EMAGE mouse gene expression spatial database

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EMAGE:11228

Peo1 progressive external ophthalmoplegia 1 (human) ( MGI:2137410)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003198. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003198_01	euxassay_003198_02	euxassay_003198_03	euxassay_003198_04

euxassay_003198_05	euxassay_003198_06	euxassay_003198_07	euxassay_003198_08	euxassay_003198_09

euxassay_003198_10	euxassay_003198_11	euxassay_003198_12	euxassay_003198_13	euxassay_003198_14

euxassay_003198_15	euxassay_003198_16	euxassay_003198_17	euxassay_003198_18	euxassay_003198_19

euxassay_003198_20	euxassay_003198_21	euxassay_003198_22	euxassay_003198_23	euxassay_003198_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11228_wholemount_strong.wlz

11228_wholemount_moderate.wlz

11228_wholemount_weak.wlz

11228_wholemount_possible.wlz

11228_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11228_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4892
Entity Detected:	Peo1, progressive external ophthalmoplegia 1 (human) ( MGI:2137410)
Sequence:	sense strand is shown >T4892 GGAGAGTGGTGACGCGAAGCTGGGGGCGGGAGCGTGGAGCTTTGAAGTGGAGGGCAGAGCAAGGGAGCCA GTGAGGAAGCCGGGCCGGAGCGCGTGACCTCCAGCAGTGTGGAGAGTGCCTGTGAGCGCTGGGGGCTCGG AGCGCCTGGAGTTGGGGTTCAGACGGGATGCAGAAAGCGGTAGAGATGAGGTTAGCCAGGAGAGTCCGTA GAGGGGTAGTAGACGTGAAGAGATGATACAGAGAAGCCGTTTAGATCGAGGGACCGTAGAAGGATCTAGA AGTGAGCTGGTGGAGAGAAATCCGTAAAGGACCTAGAGATAGGAGAATCCTCAGAGAGAGGCGTAGACGC GAAAGGGCAGTGTAGGTGTGTAGGGATGAAGTAGCAATACACAGGCAACCGAGGGGAGTAAGAACTGTGT TCGTGAAGAAGTTTGAGGAGGGATAAAGAGAAAAGCCAGTAGGAGTGATTTCTGCGAGTGCCTTGTAGAA GGTTTCCTAGGGTCTGGTTTAGTGAAGGCACTGAACCAGACAACAAAAGCTTTTGCAGTAGTGGCGTCCC ACTCCATTAGGAATGTGGCTTCTTCTCCGACGTGCATATCCCCTCCGGATCTTGCTGCCACTGCGTGGCG AGTGGGTGGGTAGGAGGGGCCTGCCCAGAAGCTTGGCGCCAGGCCCTCCCCGCAGAAGGTACAGAAAAGA AGCGCTCCCAGCCTTAGAGATGCCAGTGTCGCCGGTGACTACGACTGAAATCCGCCAGTATTTACGTGCA CATGGGATCCCCTTCCAGGATGGCCACAGCTGCCTTCGAGCACCAAGTCCCTTTGTGGTGTCCTCAGACA TCAAAAATGAGAAAAAGGACGCTCCCACTTCCTTCTGCCTCTTCATTGACAAGACCACAGGACACTTTCT TTGCATGACCAGCCTAGCAGAAGGGAGCTGGGAAGACTTGCAGGCCAGTGTGGAGGGACGAGGGGATGGG GCCAAAGAGGGTGTCCTACTTAGGGAAGGCCCAGAAGCTGAGGTTAGGGAGGAGGTCTTGAGGATCTGGA ACCGAGCCATACCTCTTTGGGAGCTTCCAGACCCTGAGGAAGCCCAGCTAGCTCGTGTGATGTTTGGCCT TACCAAGGTGACAGATGACACACTCAGGCGATTCAGTGTCCGCTATCTTCGATCTGCTCGAAGTCTGGTC TTCCCTTGGTTCACCCCTGGGAGCTCAGGATTACGTGGCCTGAAGCTACTAGGGGCTGAGGGTCAGGAAA ATGGAGTACAGTATGTAGAGACCACCATCCCCCGGCCTGGTGTCTATCACAACCTATTTGGATTACCACT GATCAGCCGGAGAGATACTGAGGTGGTTGTGACAAGTCGTGAACTGGACAGCCTGGCCTTGAGCCAGTCG ACGGGGCTGCCAACCCTTTCCCTACCCCGAGGGACAGTCTGCTTACCCCCAGCCTTACTGCCTTACCTTG AACAGTTCCGTCGGATTGTGTTCTGGTTAGGAGATGACCTTCGATCCTGGGAAGCTGCCAAACTGTTTGC TCGAAAACTCAACCCCAAGCGATGCTCTCTGGTGAGGCCTGGGAACCAACAGCCTCGTCCCCTGGAGGCC TTAAACCAAGGCTTAAGTCTTCCCCGTATTCTTCGTACCGCCCTACCTGCCTGGCACAAGTCTATTGTGT CTTTTCGGCAGCTCCGAGAGGAGGTTTTAGGAGAACTGTCAAATGTAGAGCAAGCGGCTGGTGTCCGTTG GAGCCGCTTCCCAGACCTGAATCGTCTGCTGAAGGGACATCGGAAGGGCGAGTTGACGGTCTTTACAGGG CCAACAGGCAGTGGCAAGACAACATTCATCAGTGAGTATGCCCTGGACTTGTGTACCCAGGGAGTGAACA CACTGTGGGGTAGCTTTGAGATCAGCAACGTGAGACTAGCCCGGGTCATGCTGACCCAGTTTGCTGTGAC ACGGCTGGAAGAGCAACTGGACAAGTATGAAGAGTGGGCAGACCGTTTTGAGGACCTGCCTCTCTACTTC ATGACTTTCCACGGACAACAGAGCATCAGGTCCGTCATAGACACAATGCAACATGCTGTCTACGTCTATG ACGTCTGTCATGTAGTCATTGACAACTTGCAGTTCATGATGGGTCACGAGCAGCTCTCCTCTGACAGGAT TGCAGCTCAAGACTACATTGTTGGAGCCTTTCGGAAGTTTGCTACAGACAACAGTTGCCACGTGACTCTG GTCATTCACCCTCGGAAAGAAGACGACGACAAGGAACTGCAGACAGCATCCATTTTCGGCTCAGCCAAAG CAAGCCAAGAAGCAGACAATGTTCTGATCTTACAGGATAGGAAACTGGTGACTGGGCCTGGGAAACGGTA TCTGCAGGTGTCCAAGAATCGCTTTGATGGAGACGTAGGTGTCTTCCCACTGGAGTTCAACAAGAATTCC CTTACCTTCTCCATCCCACCCAAGAGCAAAGCCCGACTCAAGAAGATCAAGGACGACAATGGACTAGTGG CCAAAAAATCCTCTTCTGGCAAAAAAGGGGCTGCGCACCAGAACCCTGAGATTTGCTTAGGCCAGGACCC CAGCCCCGCCCAGCCAGATACCTCCAAGTCCTCAGGGTGAAAGCAGTGCACTGCTGCTCGCTGAAATGAG CCTGGTGGAGCAGGCCTTCTGCTGGGGCTCTTCCATATAGTCCTGAGCTACAGAACCCTTCCCAGCCCAG GGCTGGACTCCATGGTGAAAATTTTTAATTGAGCAGCTACTGGGGAATCTCCTGTATGCTGGTTTGTGTT TGTTTTTTCCCCATAGGTTTTCTGTATAGCACTGGAAAGACAAAGGCTGTTTCCAAAGAACCAGCGTTTT GGTGAAAGGAACGAAGCAAAGGGAAGGATGCTAGACTCAGCTCGTTTTGGGTTTTTGTTTTTTGTTTTTC CAGATAGGGTTTCTTCTCTGTATAGCCCTGGCTGTCTTGGAACTCACTTTGTAGACCAGGCTGGCCTCGA ACTCAGAAATCCGCCTGCCTCGTCAGCTTGTTTTGATTAGTCAGTCTGAAAAGGTGTCTGAACAGAGTGC ATGGGAGAACTAGACATGGGCCTGTGAACCTCTGCAGAAAGGGATCCAGATGGAAACACGCCAGACCCTC AGGTGGGAATGAGCAAGGCAGATGAAGAGGCCAGGCCCCTGAAGCTGCCGTGAAACAGGAGGTTTGCTGA AGCTCCAGAGCCGAAGGCTGATGCGGTAGGCACCGAGCCTCTCTGCTCTGCTTTGTAGAGCCAGGCAGAA GAATTGATCTGAGGTGCTGATCCTGTACTCTTAGGGCTTCAAAGATGGAAGCTGCTTCCCACTGGACCAT GGAAAGAAGCTGTAAGCCTGCAGTGCCTGCCCCGTGGGTGCCGATTTCACTGCTGCCTCTGACAGCTGGA GCTAGGACTCTTTTGTAGAGCCTTTTCCAGTTTGAATGAAACATTTCTATTGCCTTTGAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5100486 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100486 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11233 same embryo
	EMAGE:11232 same embryo
	EMAGE:11230 same embryo
	EMAGE:11229 same embryo
	EMAGE:11231 same embryo
	EurExpress:euxassay_003198 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source