EMAGE mouse gene expression spatial database

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EMAGE:11311

Txnip thioredoxin interacting protein ( MGI:1889549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006657. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006657_01	euxassay_006657_02	euxassay_006657_03	euxassay_006657_04

euxassay_006657_05	euxassay_006657_06	euxassay_006657_07	euxassay_006657_08	euxassay_006657_09

euxassay_006657_10	euxassay_006657_11	euxassay_006657_12	euxassay_006657_13	euxassay_006657_14

euxassay_006657_15	euxassay_006657_16	euxassay_006657_17	euxassay_006657_18	euxassay_006657_19

euxassay_006657_20	euxassay_006657_21	euxassay_006657_22	euxassay_006657_23	euxassay_006657_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11311_wholemount_strong.wlz

11311_wholemount_moderate.wlz

11311_wholemount_weak.wlz

11311_wholemount_possible.wlz

11311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7378
Entity Detected:	Txnip, thioredoxin interacting protein ( MGI:1889549)
Sequence:	sense strand is shown >T7378 GATTTCCAGAGTTTCTCCAGTTGCGGAAGACAGCTGTTATTTTTCTCCTGAAAGCTCTTGGCACAGCCGG CAGGTTGAAACTTTCAGGCACCTTTTGGAAAAGTTGTTAGGGTTTGTTTGAAGCTTTCTTTACATTTTCG TTTGGGTTTTCAAGCCCTGACTTTACGGAGGTGAGCTCTTCGTTTGCTTTGAAGGGTTCTTAAAGATTTT TTTCCTCTCCGGCTTTCGTTTTTCTTGAACCCACTCGGCTCAATCATGGTGATGTTCAAGAAGATCAAGT CTTTTGAGGTGGTCTTCAACGACCCCGAGAAAGTGTACGGCAGCGGGGAGAAGGTGGCCGGACGGGTAAT TGTGGAAGTGTGTGAAGTTACCCGAGTCAAAGCTGTCAGGATCCTGGCTTGCGGCGTGGCCAAGGTCCTG TGGATGCAAGGGTCTCAGCAGTGCAAACAGACTTTGGACTACTTGCGCTATGAAGACACACTTCTCCTAG AAGAGCAGCCTACAGCAGGTGAGAACGAGATGGTGATCATGAGGCCTGGAAACAAATATGAGTACAAGTT CGGCTTCGAGCTTCCTAGAGGGCCCCTGGGAACATCCTTTAAAGGAAAATATGGTTGCGTAGACTACTGG GTGAAGGCTTTTCTCGATCGCCCCAGCCAGCCAACTCAAGAGGCAAAGAAAAACTTCGAAGTGATGGATC TAGTGGATGTCAATACCCCTGACTTAATGGCACCAGTGTCTGCCAAAAAGGAGAAGAAAGTTTCCTGCAT GTTCATTCCTGATGGACGTGTGTCAGTCTCTGCTCGAATTGACCGAAAAGGATTCTGTGAAGGTGATGAC ATCTCCATCCATGCTGACTTTGAGAACACGTGTTCCCGAATCGTGGTCCCCAAAGCGGCTATTGTGGCCC GACACACTTACCTTGCCAATGGCCAGACCAAAGTGTTCACTCAGAAGCTGTCCTCGGTCAGAGGCAATCA CATTATCTCAGGGACTTGCGCATCGTGGCGTGGCAAGAGCCTCAGAGTGCAGAAGATCAGACCATCCATC CTGGGCTGCAACATCCTCAAAGTCGAATACTCCTTGCTGATCTACGTCAGTGTCCCTGGCTCCAAGAAAG TCATCCTTGATCTGCCCCTAGTGATTGGCAGCAGGTCTGGTCTGAGCAGCCGGACATCCAGCATGGCCAG CCGGACGAGCTCTGAGATGAGCTGGATAGACCTAAACATCCCAGATACCCCAGAAGCTCCTCCTTGCTAT ATGGACATCATTCCTGAAGATCACAGACTAGAGAGCCCCACCACCCCTCTGCTGGACGATGTGGACGACT CTCAAGACAGCCCTATCTTTATGTACGCCCCTGAGTTCCAGTTCATGCCCCCACCCACTTACACTGAGGT GGATCCGTGCGTCCTTAACAACAACAACAACAACAACAACAACAGCAACGTGCAGTGAGCCTGCAGGAAA TGAAGCATCTGTATTAGCGCATTTCTTTCTGCCTCTCTGCTTGAACTCCAGTGTTTCAGAGACTCAGTCT CTACAGCGGGGAACGGGTACACCCCAGCCGCTGACTCCTCAAGATGGGTGGCAATCAGTAGGCAGGTCTC CGGCTTCAAGTGGTGCAGACCAGTGCCCGCACTGTGGCAAAGGAGTGTTTGCTGGGTGGATGTCAGAACA GTCTAGAAAAATTGAGACCTGACCACTTTCTCGGATGTTGGAAATGAAGAACTTGTTTGTGTTGACTGAG TCAGGGCACTGCTGACCTTCTGGCGTTGTCTTTCCAAGGTTTTTGTTTTAAAGGGAATTTTAAATTGTCT AAAATACCAGTAGACCATCATCTGTGCCATGGGGGACAGAGCCAATTTCAAGTCATGGCCAAAATTTTGT AAGAGGAGTGTTTTTGTGTGTTTTTTAAAGTCAGTGTTCCTTTTTTATATCTTTACAAAGAAAAGACCTT CCACGGCTGGTGAGCACGCAGCCTGTGAAATTCGGGGCAGCTGCTCCAAGTTGACTTCACCCTGGGAGCA GTAGTAGCTGTGCCCACTGACGGCCATAAAAGCCATTTTACAGCCAGTTGCACTGTGTTCTCTTGTAAGC ATAATCAGATGGGAGAATCTGTTATTTCCCTGTAACCCCTTGGAATTGATTCTAAGGTGATGTTCTTAGC ACTTTAGCTTGTCAGTTTTGTTTTAGTCTCCGTTATAGATGTAAGCTCCACCAGTCTCTTAAGGGTTAAG CCCAGTGACTTGGAGGGTGGGGGTTAGGGTCTCTATCCCTGAACATTGTAGACCCAGGCTGGCCTGAGAG AGAGATCCACCTGCCTCTGCCTCCTGAGTGCTGCGATCAAAGGCCCAGCTTGGTTATTGCTTTTGAGGCT TTCTCCCAACGCACAGACTTGTATAATTCTAACACTAATCCTGTGAAGGGTTGTGGTTGACAGCTGGAGC CTGGGTGACATTCTACATTGAGATGCCCCAGCACTGATCGGGGCACAGAAGCCCCCAGACCCCATTTCCT GTCCAGTGTTGGGAGAAAGTGCTGCTTTCACTGTGGCCTCAGCCCTGGCTCGGAAGCTCACTAAGCCTTA GCACTTTGTCCTGTGTCAGCTCCACCTGAGAACTGTGCAGCCAGAATGTCTGCGAGCTGATGGAGGTTTC GGTTTTATTGTTTTTGTATTTTGTGTATCTTTTTGTATGATTAAAAACTATATTTTCTACTTATCCAAAT ATATTTTCACCCCAAAGTGGGGTTATCCTTTGTAAAAAAAATAAAGTTTTTTAATGACAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193517 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193517 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11309 same embryo
	EMAGE:11307 same embryo
	EMAGE:11310 same embryo
	EMAGE:11308 same embryo
	EMAGE:11312 same embryo
	EurExpress:euxassay_006657 same experiment
	MGI:4829033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source