACCCTCAAGGCCAGCAAGAAGAAAAAGAGAGCGTCCTTCAAGAGGAGGTCCAGCAAGAAAGGACC TGAGGAAGGCCGCTGGAGACCCTTCATCATCAGACCCACCCCATCCCCCCTCATGAAACCCCTGCTGGTG TTTGTGAACCCCAAGAGTGGGGGCAACCAGGGTGCCAAGATCATCCAGTCTTTTTTGTGGTATCTGAATC CCCGACAAGTCTTTGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAAAGTACATAA TTTGAGGATTCTGGCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTCTCCACCCTGGACCAGCTGCGC TTAAAACCACCGCCTCCTGTAGCCATCCTGCCTCTGGGCACTGGCAATGACCTGGCCCGAACCCTCAACT GGGGTGGGGGTTACACAGATGAGCCTGTGTCAAAGATCCTTTCCCATGTTGAGGAGGGGAATGTGGTACA GCTGGACCGTTGGGACCTCCGAGCAGAGCCCAACCCTGAGGCGGGGCCTGAGGAGCGAGATGATGGAGCC ACTGACCGGCTGCCCCTGGATGTCTTTAACAACTACTTCAGCCTGGGCTTCGATGCCCACGTCACCCTGG AATTTCATGAGTCTCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGATGTTCTATGC TGGGACGGCCTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGAGTAGTGTGT GACGGAATGGACCTAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATCGTCTTTCTGAATATCCCCA GGTACTGTGCAGGCACCATGCCCTGGGGCCACCCCGGGGAGCACCATGACTTCGAGCCCCAGAGGCATGA TGATGGCTACCTGGAGGTCATCGGCTTCACCATGACATCCTTGGCAGCACTGCAGGTGGGTGGGCACGGC GAGCGATTGACGCAGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGGTGGACGGTG AGCCCTGCAAGCTTTCAGCATCACGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGTGCAGAAGGC CAAGCGCCGGAGTACTGCCCCACTGCACAGCGACCAGCAGCCAGTTCCTGAGCAGCTGAGGATCCAGGTG AGCAGGGTCAGTATGCACGACTACGAGGCTCTACATTATGACAAGGAGCAGCTCAAGGAAGCCTCTGTGC CTCTGGGCACTGTGGTGGTCCCCGGAGACAGTGACCTGGAGCTCTGCCGCGCCCACATTGAGAGACTGCA GCGGGAGCCCGATGGGGCTGGAGCCAAGTCCCCGATGTGCCACCAACTCTCCTCCAAGTGGTGTTTCCTG GATGCCACCACTGCCAGCCGCTTCTACAGGATCGACAGAGCCCAGGAGCACCTCAACTATGTGACGGAGA TTGCCCAGGACGAGATTTACATCCTAGACCCAGAGCTTCTGGGAGCATCAGCCCGGCCTGACCTCCCCAC CCCTACCTCCCCACTCCCTGCTTCCCCCTGCTCCCCTACACCCGGGTCAATGCAGGGGGACACTGCACTG CCCCAAGGTGAAGAACTGATTGAAGCAGCCAAGAGGAATGACTGCTGCAAGCTCCAGGAGCTACACCGAG CAGGAGGTGACCTCATGCACCGTGACCAGAAGAGCCGCACGCTCTTGCACCACGCGGTCAGCACTGGCAG TAAGGAAGTGGTCCGCTATCTGCTGGATCATGCACCTCCAGAGATCCTTGATGCTGTGGAGGAGAATGGG GAGACCTGTCTACACCAGGCAGCCGCCCTGGGTCAGCGCACCATCTGCCACTACATTGTGGAAGCCGGGG CCTCCCTCATGAAGACAGATCTGCAGGGCGACACTCCCCGGCAGCGCGCTGAGAAGGCTCAAGACACAGA GCTCTCTGCCTACCTGGAGAACAGACAGCATTACCAGATGATCCAGCGTGAGGACCAGGAGACAGCTGTG TAGTTAAGGGGACCATGGATCAACAAGGGACACTACCAGAGGACTCTCCTTGCCCATCTCACTGCCACAT TCCTGTCAGATGGCTATGGGGGGACCCTGCCACAGGGAAGGAGCCCCGTGCCACCCCCTGAGAAGCTGTT CAGATCTAGGGCTGGACTCTAAGGAGCTGGACTCTCACCTGTCCCTGGTTTCATGGGGAACAGGAAACAG GCTGGGCTGGCTGGTTCCCTCCCTTCAGGGCAGCCTCCAGCTCACCACAGCAGATGGAAGGGCTGGACAG AGCTCAGCATCGATGTCAGGGAGGCCTGCACTCAGCAGGACTTTCAAAAGCCACCTGATCCTTTGGGCTC TCTGGAGGGTTCTGGGTACCTAACCCTCCTCTCTGATCCACCCACCCTGGGCATCCCAGAAACTCAAGAG CCTGCTGTATTCACTTGCCCGCTGCCCTGCTTGGCACCTACCCTGGTCTTCCTCCTCATGTATCCAGCCA TTTCATTTCGGACTGTATGGCCTGGGGTGGGGGGTGGGGCTCCCACGGTGACTTGTTTACAGCTGGGTGT GACTCAGTAAAGTGAATTTTTTTTCCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3668704 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3668704 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11169 same embryo
	EMAGE:11166 same embryo
	EMAGE:11168 same embryo
	EMAGE:11165 same embryo
	EMAGE:11167 same embryo
	EurExpress:euxassay_003474 same experiment
	MGI:4824294 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source