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EMAGE:11232

Bhmt betaine-homocysteine methyltransferase ( MGI:1339972)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232
"Pseudo-wholemount" of euxassay_001266. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001266_01 euxassay_001266_02 euxassay_001266_03 euxassay_001266_04
EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232
euxassay_001266_05 euxassay_001266_06 euxassay_001266_07 euxassay_001266_08 euxassay_001266_09
EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232
euxassay_001266_10 euxassay_001266_11 euxassay_001266_12 euxassay_001266_13 euxassay_001266_14
EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232
euxassay_001266_15 euxassay_001266_16 euxassay_001266_17 euxassay_001266_18 euxassay_001266_19
EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232 EMAGE:11232
euxassay_001266_20 euxassay_001266_21 euxassay_001266_22 euxassay_001266_23 euxassay_001266_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11232Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11232_wholemount_strong.wlz
11232_wholemount_moderate.wlz
11232_wholemount_weak.wlz
11232_wholemount_possible.wlz
11232_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11232_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4894
Entity Detected:Bhmt, betaine-homocysteine methyltransferase ( MGI:1339972)
Sequence:sense strand is shown

>T4894
CCACGCGTCCGCAGCTACCCTCCAATCCGCAGCTGTGTTCAGCGCCACTCACTACACCGGAAAGATGGCA
CCAGTTGCTGGCAAGAAGGCCAAGAAGGGGATCTTAGAACGCTTAAATGCCGGAGAAGTTGTGATTGGAG
ATGGAGGATTTGTCTTTGCACTGGAAAAGAGGGGCTATGTAAAGGCTGGACCCTGGACCCCAGAAGCTGC
CGTGGAGCATCCTGAGGCAGTTCGTCAGCTTCATCGGGAGTTCCTCAGAGCTGGATCGAACGTCATGCAG
ACCTTCACTTTCTATGCAAGTGAAGACAAGCTGGAAAACAGAGGAAACTATGTGGCAGAGAAGATTTCTG
GGCAGAAAGTCAACGAAGCTGCTTGTGACATTGCACGGCAAGTGGCTGATGAAGGAGACGCTTTGGTTGC
AGGAGGCGTGAGCCAGACGCCTTCATACCTTAGCTGCAAGAGTGAGGTAGAAGTGAAAAAGATATTTCGC
CAACAGCTAGAGGTGTTCATGAAGAAGAACGTGGACTTCCTCATTGCAGAGTATTTTGAACATGTTGAAG
AAGCCGTGTGGGCAGTGGAAGCCTTAAAAGCATCTGGTAAGCCCGTAGCAGCTACCATGTGCATTGGGCC
CGAGGGAGATCTGCATGGCGTGCCCCCTGGAGAGTGTGCCGTGCGTCTGGTGAAAGCAGGCGCCTCCATT
GTCGGCGTGAACTGCCACTTCGACCCCAGCGTCAGCTTACAGACTGTGAAGCTCATGAAGGAGGGTTTGG
AGGCTGCGCGGTTGAAAGCTTACCTGATGAGCCAGCCCCTGGCCTACCATACCCCTGACTGTGGCAAACA
GGGATTTATTGATCTCCCAGAATTCCCCTTTGGATTGGAACCCCGAGTTGCCACTAGATGGGATATTCAA
AAATATGCCAGAGAGGCCTACAACCTGGGGGTTAGGTACATTGGCGGCTGCTGCGGATTTGAGCCCTACC
ACATCAGGGCGATTGCAGAGGAGTTGGCCCCAGAAAGGGGATTTTTGCCACCGGCTTCAGAAAAACATGG
CAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGATCAGAGCAAGGGCCAGAAAGGAATACTGG
CAGAATCTGCGAATAGCTTCCGGCAGGCCGTACAACCCTTCCATGTCCCGGCCAGATGCTTGGGGCGTGA
CTAAGGGAGCAGCCGAGCTGATGCAGCAGAAGGAGGCCACTACTGAGCAGCAGCTGAGAGAGCTCTTTGA
AAAACAAAAATTCAAGTCTGCACAGTAGCCACAGGCAACAGTTTGGGGTAAATCCCTCCATGCCTGGGCC
TCAATGTGTGCCCGGAAGGAAAAGATGGCTGAAAGTCAGTGTTTGTGTTAATACCAGCCTACACCTATGA
TTGGTGCTAGTTAGACAAAATGGAATCACAAATAATAGCATTTTACAGTTACAAAACTATGTTTTAGAAA
TTTACTTAGAAGCAAAGAAAGGAAAAGTCCACGGTAAATCCTGAATAAATTTTCTAAGTACCTGCCGTGT
AACAAACAGTCATACAGAAGTCACTGCGGCAAAGAGAAAGTCACCTAGGTGTGAGTCCCATTTCAGGCAG
GAGTGTAGGACACAAACCACCATAAATGTCATAGTAACTTCAGGGACCATATCATAAAGGACATAGCAAC
CAACTTTATCTAAGGTGCTGCTGGCAGTGGATCCTAAGATCTTTTGAGCATGACCCTTAGAGAGAAACAT
CGAATGCACCAGCCCTGTAAAACTTAACATATAACCACAAAGAAGAAACAATGGACTGCTATTCTGTCTG
CTTCTATTCCATTTCATTAATAAAAATGTGCTGGTTGAAAACCTTCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5100429 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100429 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11233 same embryo
 EMAGE:11230 same embryo
 EMAGE:11229 same embryo
 EMAGE:11231 same embryo
 EMAGE:11228 same embryo
 EurExpress:euxassay_001266 same experiment
 MGI:4823470 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS