EMAGE mouse gene expression spatial database

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EMAGE:11223

Abcb6 ATP-binding cassette, sub-family B (MDR/TAP), member 6 ( MGI:1921354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003398. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003398_01	euxassay_003398_02	euxassay_003398_03	euxassay_003398_04

euxassay_003398_05	euxassay_003398_06	euxassay_003398_07	euxassay_003398_08	euxassay_003398_09

euxassay_003398_10	euxassay_003398_11	euxassay_003398_12	euxassay_003398_13	euxassay_003398_14

euxassay_003398_15	euxassay_003398_16	euxassay_003398_17	euxassay_003398_18	euxassay_003398_19

euxassay_003398_20	euxassay_003398_21	euxassay_003398_22	euxassay_003398_23	euxassay_003398_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11223_wholemount_strong.wlz

11223_wholemount_moderate.wlz

11223_wholemount_weak.wlz

11223_wholemount_possible.wlz

11223_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11223_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

ventral grey horn

weak

regional

weak expression: see section 11 12 13 14 15 16 17

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6814
Entity Detected:	Abcb6, ATP-binding cassette, sub-family B (MDR/TAP), member 6 ( MGI:1921354)
Sequence:	sense strand is shown >T6814 CGTGAAGAGAGTGGGCAGTGTAGCAGGTCCACGTGCGTCCCTTCCCGCGACCCAAGCGGCGTAGCTCCAT GTCCCAGGACACCCTGGCATCTGCGGCCCTCTCTGAAGGGTCAGCCCGACTCTTTGTGCAGTGACCTCAA GTTCAGCCAGCAGCCACACATCGCCATGGTGACTGTGGGCAACTACTGCGAGACCGAAGGGCCGGCGGGG CCCGCTTGGACTCAGAATGGCTTGAGTCCCTGCTTCTTCTTCACGCTAGTGCCCTCGACACTCCTGACTC TGGGGGTACTGGCCTTGGTGCTGGTTCTTCCCCGCAGGCGTCGGGAGGTGCCTGCTGGCCCAGAAGAGCT GTCTTGGGCGGCTGGCCCTCGGGTGGCCCCATATGTGCTGCAGCTGTTTCTGGCCACACTTCAGATGGCA CTGCCCCTGGCTGGTCTGGCTGGCCGAGTGGGCACTGCTCGGGGGGTCCGTCTGCCAGGCTACCTACTGC TGGCTTCTGTGCTGGAGAGTCTGGCCAGCGTCTGTGGCTTGTGGCTGCTCGTGGTGGAACGGAGCCAGGC ACGGCAGAGTCTGGCAATGGGCGTCTGGATGAAGTTCAGGCATAGCTTGGGTCTCCTGCTCCTCTGGACT GTGACATTTGCAGCCGAGAACTTGGCCCTAGTATCTTGGAACAGCCCGCAGTGGTGGTGGGCCAGAGCAG ATCTGGGCCAGCAGGTTCAATTTGGCCTGTGGGTCCTGCGTTATGTGACCTCTGGAGGGCTATTTATCTT GGGGCTCTGGGCCCCAGGACTTCGCCCCCAGTCTTATACTCTACACGTTCATGAAGAAGACCAAGATGTA GGAGGGAACCAGGGCCGCTCAACAGACCGACGATCCACCTGGAGAGACCTTGGCCGGAAGCTTCGACTGC TGAGTAGCTACTTGTGGCCTCGGGGAAGTCCATCACTACAGCTGATAGTGCTCATATGCCTGGGACTCAT GGGGTTGGAACGAGCACTCAATGTGTTGGTCCCCATCTTCTATAGGGACATTGTGAACTTACTGACTGCG AAGGCTCCTTGGAGCTCCCTGGCCTGGACCGTTACCACCTATGTCTTCCTCAAGTTCCTCCAGGGGGGTG GCACTGGCAGTACAGGTTTTGTGAGCAACCTGCGAACCTTCCTATGGATCCGGGTGCAGCAGTTTACGTC CCGAGGTGTGGAGCTACGTCTCTTCTCTCACCTGCATGAACTCTCACTGCGCTGGCACCTGGGCCGGCGT ACCGGGGAGGTGCTTCGGATCGTGGACCGGGGAACATCTAGTGTCACAGGACTACTCAGCTACCTGGTGT TCAGCATCATCCCCACCCTGGCTGACATCATCATTGGCATCATCTACTTCAGCATGTTCTTCAATGCCTG GTTTGGCCTCATCGTGTTCCTGTGCATGAGTCTTTACCTCATCCTGACTATCGTGGTCACCGAGTGGAGA GCCAAGTTTCGCCGTGATATGAACACACAGGAAAACGCCACCCGGGCACGAGCAGTAGACTCTCTGCTCA ACTTTGAAACGGTGAAATACTATGGTGCCGAGGGTTATGAAGTAGACCGCTATCGAGAGGCCATCCTCAA GTTTCAGGGTTTGGAGTGGAAGTCAACCGCCTCACTGGTTGTGCTGAATCAGACACAGAACCTGGTGATC GGGCTTGGCCTCCTTGCTGGCTCCTTGCTTTGCGCATACTTCGTCAGTGAGCAGAAGCTACAGGTTGGGG ACTTTGTGCTTTTTGGCACTTACATCACCCAACTGTACATGCCTCTCAACTGGTTTGGCACCTACTACAG GATGATCCAGACTAACTTCATTGACATGGAGAACATGTTTGACTTGCTGAAAGAGGAGACAGAAGTGAAG GATGTTCCTGGTGCAGGGCCCCTTCGTTTTCATAAGGGCCGGATTGAGTTTGAAAATGTGCACTTCAGCT ACGCTGATGGGCAGGAGACCTTACAAGATGTGTCCTTCACTGTGATGCCTGGACAGACGGTGGCTCTGGT AGGTCCATCTGGGGCAGGAAAGAGCACAATTTTGCGCCTGCTATTTCGCTTCTATGACATCAGCTCTGGC TGCATCCGAATAGATGGACAGGATATTTCACAGGTGACCCAGATCTCTCTCCGATCTCACATTGGAGTTG TGCCCCAGGACACTGTCCTCTTCAACGACACCATTGCTAACAATATCCGCTATGGCCGTGTCACAGCTGG GGACAGTGAGGTAGAGGCTGCTGCTCAGGCTGCAGGCATTCATGATGCCATCTTGTCTTTCCCTGAAGGG TATGAGACACAGGTCGGGGAGCGGGGTCTGAAGCTGAGTGGTGGCGAGAAGCAGCGAGTGGCCATCGCCC GCACCATCCTTAAGGCTCCTGACATCATTCTGCTGGATGAGGCCACATCAGCACTGGATACATCTAATGA GAGAGCCATCCAGGCCTCTCTGGCCAAAGTCTGCACCAACCGCACCACCATCGTCATAGCACACAGGCTC TCAACTGTGGTCAATGCTGACCAGATTCTGGTCATCAAGGACGGTTGCATCATAGAGAGAGGAAGGCACG AAGCCCTGCTGTCCCGAGGTGGCGTTTATGCCGAGATGTGGCAGCTGCAGCAGCAAGGACAAGAAACTGT CCCTGAAGAATCTAAACCTCAGGACACGGCATAGTAATAAAAGGGACCTCTTTCCTTCCAAAGGCGAACT CAGGAGAAAAAGATATGTCCCCTCTCTTGCCTTCATTTCATCCTGGTGTAGGAGTTTGGTGCTAGCTATT GTAAGGAAAAGGGACCTTCCAGAAAGCATCGTGAGGAAATAAATGGCCTGCGCAAGGACGGCTGTGGTTT GGTTGTGTGGGGTACTGCAAGGGGTCTCTCCCCTTGAGCTGAATGAACATTCCCGAAAAAGAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710552 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710552 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11220 same embryo
	EMAGE:11219 same embryo
	EMAGE:11222 same embryo
	EMAGE:11221 same embryo
	EurExpress:euxassay_003398 same experiment
	MGI:4822862 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source