EMAGE mouse gene expression spatial database

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EMAGE:11292

Nt5c3 5'-nucleotidase, cytosolic III ( MGI:1927186)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006605. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006605_01	euxassay_006605_02	euxassay_006605_03	euxassay_006605_04

euxassay_006605_05	euxassay_006605_06	euxassay_006605_07	euxassay_006605_08	euxassay_006605_09

euxassay_006605_10	euxassay_006605_11	euxassay_006605_12	euxassay_006605_13	euxassay_006605_14

euxassay_006605_15	euxassay_006605_16	euxassay_006605_17	euxassay_006605_18	euxassay_006605_19

euxassay_006605_20	euxassay_006605_21	euxassay_006605_22	euxassay_006605_23	euxassay_006605_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11292_wholemount_strong.wlz

11292_wholemount_moderate.wlz

11292_wholemount_weak.wlz

11292_wholemount_possible.wlz

11292_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11292_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 10 18

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 09 15

cervical ganglion

weak

regional

weak expression: see section 08 09 16

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7128
Entity Detected:	Nt5c3, 5'-nucleotidase, cytosolic III ( MGI:1927186)
Sequence:	sense strand is shown >T7128 CCACGCGTCCGGCCGTACGTAGCTTGTCCGGTTACCTTCAGAAAGATTGGATAACCACGAAATGACTAAT CAGGAGTCTGCTGTCCATTTGAAAATGATGCCAGAATTTCAGAAAAGCTCAGTTCGTATCAAGAACCCTA CAAGAGTAGAAGAAATTATCTGTGGTCTCATTAAAGGAGGCGCTGCCAAACTTCAGATAATAACAGACTT TGACATGACTCTAAGTAGGTTTTCCTACAATGGGAAAAGATGCCCAACGTGTCATAATATCATTGACAAC TGTAAGCTTGTTACAGATGAATGTCGAAGAAAGTTACTGCAGTTAAAGGAGCAGTATTATGCTATTGAAG TTGATCCTGTTCTCACCGTGGAAGAGAAGTTCCCTTACATGGTAGAGTGGTATACCAAATCTCACGGTTT GCTTATTGAACAAGGCATACCAAAAGCCAAACTTAAAGAGATTGTGGCTGACTCTGACGTTATGCTCAAG GAAGGATATGAGAATTTCTTCGGTAAACTTCAACAGCACGGTATCCCTGTGTTCATATTTTCTGCTGGTA TTGGTGATGTACTTGAAGAAGTTATCCGGCAGGCTGGAGTTTATCACTCAAACGTCAAAGTAGTGTCAAA CTTCATGGATTTTGATGAAAATGGAGTGCTGAAAGGATTCAAAGGAGAGTTAATCCACGTGTTCAACAAA CACGATGGTGCCCTGAAGAATACAGACTACTTCAGCCAGCTGAAAGACAACAGCAACATCATTCTGCTCG GGGACTCCCAAGGAGACCTAAGAATGGCCGATGGCGTAGCCAATGTTGAACACATTCTGAAAATTGGTTA TCTGAATGACAGAGTAGACGAGCTTTTAGAAAAATACATGGACTCTTATGATATTGTTTTAGTAAAAGAA GAATCATTGGAGGTTGTCAACTCAATTTTACAGAAGACATTATAAACAAGCCATCGTGAGAAGACTTCCC CCAGGGGTACACCTGGATCAGGCACTGTCCATTTTGAGGAAAGACTCTTTCTTATTTATAAAACAGTCTT GCTCCTGGAACTTTTCCCTTTCCCCAACAGACGATCTTCAGACGTGTGGAACCTGTGAACCGCGGCCCCT CTTCCCACCTCCATCACTATACTCAGCAGAATTTTTAAGTCATTAAAAATTATCTTAAAGCCAAAATTAG AAAGATGGCTCCCTTTTCCAAAGTGAGTTTTGTAGTTTGGTGTTCTGCAGTTTCTAGGGCATTCTCCCAG GTGGGTGTATAAGGGCGCAGAAGACGCTTTCTGGATGTACTATGTTCCTGATTTTAACCACTGCTAGCTT TGAAATTGGGGTGGAGTATGGAGACATCATCTTCTGAAATTGTTTTATTTGTTTATTTTGTCTATAAAAT ACATTTGCCAGGAAGTTGTGTCTTAAAAACTGTTGGTGTCCGCATCGCTAACATTGCAAATACTCTGTGT GAAACACTGAGAATAAACCAGAAAAAGAAAGCTTTTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4018956 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4018956 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11293 same embryo
	EMAGE:11291 same embryo
	EMAGE:11290 same embryo
	EMAGE:11289 same embryo
	EMAGE:11294 same embryo
	EurExpress:euxassay_006605 same experiment
	MGI:4826809 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source