EMAGE mouse gene expression spatial database

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EMAGE:11222

Zw10 ZW10 homolog (Drosophila), centromere/kinetochore protein ( MGI:1349478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003422. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003422_01	euxassay_003422_02	euxassay_003422_03	euxassay_003422_04

euxassay_003422_05	euxassay_003422_06	euxassay_003422_07	euxassay_003422_08	euxassay_003422_09

euxassay_003422_10	euxassay_003422_11	euxassay_003422_12	euxassay_003422_13	euxassay_003422_14

euxassay_003422_15	euxassay_003422_16	euxassay_003422_17	euxassay_003422_18	euxassay_003422_19

euxassay_003422_20	euxassay_003422_21	euxassay_003422_22	euxassay_003422_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11222_wholemount_strong.wlz

11222_wholemount_moderate.wlz

11222_wholemount_weak.wlz

11222_wholemount_possible.wlz

11222_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11222_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6821
Entity Detected:	Zw10, ZW10 homolog (Drosophila), centromere/kinetochore protein ( MGI:1349478)
Sequence:	sense strand is shown >T6821 CGCTGGCAGCGGGGTTGACCTATCCTGTTTGTGTGGGTACGCTTCGTGGCGATGGCCTCTTTCGTGACAG AAGTCTTGGCCCACTCGGGGAGCCTGGAGAAGGAGGATCTGGGCACCAGGATCAGCCGCCTGACCCGGCG GGTGGAGGAGATCAAGGGTGAAGTATGCAATATGATCAGCAAGAAGTACAGTGAGTTCCTGCCTACCATG CAGAGTGCGCAGGCCCTAGTTACCCAGGTGGACACTCTATCTAACGACATCGACCAGCTGAAATCCAGGA TAGAGACTGAGGTCTGTCGGGATCTTCACATATCAACTGTTGAGTTTACAAACCTGAAGCAGCAGCTGGA AAGAGACTCTGTAGTCCTAACTCTGCTCAAACAACTCCAAGAGTTTTCCTCTGCTATTGAAGAGTATAAC AGTGCGTTAGCAGAGAAGAAGTACATCCCTGCTGCTCGGCACCTGGAGGAGGCTCAGGAGTGCTTGAAGT TATTAAAGTCCAGAAAATGCTTTGACTTAAAAATGTTGAAGTCTCTCAGCATGGAGCTCACCGTGCAGAA GCAGAACATACTGTACCACCTGGGAGAAGACTGGCAGAAGCTGGTTGTATGGAAGTTTCCGCCGGCAAAA GATACCAGCAGCTTAGAGTCTTGTCTACAAACAGAGCTTCATTTATGCACTGAGCAGCCGGAGAAAGAGG ACATGACTCCTCTGCCGTCCATCAGTTCTGTCCTCTTGGCCTTTTCCATTCTTGGAGAATTACCCACCAA GCTGAAGTCATTTGGTCAGATGCTGCTGAAGTATATCCTTAAGCCTCTGGTGACCTGCCCGTCGCTCCAT GCTGTGATTGAAAGGCAGCCAAGTTCAGTTAGCATTTGTTTCGAGTCTCTGACGACGGACTTGGAACACC CATCACCACCCGAAGCTTTTGCAAAGATCCGATTGGTCCTGGAAGTGCTCCAGAAACAGCTTCTAGATTT GCCTCTTGATGCTGACCTAGAAATTGGAAAAGTCCCTGGGATCGTGTTGGCTGAGATGCTTGGTGAGGGG ATCTGGGAGGACCTTTCTGAGTGCCTCATCAGAAACTGCTTGGTTTACTCTATTCCCACAAACAGCAGCA AGCTCCAGGAATACGAAGAGATTATACAGTCCACAGAAGAATTTGAAAAGTTCCTAAAGGAGATGAGGTT TTTAAAAGGCGATACTACTGACTTGCTGAAATATGCTCGCAATATCAATTCTCATTTTGCTAATAAGAAG TGCCAGGATGTGATTGTGGCGGCTAGAAATCTCATGACCTCTGAGATTCACAACACTGTCAAGATTGGTC CCGATTGTAAGGAAGCCTTACCAGACTTACCCAGTCCTGATGCTGATCACAAGCTACAAGTACAGACGGT CTGCAAAGCGCAGTTCACCGACGCCGGGAACCTAGAGCCTGAGACTTCTCTGGACCCTCAGTCCTTTTCT TTACCCACGTGCCGCATCAGCGAGGCTGTGAAGAAGTTAATGGAGCTCGCTTATCAGACTTTACTTGAGG CAACAACAAGTAGCGACCAATGTGCTGTCCAGCTCTTCTACTCAGTGAGGAATATCTTCCATTTGTTCCA TGATGTGGTACCAACATACCACAAGGAGAACCTTCGGAAGCTCCCCCAGTTGGCTGCCATTCACCACAAC AACTGCATGTATATTGCTCACCACTTGCTGACCCTCGGTCATCAGTTCAGATTGCGGCTCGCCCCCATCC TTTGCGATGGCACGACCACCTTTGTGGATCTTGTACCTGGCTTCAGAAGACTTGGGACGGAGTGCTTTTT GGCCCAAATGCAGGCACAGAAAGGAGAACTTCTGGAGAGATTGTCAAGTGCTAGAAGCTTTGCGAACATG GACGACGAAGAGAATTACTCTGCAGCGAGCAAGGCAGTGAGGCAGGTGCTGCACCAGCTAAGGAGACTTG GGATCGTGTGGCAGGATGTCCTGCCAGTGAACATATACTGCAAGGCCATGGGGACCTTGCTGAACACAGC GATTGCTGAGATGATGAGCAGGATCACTGCTCTAGAGGACATCTCGACTGAAGATGGTGATAGATTGTAT TCCCTGTGCAAAACAGTGATGGATGAAGGTCCACAAGTGTTTGCACCTCTGTCTGATGAAAACAAAAACA AGAAATACCAAGAAGAGGTTCCCGTCTATGTGTCCAAATGGATGCCTTTCAAAGAACTCATGATAATGCT ACAAGCTAGCTTGCAAGAAATTGGAGATCGGTGGGCAGATGGAAAAGGGCCCCTGGCAACTGCATTCCCT TCCAGCGAAGTCAAAGCTTTAATCCGTGCCTTGTTCCAGAACACAGAAAGAAGAGCAGCTGCCCTTGCTA AGATTAAATAGCTCCTCCTTGGAGAAAGGCTCTCCTGACTCTGGGTTCCTCCTTTGGTGTAATTATTTCC TTCAAAACCTCTCGGAATTACTTGTTGGTTTTGGTGAGCCAGTATATCATGGAAGCGTAACTTCACATAA GGACATCCTTGCCTGGACGAAGGTTTTCTGAACTGGACATTACGATGATCAATTTACGCAAAGCACTGGT GGGTTCATTGACTGTCGCTGCTGTCCCTTGTCAAGTATGAGTCTGCTCCGCAGTGTGATTTGTTCCCAGG CCCTCTTGAGAGTTGTATACATTATAATTTTAGACCCTGGCTTTGGACATTCCAAAGTTAGGGTTCAGAA TTTGTAAAATACGTTTTTTGAGGGGGGTGGGCATTAAGGGCACATTACTTGTTCTTACAAGAATTACAGA AACCTCTTAAAATATTTCTTTCCAGTAAATGCCTTTCAGAGTTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3711269 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711269 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11223 same embryo
	EMAGE:11220 same embryo
	EMAGE:11219 same embryo
	EMAGE:11221 same embryo
	EurExpress:euxassay_003422 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source